Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   FYK34_RS09095 Genome accession   NZ_CP043473
Coordinates   1987010..1988371 (-) Length   453 a.a.
NCBI ID   WP_149296074.1    Uniprot ID   A0A5C1DGM2
Organism   Chromobacterium paludis strain IIBBL 257-1     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1982010..1993371
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FYK34_RS09065 (FYK34_09065) - 1982992..1983954 (+) 963 WP_149296068.1 hypothetical protein -
  FYK34_RS09070 (FYK34_09070) - 1983945..1984736 (-) 792 WP_149296069.1 transporter substrate-binding domain-containing protein -
  FYK34_RS09075 (FYK34_09075) - 1984890..1985240 (+) 351 WP_149296070.1 DUF2325 domain-containing protein -
  FYK34_RS09080 (FYK34_09080) - 1985285..1986190 (-) 906 WP_149296071.1 hypothetical protein -
  FYK34_RS09085 (FYK34_09085) - 1986265..1986450 (-) 186 WP_149296072.1 zf-HC2 domain-containing protein -
  FYK34_RS09090 (FYK34_09090) - 1986447..1987007 (-) 561 WP_149296073.1 sigma-70 family RNA polymerase sigma factor -
  FYK34_RS09095 (FYK34_09095) radA 1987010..1988371 (-) 1362 WP_149296074.1 DNA repair protein RadA Machinery gene
  FYK34_RS09100 (FYK34_09100) - 1988434..1988967 (-) 534 WP_149296075.1 hypothetical protein -
  FYK34_RS09105 (FYK34_09105) - 1988982..1989743 (-) 762 WP_149296076.1 SDR family oxidoreductase -
  FYK34_RS09110 (FYK34_09110) hpnE 1989740..1991011 (-) 1272 WP_149296077.1 hydroxysqualene dehydroxylase HpnE -
  FYK34_RS09115 (FYK34_09115) hpnD 1991008..1991853 (-) 846 WP_149296078.1 presqualene diphosphate synthase HpnD -
  FYK34_RS09120 (FYK34_09120) hpnC 1991919..1992731 (-) 813 WP_149296079.1 squalene synthase HpnC -

Sequence

Protein


Download         Length: 453 a.a.        Molecular weight: 48765.09 Da        Isoelectric Point: 7.0938

>NTDB_id=384384 FYK34_RS09095 WP_149296074.1 1987010..1988371(-) (radA) [Chromobacterium paludis strain IIBBL 257-1]
MAKSKTVFSCTECGGQSPKWQGQCPHCNAWNTLVEAVAAPAAASPRFQSWAANVTKVQKLSEVQTEETPRDPSGIEELDR
VLGGGIVRGAVVLIGGDPGIGKSTLLLQALSQIGQSRKVLYVSGEESAQQIALRASRLALDTSRVDLLAEICVENILSTL
KREQPEVVVIDSIQTLYTEHVTSAPGSVSQVRECAAQLTRMAKQSGITVLLVGHVTKEGSLAGPRVLEHMVDTVLYFEGD
SHSSYRMIRAIKNRFGAVNELGVFAMTDRGLKGVSNPSAIFLSSYRDDVAGSCVLVTQEGTRPLLVEIQALVDDCHGFQP
KRLTVGLEQNRLAMLLAVLHRHGGVACFDQDVFLNAVGGVKINEPAADLAIILAMVSSLRNKALPEKLVVFGEVGLAGEV
RPVTRGQERLKEAAKLGFTRAIVPSANKPRQEIEGLKVLAVDRLDQAVEFCRE

Nucleotide


Download         Length: 1362 bp        

>NTDB_id=384384 FYK34_RS09095 WP_149296074.1 1987010..1988371(-) (radA) [Chromobacterium paludis strain IIBBL 257-1]
ATGGCCAAGAGCAAAACCGTATTCAGCTGCACCGAGTGCGGCGGACAGTCCCCCAAGTGGCAGGGCCAATGCCCGCACTG
CAATGCCTGGAACACCCTGGTGGAAGCCGTGGCCGCGCCGGCCGCGGCCAGTCCGCGCTTCCAGTCCTGGGCGGCCAATG
TCACCAAGGTGCAGAAGCTGTCCGAGGTGCAAACCGAAGAAACCCCGCGCGACCCTTCCGGCATTGAGGAGCTGGACCGC
GTGCTGGGCGGCGGCATCGTGCGCGGCGCCGTGGTGCTGATAGGCGGCGATCCCGGCATCGGCAAGTCCACGCTGCTGCT
GCAGGCCCTGTCGCAGATCGGCCAGAGCCGCAAGGTGCTGTACGTGTCCGGCGAGGAGTCGGCGCAGCAGATCGCGTTGC
GCGCCTCGCGCCTGGCGCTGGATACCAGCCGGGTGGATCTGCTCGCCGAAATCTGCGTCGAGAATATCCTGTCCACGCTG
AAGCGCGAGCAGCCGGAAGTGGTGGTGATCGACTCCATCCAGACCTTGTACACCGAGCATGTGACCTCGGCGCCGGGTTC
GGTGTCGCAGGTGCGCGAATGCGCGGCGCAGCTGACGCGCATGGCCAAGCAGAGCGGCATCACCGTGCTGCTGGTGGGCC
ATGTCACCAAGGAAGGCTCGCTGGCCGGCCCGCGCGTGCTGGAGCACATGGTGGACACCGTGCTGTATTTCGAGGGCGAT
TCCCATTCCAGCTACCGCATGATACGGGCGATCAAGAACCGCTTCGGCGCGGTCAACGAGCTGGGCGTGTTCGCCATGAC
CGATCGCGGCCTGAAAGGCGTGTCCAACCCGTCCGCCATCTTCCTGTCTTCCTACCGCGACGATGTGGCCGGCTCCTGCG
TGCTGGTGACGCAGGAAGGCACGCGGCCCTTGCTGGTGGAGATCCAGGCGCTGGTGGACGACTGCCACGGCTTCCAGCCC
AAGCGTCTCACCGTCGGCCTGGAGCAGAACCGGCTGGCCATGCTGCTGGCGGTGCTGCACCGCCATGGCGGCGTGGCCTG
TTTCGACCAGGACGTATTCCTCAACGCAGTGGGCGGCGTCAAGATCAATGAGCCGGCGGCCGATCTCGCCATCATCCTGG
CCATGGTTTCCTCCTTGCGCAACAAGGCGCTGCCGGAGAAGCTGGTGGTGTTCGGCGAGGTGGGCCTGGCCGGCGAGGTG
CGCCCGGTGACGCGCGGGCAGGAGCGGCTGAAGGAAGCGGCCAAGCTGGGCTTCACCCGCGCCATCGTGCCGTCCGCCAA
CAAGCCGCGGCAGGAGATAGAGGGGCTGAAGGTGCTGGCGGTGGATAGGCTGGACCAGGCTGTGGAATTCTGCAGGGAGT
GA

Domains

Predicted by InterproScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

Subunit ChlI of Mg-chelatase (ChlI)

(360-427)

AAA domain (AAA_25)

(65-219)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A5C1DGM2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

50.439

100

0.508
  radA Streptococcus mitis NCTC 12261

48.23

99.779

0.481
  radA Streptococcus mitis SK321

48.23

99.779

0.481
  radA Streptococcus pneumoniae Rx1

50.236

93.598

0.47
  radA Streptococcus pneumoniae D39

50.236

93.598

0.47
  radA Streptococcus pneumoniae R6

50.236

93.598

0.47
  radA Streptococcus pneumoniae TIGR4

50.236

93.598

0.47

Multiple sequence alignment