Detailed information    

insolico Bioinformatically predicted

Overview

Name   sbcB   Type   Machinery gene
Locus tag   HHM36_RS03955 Genome accession   NZ_CP051615
Coordinates   784639..786066 (+) Length   475 a.a.
NCBI ID   WP_000980556.1    Uniprot ID   -
Organism   Escherichia coli O25b:H4-ST131 strain 2017_APHA     
Function   promote homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 779639..791066
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HHM36_RS03935 (HHM36_03930) sbmC 780779..781252 (-) 474 WP_001105368.1 DNA gyrase inhibitor SbmC -
  HHM36_RS03940 (HHM36_03935) - 781309..782529 (-) 1221 WP_000343765.1 ISL3-like element ISEc53 family transposase -
  HHM36_RS03945 (HHM36_03940) - 782548..783066 (-) 519 WP_000115885.1 ClbS/DfsB family four-helix bundle protein -
  HHM36_RS03950 (HHM36_03945) dacD 783264..784430 (-) 1167 WP_001296209.1 serine-type D-Ala-D-Ala carboxypeptidase DacD -
  HHM36_RS03955 (HHM36_03950) sbcB 784639..786066 (+) 1428 WP_000980556.1 exodeoxyribonuclease I Machinery gene
  HHM36_RS03960 (HHM36_03955) tnpA 786177..786635 (-) 459 WP_001531805.1 IS200/IS605-like element IS200C family transposase -
  HHM36_RS03965 (HHM36_03960) plaP 786852..788210 (-) 1359 WP_000019194.1 putrescine/proton symporter PlaP -
  HHM36_RS03970 (HHM36_03965) yoeI 788200..788262 (-) 63 WP_010723108.1 membrane protein YoeI -
  HHM36_RS03975 (HHM36_03970) yeeY 788477..789406 (-) 930 WP_000803366.1 LysR substrate-binding domain-containing protein -
  HHM36_RS03980 (HHM36_03975) yeeZ 789452..790276 (-) 825 WP_000754737.1 SDR family oxidoreductase -
  HHM36_RS03985 (HHM36_03980) yoeB 790359..790613 (-) 255 WP_000767819.1 type II toxin-antitoxin system mRNA interferase toxin YoeB -
  HHM36_RS03990 (HHM36_03985) yefM 790610..790861 (-) 252 WP_001259253.1 YoeB-YefM toxin-antitoxin system antitoxin YefM -

Sequence

Protein


Download         Length: 475 a.a.        Molecular weight: 54437.70 Da        Isoelectric Point: 5.4700

>NTDB_id=385688 HHM36_RS03955 WP_000980556.1 784639..786066(+) (sbcB) [Escherichia coli O25b:H4-ST131 strain 2017_APHA]
MMNDGKQQSTFLFHDYETFGTHPALDRPAQFAAIRTDNEFNVIGEPEVFYCKPADDYLPQPGAVLITGITPQEARAKGEN
EAAFAARIHSLFTVPKTCILGYNNVRFDDEVTRNVFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDG
LPSFRLEHLTKANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFDYLFTHRNKHKLMALIDVPQMKPLVHVSGMFGAWR
GNTSWVAPLAWHPENRNAVILVDLAGDISPLLELDSDTLRERLYTAKADLGDNAAVPVKLVHINKCPVLAQANTLRPEDA
DRLGINRQHCLDNLKILRENPQVREKVVAIFAEAEPFTPSDNVDAQLYNGFFSDADRAAMKIVLETEPRNLPALDITFVD
KRIEKLLFNYRARNFPGTLDYAEQQRWLEHRRQVFTPEFLQGYADELQMLAQQYADDKEKVALLKALWQYAEEIV

Nucleotide


Download         Length: 1428 bp        

>NTDB_id=385688 HHM36_RS03955 WP_000980556.1 784639..786066(+) (sbcB) [Escherichia coli O25b:H4-ST131 strain 2017_APHA]
ATGATGAATGACGGTAAGCAACAATCTACCTTTTTGTTTCACGATTACGAAACCTTTGGAACGCACCCCGCGTTAGATCG
CCCTGCACAGTTCGCAGCCATTCGCACTGATAACGAATTCAATGTCATCGGCGAACCCGAAGTCTTTTACTGCAAGCCCG
CGGATGACTATTTACCCCAGCCTGGAGCAGTATTAATTACCGGTATTACCCCGCAGGAGGCACGGGCGAAAGGAGAAAAC
GAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTACCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTT
CGACGACGAAGTCACACGCAACGTTTTTTATCGTAATTTCTACGATCCTTACGCCTGGAGCTGGCAGCATGATAACTCGC
GCTGGGATTTACTGGATGTTATGCGTGCCTGTTATGCCCTGCGCCCGGAAGGAATAAACTGGCCTGAAAATGATGACGGT
CTACCGAGCTTTCGCCTTGAGCATTTAACCAAAGCGAATGGTATTGAACATAGCAACGCCCACGATGCGATGGCTGATGT
GTACGCCACTATTGCGATGGCGAAACTGGTAAAAACGCGTCAGCCACGCCTGTTTGATTATCTCTTTACCCATCGTAATA
AACACAAACTGATGGCGTTGATTGATGTTCCGCAGATGAAACCCCTAGTTCACGTTTCCGGAATGTTTGGGGCATGGCGT
GGCAATACCAGCTGGGTGGCACCGCTGGCGTGGCATCCTGAAAATCGCAATGCCGTAATTTTGGTGGATTTGGCAGGAGA
CATTTCGCCATTACTGGAGCTGGATAGCGACACATTGCGCGAGCGTTTATATACCGCAAAAGCCGATCTTGGCGATAACG
CCGCCGTTCCGGTTAAGCTGGTGCATATCAATAAATGTCCGGTACTGGCCCAGGCGAATACGCTACGCCCGGAAGATGCC
GACCGACTGGGAATTAATCGTCAGCATTGCCTCGATAACCTAAAAATTCTGCGTGAAAATCCGCAAGTGCGTGAAAAAGT
GGTGGCGATATTCGCGGAAGCCGAACCGTTTACACCTTCAGATAACGTGGATGCACAGCTTTATAATGGCTTTTTCAGTG
ACGCCGATCGTGCAGCAATGAAAATTGTGCTGGAAACCGAGCCGCGTAATTTACCGGCACTGGATATCACTTTTGTTGAT
AAACGAATTGAAAAACTGTTGTTCAATTATCGAGCGCGCAACTTCCCGGGGACGCTGGATTATGCCGAGCAGCAACGCTG
GCTGGAGCACCGCCGCCAGGTCTTCACGCCAGAGTTTTTGCAGGGCTATGCTGATGAATTGCAGATGCTGGCACAACAGT
ATGCCGATGACAAAGAGAAAGTGGCGCTGTTAAAAGCGCTTTGGCAGTACGCGGAAGAGATCGTCTGA

Domains

Predicted by InterProScan.

Exonuclease C-terminal (ExoI_SH3)

(212-472)

Exonuclease (RNase_T)

(13-188)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  sbcB Vibrio cholerae O1 biovar El Tor strain E7946

58.936

98.947

0.583

Multiple sequence alignment