Detailed information    

insolico Bioinformatically predicted

Overview

Name   recO   Type   Machinery gene
Locus tag   FX981_RS15045 Genome accession   NZ_CP043404
Coordinates   2983261..2984031 (+) Length   256 a.a.
NCBI ID   WP_044332056.1    Uniprot ID   A0A5C0WLP6
Organism   Bacillus safensis strain PgKB20     
Function   ssDNA annealing; plasmid transformation (predicted from homology)   
DNA processing Homologous recombination

Genomic Context

Location: 2978261..2989031
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FX981_RS15015 (FX981_03016) - 2978568..2980679 (+) 2112 WP_370880885.1 HD family phosphohydrolase -
  FX981_RS15020 (FX981_03017) ybeY 2980680..2981159 (+) 480 WP_024423021.1 rRNA maturation RNase YbeY -
  FX981_RS15025 (FX981_03018) - 2981137..2981523 (+) 387 WP_024423020.1 diacylglycerol kinase family protein -
  FX981_RS15030 (FX981_03019) - 2981637..2982035 (+) 399 WP_008356263.1 cytidine deaminase -
  FX981_RS15035 (FX981_03020) era 2982028..2982933 (+) 906 WP_007501305.1 GTPase Era -
  FX981_RS15040 (FX981_03021) - 2983082..2983225 (+) 144 WP_003217288.1 YqzL family protein -
  FX981_RS15045 (FX981_03022) recO 2983261..2984031 (+) 771 WP_044332056.1 DNA repair protein RecO Machinery gene
  FX981_RS15050 (FX981_03023) glyQ 2984361..2985248 (+) 888 WP_073206339.1 glycine--tRNA ligase subunit alpha -
  FX981_RS15055 (FX981_03024) glyS 2985241..2987286 (+) 2046 WP_149126466.1 glycine--tRNA ligase subunit beta -
  FX981_RS15060 (FX981_03025) - 2987454..2988092 (+) 639 WP_024423017.1 helix-turn-helix transcriptional regulator -
  FX981_RS15065 (FX981_03026) - 2988118..2988939 (+) 822 WP_008342399.1 pyruvate, water dikinase regulatory protein -

Sequence

Protein


Download         Length: 256 a.a.        Molecular weight: 29337.98 Da        Isoelectric Point: 8.1204

>NTDB_id=383525 FX981_RS15045 WP_044332056.1 2983261..2984031(+) (recO) [Bacillus safensis strain PgKB20]
MLIKSEGIVLRTTDYGETNKIVTLLTREHGKIGVMARGAKKSNSRLSAISQPFLYGTFLIQSSTGLGTLQQGEMIESMRT
IREDLFLTAYAAYMTELMDKGTEEKKPNPYLFELLLQSLRHLNEGTDADIILFIVEVKMLSVMGMKPELDQCVHCGQKEG
QFHFSIRDNGFICQNCFSKDPYKLPLSPAAARLLRLFHYFDLSRLGQVDVKQQTKQEIRQVLDHYYDEYSGLYLKSKKFM
NQMESMKKLMGDENKS

Nucleotide


Download         Length: 771 bp        

>NTDB_id=383525 FX981_RS15045 WP_044332056.1 2983261..2984031(+) (recO) [Bacillus safensis strain PgKB20]
ATGCTCATTAAAAGTGAAGGAATCGTCCTTAGAACAACAGATTATGGCGAAACAAACAAAATTGTCACGCTGCTCACTAG
AGAACATGGAAAAATTGGCGTGATGGCCAGAGGCGCTAAAAAATCAAACAGCCGCCTGTCAGCCATAAGCCAGCCGTTTT
TATACGGCACCTTTCTCATCCAATCATCAACAGGACTTGGAACGCTCCAGCAAGGCGAAATGATTGAAAGTATGCGGACC
ATTCGGGAAGACTTATTTCTGACCGCATATGCCGCATATATGACGGAATTAATGGATAAAGGGACAGAAGAAAAAAAGCC
AAACCCTTATTTGTTTGAGCTTCTGCTTCAATCTCTGCGTCATCTCAACGAAGGCACAGATGCTGATATTATTTTATTTA
TCGTAGAGGTCAAAATGTTATCAGTGATGGGGATGAAGCCTGAACTTGATCAATGTGTCCACTGTGGTCAAAAAGAAGGG
CAGTTCCATTTCTCGATAAGAGATAACGGCTTCATTTGCCAAAATTGTTTTTCGAAAGATCCATATAAACTGCCCTTATC
CCCCGCAGCTGCAAGGCTACTCCGTTTGTTTCATTATTTTGATTTATCAAGGCTTGGTCAGGTTGATGTGAAACAGCAGA
CGAAACAAGAAATTCGTCAAGTGCTTGATCACTACTATGACGAATATTCCGGCCTCTATTTAAAATCAAAAAAATTCATG
AATCAGATGGAATCCATGAAAAAGCTAATGGGTGACGAAAACAAAAGTTGA

Domains

Predicted by InterproScan.

Recombination protein O N terminal (RecO_N)

(1-77)

Recombination protein O C terminal (RecO_C)

(82-237)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A5C0WLP6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recO Bacillus subtilis subsp. subtilis str. 168

75.391

100

0.754

Multiple sequence alignment