Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   DEO27_RS22920 Genome accession   NZ_CP043450
Coordinates   5742441..5743853 (+) Length   470 a.a.
NCBI ID   WP_112568981.1    Uniprot ID   A0A5C1I3F6
Organism   Mucilaginibacter rubeus strain P1     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 5737441..5748853
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DEO27_RS22900 (DEO27_022900) - 5738227..5738466 (+) 240 WP_112568974.1 hypothetical protein -
  DEO27_RS22905 (DEO27_022905) - 5738450..5738821 (-) 372 WP_190295201.1 barstar family protein -
  DEO27_RS22910 (DEO27_022910) - 5739103..5740128 (+) 1026 WP_112568977.1 hypothetical protein -
  DEO27_RS22915 (DEO27_022915) - 5740058..5742343 (+) 2286 WP_146749987.1 hypothetical protein -
  DEO27_RS22920 (DEO27_022920) radA 5742441..5743853 (+) 1413 WP_112568981.1 DNA repair protein RadA Machinery gene
  DEO27_RS22925 (DEO27_022925) - 5743930..5744211 (-) 282 WP_112568983.1 hypothetical protein -
  DEO27_RS22930 (DEO27_022930) - 5744362..5746236 (+) 1875 WP_112568985.1 M61 family metallopeptidase -
  DEO27_RS22935 (DEO27_022935) - 5746239..5747546 (+) 1308 WP_112568987.1 M28 family metallopeptidase -
  DEO27_RS22940 (DEO27_022940) - 5747761..5748291 (+) 531 WP_112568989.1 AAA family ATPase -

Sequence

Protein


Download         Length: 470 a.a.        Molecular weight: 51588.35 Da        Isoelectric Point: 6.2540

>NTDB_id=384129 DEO27_RS22920 WP_112568981.1 5742441..5743853(+) (radA) [Mucilaginibacter rubeus strain P1]
MAKSKIAYFCQSCGFESPKWLGKCPSCQQWNTFVEEVIEKGNASVPAWKPASTSQQRANKPVPVTEITYSEEHRLPTPDK
EFNRVLGGGIVPGSLVLIGGEPGIGKSTLMLQLALNMPNQKVLYVSGEESERQIKMRAERLASPEGGPIGEGAYILTETS
TQNIFKQIEALEPDMVVIDSIQTLHSAHIESTPGSVSQVRECTAELLRFAKETSTPVFLIGHITKDGMIAGPKILEHMVD
TVLQFEGDRHHVYRILRTIKNRFGSASELGIYEMMGEGLREVSNPSEILLSHREAPLSGITISATLEGMRPMLIETQALV
SASAYGTPQRTATGFDTKRMSMLLAVLEKRCGFRLGAKDVFLNITGGIRVEDPAIDLGLAAAIISSHEDMPIPFKTCFAG
ELGLSGEIRAVNRVEQRIAEAQKLGFNQIFISKYNLPASGQDKKRMDLSRYDIDIKIVSSIEEVFSLLFG

Nucleotide


Download         Length: 1413 bp        

>NTDB_id=384129 DEO27_RS22920 WP_112568981.1 5742441..5743853(+) (radA) [Mucilaginibacter rubeus strain P1]
ATGGCCAAATCCAAGATCGCGTACTTTTGTCAAAGCTGTGGCTTTGAATCGCCCAAATGGCTGGGTAAATGCCCGTCATG
CCAGCAATGGAACACCTTTGTTGAAGAGGTGATTGAAAAGGGCAATGCATCAGTACCGGCCTGGAAACCTGCCTCAACCA
GTCAGCAGCGGGCCAATAAACCTGTGCCGGTTACGGAGATTACTTATTCCGAAGAGCATCGTTTACCTACTCCCGATAAA
GAGTTTAACCGTGTTTTAGGCGGCGGCATTGTTCCGGGTTCATTAGTACTTATCGGCGGCGAGCCGGGCATAGGCAAATC
AACCCTGATGTTGCAGCTTGCCCTCAACATGCCTAACCAAAAGGTGCTGTATGTGTCGGGCGAGGAAAGCGAACGACAGA
TTAAGATGAGGGCAGAAAGGTTAGCATCGCCCGAAGGGGGGCCTATCGGCGAAGGAGCCTATATCCTCACAGAAACCTCA
ACCCAAAACATTTTTAAGCAGATAGAGGCATTGGAACCAGATATGGTAGTGATAGACTCTATTCAAACGCTGCATTCGGC
ACATATCGAATCAACACCAGGCAGCGTATCCCAGGTTAGGGAATGTACTGCCGAACTGCTTCGTTTTGCCAAAGAAACCT
CAACTCCTGTATTTTTAATTGGCCACATTACCAAAGATGGTATGATAGCAGGCCCAAAAATACTGGAGCATATGGTTGAT
ACCGTGTTACAGTTTGAGGGCGACAGGCATCATGTTTATCGCATATTGCGCACTATTAAAAACCGTTTCGGCTCGGCATC
GGAGCTGGGTATTTATGAAATGATGGGCGAGGGTTTGAGGGAAGTATCCAATCCTTCGGAGATATTGCTTTCGCACCGGG
AAGCACCACTGAGCGGCATTACTATTTCTGCCACTTTAGAAGGGATGCGACCGATGCTGATTGAAACCCAGGCATTGGTT
AGTGCGTCTGCCTATGGTACCCCGCAACGTACCGCTACCGGCTTTGATACCAAACGCATGAGCATGCTGCTGGCTGTGCT
GGAAAAGCGCTGCGGTTTCAGGTTGGGCGCAAAAGATGTATTCCTGAACATTACCGGAGGCATCAGGGTTGAAGACCCCG
CTATTGATCTGGGGCTTGCAGCGGCCATTATTTCATCGCACGAGGATATGCCAATACCCTTCAAAACCTGTTTTGCCGGC
GAGCTTGGCCTTTCGGGCGAGATCAGGGCGGTAAACCGGGTAGAGCAGCGTATTGCCGAAGCACAAAAACTCGGCTTCAA
CCAAATCTTTATCTCCAAATACAACCTCCCTGCAAGCGGTCAGGATAAAAAGCGCATGGACCTCTCGCGCTACGATATCG
ACATCAAGATCGTAAGCAGTATTGAGGAAGTATTCAGTTTGTTGTTTGGATAG

Domains

Predicted by InterproScan.

Subunit ChlI of Mg-chelatase (ChlI)

(346-435)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

AAA domain (AAA_25)

(68-228)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A5C1I3F6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

54.566

93.191

0.509
  radA Streptococcus mitis NCTC 12261

49.894

100

0.5
  radA Streptococcus pneumoniae Rx1

49.894

100

0.5
  radA Streptococcus pneumoniae D39

49.894

100

0.5
  radA Streptococcus pneumoniae R6

49.894

100

0.5
  radA Streptococcus pneumoniae TIGR4

49.894

100

0.5
  radA Streptococcus mitis SK321

52.055

93.191

0.485

Multiple sequence alignment