Detailed information NTG0384987

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: F0M21_RS12265
• Genome accession: NZ_CP043546
• Coordinates: 2507095..2507709 (-)
• Length: 204 a.a.
• NCBI ID: WP_012118026.1
• Uniprot ID: A7Z6X3
• Organism: Bacillus velezensis strain SYP-B637
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2502095..2512709

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
F0M21_RS12235 (F0M21_12235)	rpsT	2502398..2502664 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
F0M21_RS12240 (F0M21_12240)	holA	2502681..2503722 (-)	1042	Protein_2350	DNA polymerase III subunit delta	-
F0M21_RS12245 (F0M21_12245)	-	2503762..2503913 (-)	152	Protein_2351	hypothetical protein	-
F0M21_RS12250 (F0M21_12250)	-	2503954..2504088 (+)	135	WP_003152870.1	YqzM family protein	-
F0M21_RS12255 (F0M21_12255)	comEC	2504107..2506458 (-)	2352	WP_012118025.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
F0M21_RS12260 (F0M21_12260)	-	2506459..2507028 (-)	570	WP_003152868.1	ComE operon protein 2	-
F0M21_RS12265 (F0M21_12265)	comEA	2507095..2507709 (-)	615	WP_012118026.1	helix-hairpin-helix domain-containing protein	Machinery gene
F0M21_RS12270 (F0M21_12270)	comER	2507768..2508589 (+)	822	WP_012118027.1	late competence protein ComER	-
F0M21_RS12275 (F0M21_12275)	-	2508658..2509395 (-)	738	WP_012118028.1	class I SAM-dependent methyltransferase	-
F0M21_RS12280 (F0M21_12280)	rsfS	2509392..2509748 (-)	357	WP_007408260.1	ribosome silencing factor	-
F0M21_RS12285 (F0M21_12285)	yqeK	2509765..2510325 (-)	561	WP_012118029.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
F0M21_RS12290 (F0M21_12290)	-	2510315..2510884 (-)	570	WP_012118030.1	nicotinate-nucleotide adenylyltransferase	-
F0M21_RS12295 (F0M21_12295)	yhbY	2510895..2511185 (-)	291	WP_007408257.1	ribosome assembly RNA-binding protein YhbY	-
F0M21_RS12300 (F0M21_12300)	aroE	2511179..2512021 (-)	843	WP_012118031.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22195.40 Da
• Isoelectric Point: 6.4888

>NTDB_id=384987 F0M21_RS12265 WP_012118026.1 2507095..2507709(-) (comEA) [Bacillus velezensis strain SYP-B637]
MTKAMMTERLRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTAADKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENRPKTAAGEKENAAVNINMASLDELQAISGVG
QKKAEAIIAYREENGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=384987 F0M21_RS12265 WP_012118026.1 2507095..2507709(-) (comEA) [Bacillus velezensis strain SYP-B637]
ATGACGAAAGCGATGATGACGGAGCGGCTGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCGGCAGGGGCGGTTTT
TATCTGTATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGCCAAACAGCCTCAGCAT
CCGTCGCGGACAAAAAAGACACCGCAGCCGATAAGATTATGATTGATGTGAAAGGCGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGGGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAG
CGGCGGGTGAAAAGGAGAATGCAGCTGTCAATATCAATATGGCATCTCTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCGATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• SLBB domain (SLBB) (68-122)

• Helix-hairpin-helix motif (HHH_3) (141-202)

3D structure

• AlphaFold DB: A7Z6X3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	58.333	94.118	0.549
comEA	Latilactobacillus sakei subsp. sakei 23K	32.328	100	0.368
comEA/celA/cilE	Streptococcus mitis NCTC 12261	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae Rx1	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae D39	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae R6	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	35.545	100	0.368
comEA/celA/cilE	Streptococcus mitis SK321	35.071	100	0.363