Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   HGG68_RS00570 Genome accession   NZ_CP051263
Coordinates   122249..122689 (-) Length   146 a.a.
NCBI ID   WP_000360915.1    Uniprot ID   A0A0H2V436
Organism   Escherichia coli CFT073     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 117249..127689
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HGG68_RS00545 coaE 117739..118359 (-) 621 WP_001269525.1 dephospho-CoA kinase -
  HGG68_RS00550 - 118384..118428 (+) 45 WP_120795372.1 protein YacM -
  HGG68_RS00555 guaC 118584..119627 (+) 1044 WP_001217338.1 GMP reductase -
  HGG68_RS00560 hofC 119662..120864 (-) 1203 WP_000157242.1 protein transport protein HofC -
  HGG68_RS00565 pilB 120854..122239 (-) 1386 WP_001025180.1 type II secretion system protein GspE Machinery gene
  HGG68_RS00570 pilA 122249..122689 (-) 441 WP_000360915.1 prepilin peptidase-dependent pilin Machinery gene
  HGG68_RS00575 nadC 122893..123786 (-) 894 WP_001135168.1 carboxylating nicotinate-nucleotide diphosphorylase -
  HGG68_RS00580 ampD 123874..124425 (+) 552 WP_000923726.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  HGG68_RS00585 ampE 124422..125276 (+) 855 WP_000172013.1 beta-lactamase regulator AmpE -
  HGG68_RS00590 aroP 125319..126689 (-) 1371 WP_000399017.1 aromatic amino acid transporter AroP -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15607.77 Da        Isoelectric Point: 4.5237

>NTDB_id=383369 HGG68_RS00570 WP_000360915.1 122249..122689(-) (pilA) [Escherichia coli CFT073]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLNTCDGGSNGIPSPTTTR
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCTIQSDSALQQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=383369 HGG68_RS00570 WP_000360915.1 122249..122689(-) (pilA) [Escherichia coli CFT073]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAAATACCTGCGACGGTGGCAGCAATGGCATTCCTTCGCCTACCACCACCCGC
TATGTTTCAGCCATGAGTGTGGCAAAGGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGGCTAAGCGTCGTCAT
GACACCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGTAACTGCACTATTCAAAGTGACAGCGCATTGCAGC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0H2V436

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

43.089

84.247

0.363

Multiple sequence alignment