Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   FZP47_RS12130 Genome accession   NZ_CP043484
Coordinates   2458044..2459414 (-) Length   456 a.a.
NCBI ID   WP_149484498.1    Uniprot ID   -
Organism   Enterococcus faecium strain DMEA02     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2453044..2464414
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FZP47_RS12115 (FZP47_12115) epsC 2454364..2454900 (-) 537 WP_002293094.1 serine O-acetyltransferase EpsC -
  FZP47_RS12120 (FZP47_12120) gltX 2455223..2456683 (-) 1461 WP_002293095.1 glutamate--tRNA ligase -
  FZP47_RS12125 (FZP47_12125) - 2456853..2458010 (-) 1158 WP_002287448.1 PIN/TRAM domain-containing protein -
  FZP47_RS12130 (FZP47_12130) radA 2458044..2459414 (-) 1371 WP_149484498.1 DNA repair protein RadA Machinery gene
  FZP47_RS12135 (FZP47_12135) - 2459477..2460004 (-) 528 WP_002293097.1 dUTP diphosphatase -
  FZP47_RS12140 (FZP47_12140) proB 2460234..2461040 (+) 807 WP_002287451.1 glutamate 5-kinase -
  FZP47_RS12145 (FZP47_12145) - 2461037..2462284 (+) 1248 WP_002329828.1 glutamate-5-semialdehyde dehydrogenase -
  FZP47_RS12150 (FZP47_12150) - 2462360..2462704 (+) 345 WP_002293100.1 hypothetical protein -
  FZP47_RS12155 (FZP47_12155) - 2462959..2463279 (-) 321 WP_002293149.1 hypothetical protein -

Sequence

Protein


Download         Length: 456 a.a.        Molecular weight: 49901.59 Da        Isoelectric Point: 7.7416

>NTDB_id=384492 FZP47_RS12130 WP_149484498.1 2458044..2459414(-) (radA) [Enterococcus faecium strain DMEA02]
MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTDRRTRTSLTGEKAKPTKIADVVPKKEPRVKTKLEELN
RVLGGGVVPGSMVLIGGDPGIGKSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKIAKTNGIAIFIVGHVTKEGSIAGPRMLEHMVDTVLYFE
GEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEVANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFG
NAKRTTTGLDFNRVSLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECFIGEIGLTG
EIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAETLKRVLR

Nucleotide


Download         Length: 1371 bp        

>NTDB_id=384492 FZP47_RS12130 WP_149484498.1 2458044..2459414(-) (radA) [Enterococcus faecium strain DMEA02]
ATGGCAAAAAAATCAAAAGTACAATTTGTTTGCCAAAATTGCGGGTATGTGTCGCCTAAGTTCTTGGGACGCTGCCCAAA
TTGCGGAAAATGGAATACGATGGTCGAAGAAATCGAACAAGATACAACAGACCGCAGAACGCGAACAAGTTTGACAGGAG
AAAAAGCGAAACCAACAAAAATAGCAGACGTCGTCCCTAAAAAAGAACCTCGGGTCAAAACGAAATTAGAAGAACTGAAC
CGTGTGCTAGGTGGAGGTGTGGTACCGGGATCCATGGTCTTGATCGGTGGTGATCCAGGTATTGGTAAATCCACTTTGCT
ATTACAAGTTTCTCAGCAATTAGCAGCCATAGGCGGGAAAGTCTTGTATGTATCCGGCGAAGAAAGCGCAGAACAGATAA
AATTACGCGCGGAGCGCCTCGGAAGTATCAACACAGAATTTTATCTATACGCTGAAACAGATATGAATGAAATCAGTCGT
GCCATCGAACATATCTCCCCTGATTATGTTATTATTGATTCCATCCAAACGATGACGCAGCCAGACATCACAAGTGTGGC
AGGAAGCGTCAGCCAAGTAAGAGAAACCACTGCAGAATTACTCAAAATCGCTAAAACAAATGGGATTGCCATCTTTATCG
TCGGTCATGTTACGAAAGAAGGTTCGATCGCCGGTCCAAGAATGTTAGAGCATATGGTGGATACAGTGTTATATTTTGAA
GGTGAACAACATCATAGTTTCCGAATTTTACGAGCGGTAAAAAACCGATTTGGCTCAACGAACGAGATTGGGATTTTTGA
AATGCATGAGCATGGATTGGAAGAAGTCGCTAACCCTTCACAGATTTTCTTAGAGGAACGCCTAGACGGTGCGACTGGCT
CTGCTATCGTTGTAGCAATGGAAGGAACACGTCCAATTTTGGTAGAGATCCAAGCATTAGTCACACCTACTATGTTCGGA
AATGCTAAGCGAACAACAACAGGTCTTGATTTCAATCGTGTATCTCTGATCATGGCAGTGCTGGAAAAAAGAGCTGGACT
ACTTCTTCAGAATCAAGATGCTTACTTAAAAGCTGCAGGTGGGGTAAAATTAAATGAACCAGCGATTGATTTAGCTATCG
CTGTCAGTATTGCTTCAAGCTACAAAGAAAAAGGAACACAGCCGACGGAATGTTTCATCGGTGAAATCGGACTGACTGGA
GAAATCAGACGTGTCAGTCATATTGAACAACGGGTCAAAGAAGTCCAAAAACTCGGCTTCACAAAAGTTTACCTGCCTAA
AAACAACTTAGGCAACTGGGAAGCGCCAAAAGGAATCGAGATCGTTGGAGTAGCAACTTTGGCTGAAACGTTGAAGCGTG
TGCTCCGCTAA

Domains

Predicted by InterproScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(9-36)

Subunit ChlI of Mg-chelatase (ChlI)

(362-429)

AAA domain (AAA_25)

(70-221)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus mitis NCTC 12261

74.066

99.781

0.739
  radA Streptococcus pneumoniae Rx1

74.066

99.781

0.739
  radA Streptococcus pneumoniae D39

74.066

99.781

0.739
  radA Streptococcus pneumoniae R6

74.066

99.781

0.739
  radA Streptococcus pneumoniae TIGR4

74.066

99.781

0.739
  radA Streptococcus mitis SK321

73.846

99.781

0.737
  radA Bacillus subtilis subsp. subtilis str. 168

67.401

99.561

0.671

Multiple sequence alignment