Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   HC659_RS12715 Genome accession   NZ_CP051465
Coordinates   2492794..2493204 (-) Length   136 a.a.
NCBI ID   WP_009967785.1    Uniprot ID   P42983
Organism   Bacillus subtilis subsp. subtilis strain UCMB5121     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 2487794..2498204
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HC659_RS12685 (HC659_25100) - 2487906..2488046 (+) 141 WP_003226124.1 sporulation histidine kinase inhibitor Sda -
  HC659_RS12690 (HC659_25110) yqeF 2488352..2489083 (-) 732 WP_003229964.1 SGNH/GDSL hydrolase family protein -
  HC659_RS12695 (HC659_25120) cwlH 2489335..2490087 (-) 753 WP_032722147.1 N-acetylmuramoyl-L-alanine amidase CwlH -
  HC659_RS12700 (HC659_25130) yqeD 2490274..2490900 (+) 627 WP_015714297.1 TVP38/TMEM64 family protein -
  HC659_RS12705 (HC659_25140) gnd 2490919..2491787 (-) 869 Protein_2465 phosphogluconate dehydrogenase (NAD(+)-dependent, decarboxylating) -
  HC659_RS12710 (HC659_25150) yqeB 2492039..2492761 (+) 723 WP_010886572.1 hypothetical protein -
  HC659_RS12715 (HC659_25160) nucA/comI 2492794..2493204 (-) 411 WP_009967785.1 sporulation-specific Dnase NucB Machinery gene
  HC659_RS12720 - 2493400..2493747 (+) 348 Protein_2468 sigma-70 family RNA polymerase sigma factor -
  HC659_RS12725 (HC659_25170) spoIVCA 2493778..2495238 (-) 1461 WP_223257626.1 site-specific DNA recombinase SpoIVCA -
  HC659_RS21000 - 2495196..2495374 (-) 179 Protein_2470 hypothetical protein -
  HC659_RS12730 (HC659_25180) arsC 2495729..2496148 (-) 420 WP_004398596.1 thioredoxin-dependent arsenate reductase -
  HC659_RS12735 (HC659_25190) acr3 2496160..2497200 (-) 1041 WP_032722149.1 arsenite efflux transporter Acr3 -
  HC659_RS12740 (HC659_25200) arsK 2497223..2497663 (-) 441 WP_032722151.1 ArsI/CadI family heavy metal resistance metalloenzyme -
  HC659_RS12745 (HC659_25210) arsR 2497722..2498039 (-) 318 WP_029318103.1 arsenical resistance operon transcriptional regulator ArsR -

Sequence

Protein


Download         Length: 136 a.a.        Molecular weight: 14967.97 Da        Isoelectric Point: 5.1853

>NTDB_id=383754 HC659_RS12715 WP_009967785.1 2492794..2493204(-) (nucA/comI) [Bacillus subtilis subsp. subtilis strain UCMB5121]
MKKWMAGLFLAAAVLLCLMVPQQIQGASSYDKVLYFPLSRYPETGSHIRDAIAEGHPDICTIDRDGADKRREESLKGIPT
KPGYDRDEWPMAVCEEGGAGADVRYVTPSDNRGAGSWVGNQMSSYPDGTRVLFIVQ

Nucleotide


Download         Length: 411 bp        

>NTDB_id=383754 HC659_RS12715 WP_009967785.1 2492794..2493204(-) (nucA/comI) [Bacillus subtilis subsp. subtilis strain UCMB5121]
ATGAAAAAATGGATGGCAGGCCTGTTTCTTGCTGCAGCAGTTCTTCTTTGTTTAATGGTTCCGCAGCAGATCCAAGGCGC
ATCTTCGTATGACAAAGTGTTATATTTTCCGCTGTCTCGTTATCCGGAAACCGGCAGTCATATTAGGGATGCGATTGCAG
AGGGACATCCAGATATTTGTACCATTGATAGAGATGGAGCAGACAAAAGGCGGGAGGAATCTTTAAAGGGAATCCCGACG
AAGCCGGGCTATGACCGGGATGAGTGGCCGATGGCTGTCTGCGAGGAAGGCGGCGCAGGGGCTGATGTCCGATATGTGAC
GCCTTCTGATAATCGCGGCGCCGGCTCGTGGGTAGGGAATCAAATGAGCAGCTATCCTGACGGCACCAGAGTGCTGTTTA
TTGTGCAGTAA

Domains

Predicted by InterProScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(40-130)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P42983

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

62.609

84.559

0.529

Multiple sequence alignment