Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   BAMF_RS21980 Genome accession   NC_014551
Coordinates   329962..330399 (-) Length   145 a.a.
NCBI ID   WP_013350968.1    Uniprot ID   -
Organism   Bacillus amyloliquefaciens DSM 7 = ATCC 23350     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 324962..335399
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BAMF_RS21955 (BAMF_0303) - 325979..327115 (+) 1137 WP_041481695.1 zinc-dependent alcohol dehydrogenase -
  BAMF_RS21960 (BAMF_0304) - 327130..327564 (+) 435 WP_013350964.1 RDD family protein -
  BAMF_RS21965 (BAMF_0305) - 327638..327961 (+) 324 WP_013350965.1 YckD family protein -
  BAMF_RS21970 (BAMF_0306) - 328066..329502 (+) 1437 WP_013350966.1 glycoside hydrolase family 1 protein -
  BAMF_RS21975 (BAMF_0307) nin/comJ 329543..329941 (-) 399 WP_013350967.1 competence protein ComJ Regulator
  BAMF_RS21980 (BAMF_0308) nucA/comI 329962..330399 (-) 438 WP_013350968.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  BAMF_RS21985 (BAMF_0309) hxlB 330751..331308 (-) 558 WP_013350969.1 6-phospho-3-hexuloisomerase -
  BAMF_RS21990 (BAMF_0310) hxlA 331305..331940 (-) 636 WP_013350970.1 3-hexulose-6-phosphate synthase -
  BAMF_RS21995 (BAMF_0311) - 332172..332534 (+) 363 WP_013350971.1 winged helix-turn-helix transcriptional regulator -

Sequence

Protein


Download         Length: 145 a.a.        Molecular weight: 16072.35 Da        Isoelectric Point: 8.4850

>NTDB_id=38455 BAMF_RS21980 WP_013350968.1 329962..330399(-) (nucA/comI) [Bacillus amyloliquefaciens DSM 7 = ATCC 23350]
MKTLKALLLVLIIIAGAVAALIKGDIFSDNEQTVQKSDYDKVILFPSDRYPETAKHIKDAIKEGHSSICTIDRDGVHKRR
EQSLKHVPVKTGYDRDEWPMAVCKQGGNNASVEYISPADNRGAGSWVGHQLTNDPDGTRVLFKIK

Nucleotide


Download         Length: 438 bp        

>NTDB_id=38455 BAMF_RS21980 WP_013350968.1 329962..330399(-) (nucA/comI) [Bacillus amyloliquefaciens DSM 7 = ATCC 23350]
TTGAAAACATTAAAAGCACTGCTGCTTGTTCTTATCATCATTGCCGGAGCAGTCGCGGCCCTGATCAAGGGTGATATTTT
CTCAGATAACGAGCAGACCGTTCAGAAGAGTGATTACGACAAGGTAATCCTATTTCCGTCCGATCGATATCCGGAAACAG
CAAAGCATATTAAGGACGCGATAAAAGAAGGACATTCCAGCATATGCACCATTGACAGAGACGGAGTACATAAGCGGCGT
GAACAATCTCTTAAACATGTGCCTGTAAAAACAGGATACGATAGGGATGAATGGCCGATGGCGGTTTGTAAACAGGGCGG
AAATAATGCCTCCGTAGAATATATTTCTCCGGCAGACAACCGCGGAGCCGGCTCTTGGGTCGGACACCAGCTTACCAATG
ATCCAGACGGAACGAGAGTATTATTTAAAATCAAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(49-131)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

73.81

86.897

0.641

Multiple sequence alignment