Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   F1614_RS06300 Genome accession   NZ_CP043847
Coordinates   1302254..1303627 (-) Length   457 a.a.
NCBI ID   WP_002438664.1    Uniprot ID   -
Organism   Staphylococcus epidermidis strain NCCP 16828     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1297254..1308627
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  F1614_RS06285 (F1614_06340) cysE 1298264..1298893 (-) 630 WP_002438669.1 serine O-acetyltransferase -
  F1614_RS06290 (F1614_06345) gltX 1299278..1300732 (-) 1455 WP_017465101.1 glutamate--tRNA ligase -
  F1614_RS06295 (F1614_06350) - 1301142..1302200 (-) 1059 WP_158171810.1 PIN/TRAM domain-containing protein -
  F1614_RS06300 (F1614_06355) radA 1302254..1303627 (-) 1374 WP_002438664.1 DNA repair protein RadA Machinery gene
  F1614_RS06305 (F1614_06360) - 1304118..1306571 (-) 2454 WP_017465099.1 ATP-dependent Clp protease ATP-binding subunit -
  F1614_RS06310 (F1614_06365) - 1306585..1307592 (-) 1008 WP_080395241.1 protein arginine kinase -
  F1614_RS06315 (F1614_06370) - 1307582..1308145 (-) 564 WP_017465097.1 UvrB/UvrC motif-containing protein -
  F1614_RS06320 (F1614_06375) - 1308166..1308627 (-) 462 WP_001832288.1 CtsR family transcriptional regulator -

Sequence

Protein


Download         Length: 457 a.a.        Molecular weight: 50273.86 Da        Isoelectric Point: 7.5278

>NTDB_id=386245 F1614_RS06300 WP_002438664.1 1302254..1303627(-) (radA) [Staphylococcus epidermidis strain NCCP 16828]
MAKKKVIFECMACGYQSPKWMGKCPNCGAWNQMEEIVEKVAHPKHGVKTKETAGKVQKLNSVKHEATPRLLTESKEFNRV
LGGGIVSGSLVLIGGDPGIGKSTLLLQICAALSQNKNVLYITGEESINQTKLRADRLEEDSSHLNVLAETDLEVIHQTVK
EERPDLLVVDSIQTIYHPEISSAPGSVSQVRESTQSLMNIAKQMNIATFIVGHVTKEGQIAGPRLLEHMVDTVLYFEGDE
HHAYRILRAVKNRFGSTNEMGIFEMKQSGLKGVLNPSEMFLEERSTNVPGSTIVPTMEGTRPLLIEVQALVTPTTFNNPR
RMATGIDHNRLSLLMAVLEKKENYLLQQQDAYIKVAGGVKLTEPAVDLSIIVATASSFKDQAVDGLDCFVGEVGLTGEVR
RVSRIEQRVQEAAKLGFKRAIIPQTNIGGWTFPEGIQVVGVSSVHEALKYALHSKQR

Nucleotide


Download         Length: 1374 bp        

>NTDB_id=386245 F1614_RS06300 WP_002438664.1 1302254..1303627(-) (radA) [Staphylococcus epidermidis strain NCCP 16828]
ATGGCCAAGAAAAAAGTAATATTCGAATGTATGGCATGTGGCTATCAATCTCCTAAGTGGATGGGCAAGTGCCCTAACTG
TGGTGCGTGGAATCAAATGGAAGAAATAGTAGAAAAGGTTGCTCATCCTAAACATGGTGTAAAAACAAAAGAAACAGCAG
GTAAGGTACAAAAGTTAAATAGTGTTAAGCATGAAGCTACCCCTAGATTACTTACAGAATCAAAGGAATTTAACCGTGTT
TTAGGTGGAGGTATTGTAAGTGGTTCGTTAGTACTAATAGGTGGAGACCCAGGTATTGGTAAGTCAACTTTATTGCTACA
AATTTGTGCTGCGCTGTCACAAAATAAGAATGTTTTATATATTACTGGTGAGGAATCTATTAATCAAACTAAGTTGCGCG
CTGATCGTTTGGAAGAAGATTCTAGTCATCTTAATGTTTTAGCCGAAACTGATTTAGAAGTGATACATCAAACTGTAAAA
GAAGAGAGACCTGACTTACTTGTTGTTGATTCGATTCAAACAATCTATCATCCGGAAATTAGTTCCGCACCTGGATCGGT
ATCACAAGTAAGAGAGAGTACGCAAAGTTTAATGAACATTGCTAAACAAATGAATATTGCCACATTTATTGTGGGACACG
TAACAAAAGAAGGTCAAATCGCTGGACCAAGATTATTGGAACATATGGTTGATACAGTTCTTTATTTTGAAGGAGATGAG
CATCACGCATATCGTATCCTTAGAGCAGTAAAAAATAGATTTGGTTCTACAAATGAGATGGGGATTTTCGAAATGAAGCA
AAGTGGATTAAAAGGTGTACTTAATCCTTCTGAAATGTTTTTAGAAGAACGTTCTACAAATGTTCCGGGCTCTACAATCG
TCCCCACTATGGAAGGAACAAGACCACTACTCATTGAAGTCCAAGCGCTTGTTACACCAACAACATTTAATAATCCTAGA
CGAATGGCTACAGGTATAGATCATAATCGATTAAGTTTACTTATGGCGGTTCTAGAAAAAAAGGAAAACTATTTACTCCA
ACAACAAGATGCCTATATTAAAGTAGCAGGTGGCGTCAAATTAACAGAACCTGCTGTTGATTTAAGCATTATTGTTGCGA
CAGCTTCAAGTTTTAAAGATCAAGCTGTTGATGGATTAGATTGTTTTGTGGGTGAAGTTGGATTAACAGGTGAAGTACGC
AGAGTATCACGCATAGAGCAACGTGTTCAAGAAGCGGCCAAACTAGGGTTTAAAAGAGCTATTATTCCACAGACAAATAT
TGGAGGTTGGACATTCCCAGAAGGCATCCAAGTCGTTGGTGTTTCATCAGTACATGAAGCTTTGAAATATGCATTACATT
CAAAACAGCGATAA

Domains

Predicted by InterproScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

Subunit ChlI of Mg-chelatase (ChlI)

(353-426)

AAA domain (AAA_25)

(71-218)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

66.009

99.781

0.659
  radA Streptococcus pneumoniae Rx1

60.802

98.249

0.597
  radA Streptococcus pneumoniae D39

60.802

98.249

0.597
  radA Streptococcus pneumoniae R6

60.802

98.249

0.597
  radA Streptococcus pneumoniae TIGR4

60.802

98.249

0.597
  radA Streptococcus mitis NCTC 12261

60.802

98.249

0.597
  radA Streptococcus mitis SK321

60.802

98.249

0.597

Multiple sequence alignment