Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   FYK34_RS11045 Genome accession   NZ_CP043473
Coordinates   2388617..2390014 (+) Length   465 a.a.
NCBI ID   WP_149296417.1    Uniprot ID   A0A5C1DH79
Organism   Chromobacterium paludis strain IIBBL 257-1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2383617..2395014
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FYK34_RS11020 (FYK34_11020) - 2385236..2386216 (+) 981 WP_149296407.1 SIS domain-containing protein -
  FYK34_RS11025 (FYK34_11025) - 2386260..2386784 (+) 525 WP_149296409.1 HAD family hydrolase -
  FYK34_RS11030 (FYK34_11030) lptC 2386781..2387350 (+) 570 WP_149296411.1 LPS export ABC transporter periplasmic protein LptC -
  FYK34_RS11035 (FYK34_11035) lptA 2387340..2387888 (+) 549 WP_149296413.1 lipopolysaccharide transport periplasmic protein LptA -
  FYK34_RS11040 (FYK34_11040) lptB 2387885..2388613 (+) 729 WP_149296415.1 LPS export ABC transporter ATP-binding protein -
  FYK34_RS11045 (FYK34_11045) rpoN/rpoN1 2388617..2390014 (+) 1398 WP_149296417.1 RNA polymerase factor sigma-54 Machinery gene
  FYK34_RS11050 (FYK34_11050) hpf 2390085..2390411 (+) 327 WP_149296419.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  FYK34_RS11055 (FYK34_11055) ptsN 2390534..2390995 (+) 462 WP_149296421.1 PTS IIA-like nitrogen regulatory protein PtsN -
  FYK34_RS11060 (FYK34_11060) hprK 2390988..2391956 (+) 969 WP_149296423.1 HPr(Ser) kinase/phosphatase -
  FYK34_RS11065 (FYK34_11065) rapZ 2391937..2392779 (+) 843 WP_149296424.1 RNase adapter RapZ -
  FYK34_RS11070 (FYK34_11070) - 2392826..2393434 (+) 609 WP_149296426.1 flavin prenyltransferase UbiX -
  FYK34_RS11075 (FYK34_11075) - 2393437..2394903 (+) 1467 WP_149296428.1 NAD(P)H-hydrate dehydratase -

Sequence

Protein


Download         Length: 465 a.a.        Molecular weight: 51369.53 Da        Isoelectric Point: 5.0872

>NTDB_id=384386 FYK34_RS11045 WP_149296417.1 2388617..2390014(+) (rpoN/rpoN1) [Chromobacterium paludis strain IIBBL 257-1]
MKQALQLKVSQQLTLTPQLQQSIKLLQLSTLDLQAEVERFLLDNPLLERGDEPRDGESASESQDSSTPDEPADSRDDSVG
ELMDWGAGSRAQGGGDDYDPMLNVPCPVSLREHLLAQLGEIALPRRDEAIVRLLIEELDDDGYLQSSLDELASHLPLELL
LDSDELAVGLALLQQFDPAGVGARTLAESLQLQLQRLPAAEPGHALALAIVRQHLALLGGRDYAKLKKLLGCTDAELRAA
QALIARLNPHPATGFHHGDVHYVIPDISVRKLKGRWVAELNRSAVPRLRVNQLYERMLADSRGQGGEMSARLQEARWLVK
NIQQRFDTILKVAEAIVERQQLFFEQGEVAMRPLILRDIAEELGLHESTVSRSTSQKYLLCSRGLFELKYFFGSSLETES
GDECSATAIKAHIRGMIEGENRAKPLSDSAIADALAKQGIQVARRTVAKYREAMQIPAVNLRKAL

Nucleotide


Download         Length: 1398 bp        

>NTDB_id=384386 FYK34_RS11045 WP_149296417.1 2388617..2390014(+) (rpoN/rpoN1) [Chromobacterium paludis strain IIBBL 257-1]
ATGAAGCAGGCACTACAGCTCAAGGTTTCGCAACAGCTGACGCTGACGCCGCAGCTGCAACAGTCCATCAAGCTGCTGCA
GCTTTCCACGCTGGACCTGCAGGCCGAGGTGGAGCGCTTCCTGCTGGACAATCCGCTATTGGAGCGCGGCGACGAGCCGC
GGGACGGCGAGTCCGCATCCGAAAGCCAGGATAGTTCGACGCCGGACGAGCCGGCCGACAGCCGCGACGACAGCGTAGGC
GAGCTGATGGACTGGGGCGCCGGCAGCCGCGCCCAGGGCGGCGGCGACGACTACGACCCCATGCTCAACGTGCCCTGCCC
GGTCAGCCTACGAGAGCACCTGCTGGCCCAGCTGGGCGAAATCGCCCTGCCCCGCCGCGACGAAGCCATCGTGCGGCTAT
TGATCGAGGAGCTGGACGACGACGGCTATCTGCAATCCAGCCTGGACGAACTGGCCAGCCATCTGCCGCTGGAACTGCTG
CTGGACAGCGACGAACTGGCGGTAGGCCTGGCGCTGCTGCAGCAGTTCGACCCGGCCGGCGTGGGCGCGCGCACGCTGGC
GGAATCGCTGCAGCTGCAATTGCAGCGCCTCCCCGCCGCGGAGCCCGGCCATGCCCTGGCGCTGGCCATCGTCCGACAAC
ACCTCGCGCTGCTGGGCGGCCGCGACTACGCCAAGCTGAAGAAGCTACTGGGCTGCACCGATGCCGAGCTGCGCGCGGCG
CAGGCCCTGATCGCGCGGCTGAACCCGCATCCGGCCACCGGCTTCCATCACGGCGATGTCCACTACGTGATCCCCGACAT
CAGCGTGCGCAAACTCAAGGGCCGCTGGGTGGCGGAGTTGAACCGCTCGGCCGTGCCGCGGCTGCGCGTCAACCAGCTGT
ACGAACGCATGCTGGCCGACAGCCGGGGCCAGGGCGGCGAAATGTCGGCCCGCCTGCAAGAGGCGCGCTGGCTGGTGAAG
AACATCCAGCAGCGGTTTGACACCATTCTGAAAGTGGCGGAAGCTATTGTTGAACGTCAACAATTGTTCTTTGAACAAGG
CGAAGTGGCGATGCGCCCGCTGATCCTGCGCGATATCGCAGAGGAACTGGGCCTGCACGAATCCACCGTATCCCGCTCCA
CCAGCCAAAAATACCTGCTTTGTTCGAGAGGTTTGTTCGAACTCAAGTATTTCTTCGGCAGTTCGTTGGAGACTGAAAGC
GGTGACGAGTGCTCCGCCACCGCCATCAAGGCTCACATCCGCGGCATGATAGAAGGCGAAAACCGGGCGAAGCCGCTGTC
CGACAGCGCCATCGCCGACGCGCTGGCCAAGCAGGGAATACAGGTTGCCAGACGCACCGTAGCCAAGTATCGTGAAGCCA
TGCAGATTCCGGCGGTGAATCTGCGCAAGGCTCTGTGA

Domains

Predicted by InterproScan.

Sigma-54 factor, core binding domain (Sigma54_CBD)

(108-294)

Sigma-54, DNA binding domain (Sigma54_DBD)

(308-463)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A5C1DH79

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

50.299

100

0.542

Multiple sequence alignment