Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   F0P97_RS26030 Genome accession   NZ_CP043568
Coordinates   5650184..5651785 (-) Length   533 a.a.
NCBI ID   WP_182284928.1    Uniprot ID   -
Organism   Comamonas testosteroni strain T5-67     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5645184..5656785
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  F0P97_RS26005 - 5645345..5645836 (-) 492 WP_003068209.1 PIN domain-containing protein -
  F0P97_RS26010 - 5645833..5647131 (-) 1299 WP_182284924.1 THUMP domain-containing class I SAM-dependent RNA methyltransferase -
  F0P97_RS26015 - 5647311..5647649 (+) 339 WP_182284925.1 tRNA-binding protein -
  F0P97_RS26020 - 5647707..5648939 (+) 1233 WP_182284926.1 DUF711 family protein -
  F0P97_RS26025 - 5649073..5649993 (+) 921 WP_182284927.1 aspartyl/asparaginyl beta-hydroxylase domain-containing protein -
  F0P97_RS26030 rpoN/rpoN1 5650184..5651785 (-) 1602 WP_182284928.1 RNA polymerase factor sigma-54 Machinery gene
  F0P97_RS26035 lptB 5651931..5652713 (-) 783 WP_003068196.1 LPS export ABC transporter ATP-binding protein -
  F0P97_RS26040 lptA 5652710..5653366 (-) 657 WP_182284929.1 lipopolysaccharide transport periplasmic protein LptA -
  F0P97_RS26045 - 5653497..5654147 (-) 651 WP_182284930.1 thiol:disulfide interchange protein DsbA/DsbL -
  F0P97_RS26050 - 5654303..5655007 (-) 705 WP_182284931.1 SPOR domain-containing protein -

Sequence

Protein


Download         Length: 533 a.a.        Molecular weight: 59132.51 Da        Isoelectric Point: 4.6610

>NTDB_id=385201 F0P97_RS26030 WP_182284928.1 5650184..5651785(-) (rpoN/rpoN1) [Comamonas testosteroni strain T5-67]
MKPGLSLRVSQHLSLTPQLQQSIQLLQLSTLELAQEVEQMLVDNPFLERAGDEAESTGDAPVDVAASASEYNTSDSIHAE
NDAKQTQAAAETIAETPSAETPETLNWEADGMDGQDGEWGGESSSEWDTPSASSSTRNRDGDDETDAIDLAREHQSLTDF
LHRQALSLHLGAADMAALRFLIESLNDDGYLEDSLQELAQSLAGDDLEEREELEQHFVIALKLLQSLEPVGVGARDLAEC
LQLQLRDLIRTAEDEGTTEAHMNRLLLAQAICAQPMDLLARRDLRKLTSLCGAREEQLREAMALIARLEPRPGRRFVDVE
RHIVVPDVIVRPVRAGAGRMDFSVQLNPEVMPRLRVHDIYAGVLRRHKNSDGHAALQQQLQEARWFIKNIQQRFDTILRV
SEAIVERQKNFFVHGELAMRPLVLRDIADTLGLHESTISRVTTAKYMATPQGTFELKYFFGSGLGTETGGSASSTAVRAL
IKQFVDAEDAKKPLSDAKLADMLKEQGIECARRTVAKYREQLKIPTTTLRKAL

Nucleotide


Download         Length: 1602 bp        

>NTDB_id=385201 F0P97_RS26030 WP_182284928.1 5650184..5651785(-) (rpoN/rpoN1) [Comamonas testosteroni strain T5-67]
ATGAAGCCAGGTCTCTCGCTACGCGTTTCGCAGCATTTGTCGCTGACGCCGCAGCTGCAGCAATCCATTCAGCTGCTGCA
GCTCTCCACTCTGGAGCTGGCGCAGGAAGTCGAGCAGATGCTGGTCGACAACCCTTTTCTGGAACGTGCTGGCGATGAGG
CCGAATCTACGGGAGATGCTCCTGTAGATGTAGCTGCTAGCGCAAGTGAATACAACACTTCAGACAGTATTCATGCTGAA
AATGATGCGAAGCAAACGCAAGCAGCTGCTGAAACAATAGCGGAGACGCCAAGTGCCGAGACCCCGGAAACGCTGAACTG
GGAGGCCGACGGCATGGACGGCCAGGACGGGGAATGGGGCGGCGAATCCAGCAGCGAGTGGGATACGCCCTCGGCCAGCA
GCTCCACACGCAACCGCGATGGCGATGACGAAACCGATGCCATCGATCTGGCGCGCGAGCACCAGAGCCTGACCGACTTT
CTCCATCGTCAGGCGCTGAGCCTGCATCTGGGCGCGGCGGACATGGCGGCACTGCGCTTTCTCATCGAGTCGCTCAACGA
CGACGGCTATCTCGAGGATTCCCTGCAGGAGCTGGCGCAGAGCCTAGCCGGCGACGACCTGGAAGAGCGCGAGGAGCTGG
AGCAGCACTTTGTCATTGCTCTGAAGCTGCTGCAAAGCCTGGAGCCCGTGGGCGTGGGCGCGCGTGATCTGGCCGAGTGC
CTGCAGCTGCAGCTGCGCGACCTGATCCGCACCGCCGAGGACGAGGGCACGACCGAGGCGCATATGAACCGGCTGCTGCT
GGCGCAGGCCATCTGTGCGCAGCCCATGGACCTGCTGGCGCGGCGCGATCTGCGCAAGCTCACCAGCCTGTGCGGCGCGC
GCGAGGAGCAACTGCGCGAGGCCATGGCATTGATCGCCAGACTGGAGCCGCGTCCGGGGCGTCGCTTTGTCGATGTGGAA
CGCCATATCGTGGTGCCCGATGTCATCGTGCGTCCGGTGCGGGCCGGCGCCGGCCGGATGGACTTTTCCGTGCAGCTCAA
CCCCGAGGTCATGCCGCGTCTGCGCGTGCACGACATCTATGCCGGTGTGCTGCGCCGCCACAAGAACAGCGACGGCCATG
CGGCGCTGCAGCAGCAGCTGCAGGAGGCGCGCTGGTTCATCAAGAACATCCAGCAGCGCTTCGACACCATCTTGCGTGTT
TCCGAAGCCATCGTGGAGCGGCAGAAGAACTTCTTTGTGCATGGTGAGCTGGCCATGCGCCCGCTGGTGCTGCGCGATAT
CGCCGACACCCTGGGCCTGCACGAGTCCACCATCAGCCGCGTGACCACGGCCAAGTACATGGCCACGCCCCAGGGCACCT
TCGAGCTCAAGTATTTCTTCGGCTCGGGCCTGGGTACGGAAACCGGCGGCAGTGCCTCCAGCACGGCCGTGCGTGCGCTG
ATCAAGCAGTTCGTCGATGCCGAAGATGCCAAGAAGCCGCTATCGGACGCCAAGCTGGCCGATATGCTCAAAGAGCAGGG
CATCGAGTGCGCGCGCCGCACCGTCGCCAAATACCGCGAGCAGCTCAAGATACCGACGACCACGCTGCGCAAGGCGCTGT
AG

Domains

Predicted by InterproScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(375-531)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(150-360)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

50.936

100

0.51

Multiple sequence alignment