Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   F0Q02_RS00535 Genome accession   NZ_CP043573
Coordinates   117191..118576 (-) Length   461 a.a.
NCBI ID   WP_003059793.1    Uniprot ID   -
Organism   Comamonas testosteroni strain R5-28     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 112191..123576
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  F0Q02_RS00510 - 113266..114030 (+) 765 WP_003059805.1 glycosyltransferase family 25 protein -
  F0Q02_RS00515 - 114020..115105 (-) 1086 WP_003059803.1 glycosyltransferase -
  F0Q02_RS00520 - 115186..115395 (-) 210 WP_003059801.1 zinc-finger domain-containing protein -
  F0Q02_RS00525 - 115535..116473 (-) 939 WP_003059799.1 branched-chain amino acid transaminase -
  F0Q02_RS00530 - 116555..117010 (-) 456 WP_003059795.1 hypothetical protein -
  F0Q02_RS00535 radA 117191..118576 (-) 1386 WP_003059793.1 DNA repair protein RadA Machinery gene
  F0Q02_RS00545 - 119048..120355 (-) 1308 WP_003059791.1 Glu/Leu/Phe/Val family dehydrogenase -
  F0Q02_RS00550 - 120565..121488 (+) 924 WP_029158564.1 fumarylacetoacetate hydrolase family protein -
  F0Q02_RS00555 - 121654..122517 (-) 864 WP_003059787.1 PPK2 family polyphosphate kinase -

Sequence

Protein


Download         Length: 461 a.a.        Molecular weight: 48059.37 Da        Isoelectric Point: 7.6670

>NTDB_id=385224 F0Q02_RS00535 WP_003059793.1 117191..118576(-) (radA) [Comamonas testosteroni strain R5-28]
MAKEKTTFTCNACGGTSPRWLGKCPGCGAWNSLVETVADSASGGKNRLSQPQGYAGLANAQPVTPLSAIEAQDVARTPSG
IEELDRVLGGGVVEGGVVLIGGDPGIGKSTLLLQAMDALHRIGLPTLYVTGEESGAQVALRSRRLGLDNSQVNVLAEIQL
EKILATVEATQPAVVVIDSIQTVYSDQLSSAPGSVAQVRECAAHLTRAAKTTGITIILVGHVTKDGALAGPRVLEHMVDT
VLYFEGDTHSNFRLVRAIKNRFGAVNEIGVFAMTEKGLKGVTNPSAIFLSQHSEPVPGSCVLVTLEGTRPMLVEIQALVD
QGGPAARRLSVGLDRDRLAMLLAVLNRHAGVACADQDVFVNAVGGVRINEPAADLAVMLAITSSLRGKSLPKGFIAFGEV
GLAGEVRPAPRGQERLKEAAKLGFTIAVVPKANAPKKPIAGLTIHAVERVDEAINIVRGIG

Nucleotide


Download         Length: 1386 bp        

>NTDB_id=385224 F0Q02_RS00535 WP_003059793.1 117191..118576(-) (radA) [Comamonas testosteroni strain R5-28]
ATGGCCAAAGAGAAAACCACTTTCACCTGCAACGCCTGTGGCGGCACCAGCCCGCGCTGGCTGGGCAAATGCCCGGGCTG
CGGCGCCTGGAACAGCCTGGTCGAGACCGTGGCCGACTCCGCCTCGGGCGGCAAGAACCGCCTGAGCCAGCCCCAGGGCT
ATGCAGGTCTGGCCAATGCCCAGCCCGTCACCCCGCTGTCGGCCATCGAGGCCCAGGATGTGGCACGCACGCCCAGCGGC
ATCGAGGAACTGGACCGGGTGCTGGGCGGCGGCGTGGTCGAAGGCGGCGTGGTACTGATCGGCGGCGACCCTGGCATAGG
TAAGTCCACACTTCTTTTGCAGGCCATGGATGCATTGCACCGTATCGGCCTGCCCACGCTCTATGTGACGGGCGAGGAAA
GCGGTGCCCAGGTGGCGCTGCGCTCGCGCCGGCTCGGGCTGGACAACAGCCAGGTCAATGTGCTGGCCGAGATCCAGCTC
GAGAAAATCCTGGCCACCGTGGAGGCCACCCAGCCCGCCGTGGTGGTCATCGACTCGATCCAGACCGTATATTCGGACCA
GCTCTCCTCGGCTCCAGGCTCCGTGGCCCAGGTGCGCGAATGCGCGGCCCATCTCACGCGTGCGGCCAAGACCACGGGCA
TCACCATCATTCTGGTAGGCCATGTGACCAAGGACGGCGCGCTGGCAGGCCCGCGCGTGCTCGAGCACATGGTGGACACG
GTGCTCTATTTCGAAGGCGACACGCACAGCAACTTCCGCCTGGTGCGCGCCATCAAGAACCGCTTCGGTGCCGTCAACGA
GATCGGTGTCTTCGCGATGACGGAAAAAGGCCTCAAGGGCGTGACCAACCCCAGCGCCATCTTTCTGAGCCAGCACAGCG
AGCCCGTGCCCGGCAGCTGCGTGCTGGTGACGCTGGAGGGCACGCGCCCCATGCTGGTGGAGATCCAGGCCCTGGTGGAC
CAGGGCGGTCCGGCTGCGCGTCGCCTCTCGGTCGGCCTGGACCGCGACCGGCTGGCCATGCTGCTGGCCGTGCTCAACCG
CCATGCGGGCGTGGCCTGCGCCGATCAGGATGTCTTCGTCAACGCCGTGGGCGGCGTGCGCATCAACGAGCCGGCAGCCG
ACCTGGCGGTGATGTTGGCAATCACTTCCAGCCTGCGCGGCAAGTCGCTGCCCAAGGGCTTTATCGCGTTTGGCGAGGTC
GGTCTGGCCGGCGAGGTGCGCCCCGCACCGCGCGGTCAGGAGCGACTCAAGGAGGCGGCAAAGTTGGGATTCACTATCGC
CGTGGTTCCCAAGGCCAATGCTCCCAAGAAGCCCATCGCCGGCCTGACCATTCATGCCGTGGAGCGCGTGGATGAGGCCA
TCAACATCGTGCGCGGCATTGGCTGA

Domains

Predicted by InterproScan.

Lon protease (S16) C-terminal proteolytic domain (Lon_C)

(364-455)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

AAA domain (AAA_25)

(68-226)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

48.802

99.566

0.486
  radA Streptococcus mitis NCTC 12261

47.817

99.349

0.475
  radA Streptococcus pneumoniae D39

48.661

97.18

0.473
  radA Streptococcus pneumoniae TIGR4

48.661

97.18

0.473
  radA Streptococcus pneumoniae R6

48.661

97.18

0.473
  radA Streptococcus pneumoniae Rx1

48.661

97.18

0.473
  radA Streptococcus mitis SK321

49.655

94.36

0.469

Multiple sequence alignment