Detailed information    

insolico Bioinformatically predicted

Overview

Name   addA   Type   Machinery gene
Locus tag   FX981_RS02410 Genome accession   NZ_CP043404
Coordinates   453724..457428 (-) Length   1234 a.a.
NCBI ID   WP_149125791.1    Uniprot ID   -
Organism   Bacillus safensis strain PgKB20     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 448724..462428
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FX981_RS02395 (FX981_00483) - 448810..449109 (-) 300 WP_024425503.1 hypothetical protein -
  FX981_RS02400 (FX981_00484) - 449130..452525 (-) 3396 WP_149125789.1 AAA family ATPase -
  FX981_RS02405 (FX981_00485) sbcD 452522..453697 (-) 1176 WP_149125790.1 exonuclease subunit SbcD -
  FX981_RS02410 (FX981_00486) addA 453724..457428 (-) 3705 WP_149125791.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  FX981_RS02415 (FX981_00487) addB 457406..460915 (-) 3510 WP_149125792.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  FX981_RS02420 (FX981_00488) - 461068..461514 (+) 447 WP_024425498.1 ferritin-like domain-containing protein -
  FX981_RS02425 (FX981_00489) - 461713..462123 (-) 411 WP_024425497.1 DoxX family protein -

Sequence

Protein


Download         Length: 1234 a.a.        Molecular weight: 142262.34 Da        Isoelectric Point: 5.0334

>NTDB_id=383488 FX981_RS02410 WP_149125791.1 453724..457428(-) (addA) [Bacillus safensis strain PgKB20]
MQIPKPNNSTWTDDQWEAIVSEGQDILVAAAAGSGKTAVLVERLIRKMTRPEDPVDVDRLLVVTFTNASAAEMKHRITEA
LEKELAQNPGSLHMRRQLSLMNRANISTLHSFCLQVLRTFYYEIDLDPGFRLADQTEGELLGDEVLDELFEDEYKAGKPA
FFELVDRYTSDRHDLDLQWLVKRIYEFSRSHPSPEQWMRAFLSLYDVDAQTKVEQLPFYPYIKEDLSLVFRSCLELLERA
LALSKEPGGPAPRAENFIDDLEQVNELIRHQDDFEKLYELLPNINFKRLKTCKGDEYDPVLLEKATDARNQAKKQLEKLK
DEYFMRSPAQHLKSLAEMKPVVETLVELVIQFGQRFERAKQEKSIVDFSDLEHYCLRILAEQDAEGNLIETEAAKYYQQQ
FEEVLVDEYQDTNLVQETILKLVSKGEHAAEGNLFMVGDVKQSIYRFRLAEPMLFLNKYKQFTPDGKETGKRIDLNKNFR
SRSDVLDSTNFLFKQLMGETVGEIEYDEQAELKLGASYPESEDTTTEMLLVHLDQQEAETDEEREELETVQLEARVIAKK
IRQLVEQPFQVYDSKQQMTRNLQYRDIVILLRSMPWAPQMMEELKKQGIPVYANLSSGYFEATEVSVILSLLKVIDNPYQ
DIPLAAVLRSPIVHLDENEMALIRTSDKKGTYYDAVKAFMSVTHSDHPTCKKLERFFRLLRKWRDFSMNHSVAELIWEVY
RDTQYLDYVGGMPGGKQRQANLRALYDRAKQYEKAAFRGLFRFLRFIERMQERGDDLGAAKTFSETEDVVRMMTIHSSKG
LEFPVVFTAGLGRNFNMMDLNQSYLLDKELGFGSKYIHPELRISYATLPLVAMKKKMRKELLSEELRVLYVALTRAKEKL
FLVGSVKNQVKALSKWQNAATGEDWLLPDFERYQSKTYLDFIGPALIRHQAMSSILEETGEVVLSHPSAFTISFTQASDL
LKEDMSLEKKQQDEVVQALMDGLPVEGYGDADKKVAERLSWRYPYLATSQVGTKQSVSEIKRMKEIQDEYSVPSSLRRAH
ATLYDRPAFMKKKALTAAEQGTAMHTVMQHIPLSSDEPLDEIHIHQLLDSLQQRDLLTNEQVQSIDQEGISAFFSTSIGQ
KLLKADWVKREVSFSMVLPVKEVYSHIDAEGEPVLIQGMIDCLFETDGKLYLLDYKTDRVQGRYTGGLEAAVPILKKRYE
TQIALYAKAVERLTNRTLEEKILYFFDGNLEISL

Nucleotide


Download         Length: 3705 bp        

>NTDB_id=383488 FX981_RS02410 WP_149125791.1 453724..457428(-) (addA) [Bacillus safensis strain PgKB20]
ATGCAAATACCAAAACCGAATAACAGTACTTGGACGGATGACCAGTGGGAAGCCATCGTTTCAGAAGGACAAGATATCCT
TGTTGCGGCGGCGGCAGGGTCAGGTAAAACAGCTGTTTTGGTTGAACGGCTGATTCGAAAAATGACCCGTCCTGAAGATC
CAGTTGATGTGGATCGTCTGCTCGTTGTGACTTTCACAAATGCATCTGCGGCAGAGATGAAGCATCGGATCACAGAAGCA
CTTGAAAAGGAATTAGCCCAAAACCCCGGGTCCCTTCATATGAGAAGACAGCTGTCGCTCATGAATCGTGCCAATATTTC
CACCCTGCACTCCTTTTGCTTGCAGGTTTTGCGCACCTTTTACTATGAGATTGACCTAGATCCAGGCTTTCGTTTGGCTG
ATCAAACAGAAGGAGAATTATTAGGAGATGAAGTGTTAGATGAACTGTTTGAGGATGAATATAAGGCTGGAAAGCCAGCG
TTCTTTGAACTGGTTGATCGCTACACTTCTGATCGTCATGATTTAGACTTGCAATGGCTTGTAAAGAGAATTTATGAGTT
TTCCAGATCTCACCCTTCACCTGAGCAATGGATGAGGGCATTCCTTTCTCTTTATGATGTAGATGCTCAAACAAAAGTAG
AACAATTACCGTTTTACCCATATATCAAAGAGGATCTTTCTCTCGTTTTTCGAAGCTGTCTGGAACTGCTCGAACGAGCT
CTCGCCCTATCAAAAGAACCGGGCGGGCCGGCACCGAGAGCAGAAAATTTTATTGATGATTTAGAGCAAGTCAATGAATT
AATCAGGCACCAAGATGATTTTGAGAAGCTTTACGAGCTTTTGCCAAACATCAATTTTAAAAGGCTCAAAACATGTAAAG
GGGACGAATATGACCCTGTGCTGTTAGAAAAAGCCACAGATGCACGCAATCAAGCAAAAAAACAATTAGAAAAGCTTAAA
GATGAATACTTTATGCGCAGTCCGGCCCAGCATTTGAAAAGCTTAGCAGAAATGAAGCCGGTGGTCGAGACACTTGTGGA
GCTTGTCATCCAATTTGGACAGCGTTTCGAAAGAGCGAAGCAAGAAAAATCAATCGTGGATTTTTCGGATTTAGAGCATT
ATTGCTTACGCATTTTAGCAGAGCAGGACGCGGAAGGAAATTTGATCGAAACAGAAGCGGCCAAGTACTATCAACAGCAG
TTTGAAGAAGTACTCGTTGATGAATATCAAGATACAAACCTTGTGCAAGAAACGATTTTAAAGCTTGTATCTAAAGGGGA
ACATGCTGCAGAGGGCAACCTGTTTATGGTTGGTGATGTAAAACAGTCCATTTATCGTTTTAGACTGGCTGAGCCCATGC
TCTTTTTAAACAAATATAAACAATTTACACCAGACGGCAAAGAAACAGGGAAGAGAATTGATTTAAATAAAAACTTCCGC
AGCCGTTCAGATGTGTTAGACAGTACGAACTTTTTGTTCAAACAGCTGATGGGGGAAACAGTTGGAGAAATTGAATATGA
TGAACAAGCTGAATTAAAGCTTGGAGCGAGTTATCCAGAAAGCGAAGATACAACGACAGAAATGCTTCTTGTACATTTAG
ATCAACAGGAAGCAGAAACTGACGAAGAACGAGAAGAGCTAGAAACCGTACAGCTTGAAGCGAGAGTCATTGCTAAAAAA
ATAAGACAATTAGTGGAGCAGCCATTTCAAGTGTATGATTCAAAACAGCAAATGACCCGTAACTTGCAGTATCGGGACAT
TGTGATTTTGCTTCGCTCCATGCCGTGGGCACCGCAAATGATGGAGGAATTGAAGAAGCAGGGAATCCCTGTGTATGCGA
ATCTTTCCTCTGGTTATTTTGAAGCAACAGAGGTGTCTGTGATTCTTTCTTTGTTAAAAGTCATTGATAATCCATATCAA
GATATCCCGCTTGCAGCTGTATTAAGATCACCAATTGTTCATTTAGACGAAAATGAAATGGCACTGATCCGCACGAGTGA
TAAAAAAGGCACCTATTATGATGCTGTGAAAGCATTTATGAGTGTGACTCATTCAGATCACCCTACATGCAAAAAACTAG
AACGATTTTTCAGGTTGTTGCGTAAGTGGCGTGATTTCTCTATGAATCATTCAGTAGCGGAATTGATTTGGGAAGTATAC
CGCGATACGCAGTATCTAGATTACGTCGGCGGGATGCCTGGAGGCAAACAGAGACAGGCGAATCTCCGTGCTCTTTATGA
TCGAGCGAAGCAGTATGAAAAAGCTGCTTTTAGAGGGCTGTTCCGTTTTCTTCGCTTTATTGAAAGAATGCAGGAGCGTG
GAGATGATCTTGGGGCGGCAAAAACGTTCAGTGAAACAGAGGACGTTGTTCGCATGATGACGATCCATAGCAGTAAAGGA
TTAGAATTTCCAGTCGTCTTTACCGCAGGACTCGGCAGAAACTTTAATATGATGGACTTAAACCAGTCCTACTTACTTGA
TAAAGAATTAGGCTTTGGAAGCAAATATATTCATCCAGAGCTGAGAATTAGTTATGCAACATTACCGCTTGTTGCGATGA
AAAAAAAGATGAGGAAAGAGCTATTATCTGAAGAGCTGAGAGTTCTATATGTAGCGCTCACAAGGGCTAAGGAAAAATTA
TTTCTTGTCGGCTCTGTCAAAAATCAGGTGAAGGCATTAAGCAAATGGCAAAATGCTGCAACCGGAGAGGACTGGCTCCT
CCCTGATTTTGAACGTTATCAATCGAAAACATACTTAGATTTTATTGGTCCTGCTCTCATCCGTCACCAAGCAATGTCAT
CTATTTTAGAAGAAACAGGTGAAGTTGTTCTTTCGCACCCATCAGCATTCACAATCTCATTTACTCAAGCTTCCGATCTT
CTAAAAGAAGATATGTCACTTGAAAAGAAGCAGCAAGATGAGGTGGTACAAGCATTGATGGACGGCTTACCTGTTGAAGG
ATATGGTGATGCTGATAAGAAAGTAGCAGAGAGACTTTCATGGAGATACCCGTATCTAGCGACCTCACAAGTCGGCACAA
AGCAGTCTGTTTCAGAGATAAAGAGAATGAAAGAAATACAGGATGAGTACAGTGTTCCTTCTTCTCTTAGAAGAGCTCAT
GCAACGTTGTATGACCGGCCGGCTTTCATGAAGAAAAAAGCACTCACTGCCGCAGAGCAAGGCACCGCAATGCATACAGT
CATGCAGCATATTCCGCTTTCTTCTGACGAGCCCCTCGATGAAATCCATATACATCAGCTGCTCGATTCCTTACAACAGC
GAGACTTGCTAACAAATGAGCAAGTTCAGTCAATTGATCAAGAAGGAATTTCCGCTTTTTTCTCTACTTCTATTGGTCAA
AAGCTGCTGAAAGCGGACTGGGTGAAACGAGAAGTGTCATTTAGTATGGTGTTACCTGTTAAAGAGGTATACAGCCATAT
TGATGCAGAGGGGGAGCCTGTCCTAATTCAAGGGATGATTGACTGTTTATTTGAAACGGATGGAAAACTATATTTACTAG
ATTATAAAACAGATAGAGTGCAAGGCAGATATACAGGCGGTCTTGAAGCAGCTGTACCTATTTTGAAAAAGCGCTATGAA
ACTCAAATAGCGTTATATGCAAAAGCAGTAGAACGTTTAACAAACAGAACATTAGAGGAGAAAATTCTTTATTTCTTTGA
TGGGAATTTAGAAATTTCATTATAA

Domains

Predicted by InterproScan.

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(1052-1226)

UvrD/REP helicase N-terminal domain (UvrD-helicase)

(11-462)

UvrD-like helicase C-terminal domain (UvrD_C)

(473-883)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addA Bacillus subtilis subsp. subtilis str. 168

66.586

100

0.667

Multiple sequence alignment