Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   HF527_RS01410 Genome accession   NZ_CP051219
Coordinates   306282..307775 (+) Length   497 a.a.
NCBI ID   WP_001040629.1    Uniprot ID   -
Organism   Escherichia coli strain SFE8     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 301282..312775
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HF527_RS01380 (HF527_01380) tusA 301529..301774 (+) 246 WP_000130621.1 sulfurtransferase TusA -
  HF527_RS01385 (HF527_01385) zntA 301876..304074 (-) 2199 WP_023155844.1 Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase ZntA -
  HF527_RS01390 (HF527_01390) yhhN 304148..304774 (-) 627 WP_000964718.1 lysoplasmalogenase -
  HF527_RS01395 (HF527_01395) yhhM 304915..305274 (+) 360 WP_000042901.1 DUF2500 domain-containing protein -
  HF527_RS01400 (HF527_01400) yhhL 305277..305546 (-) 270 WP_001295207.1 DUF1145 family protein -
  HF527_RS01405 (HF527_01405) rsmD 305536..306132 (-) 597 WP_000743193.1 16S rRNA (guanine(966)-N(2))-methyltransferase -
  HF527_RS01410 (HF527_01410) pilA 306282..307775 (+) 1494 WP_001040629.1 signal recognition particle-docking protein FtsY Machinery gene
  HF527_RS01415 (HF527_01415) ftsE 307778..308446 (+) 669 WP_000617723.1 cell division ATP-binding protein FtsE -
  HF527_RS01420 (HF527_01420) ftsX 308439..309497 (+) 1059 WP_001042003.1 permease-like cell division protein FtsX -
  HF527_RS01425 (HF527_01425) rpoH 309742..310596 (+) 855 WP_000130217.1 RNA polymerase sigma factor RpoH -
  HF527_RS01430 (HF527_01430) livJ 310867..311970 (+) 1104 WP_001021996.1 branched chain amino acid ABC transporter substrate-binding protein LivJ -
  HF527_RS01435 (HF527_01435) panM 312169..312552 (-) 384 WP_000778804.1 aspartate 1-decarboxylase autocleavage activator PanM -

Sequence

Protein


Download         Length: 497 a.a.        Molecular weight: 54544.36 Da        Isoelectric Point: 4.1560

>NTDB_id=383205 HF527_RS01410 WP_001040629.1 306282..307775(+) (pilA) [Escherichia coli strain SFE8]
MAKEKKRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVQAQEPAKASEQAVEEQPQAHTEAEAETFAADVVEVTEQVAE
SEKAQPEAEVVAQPEPVVEETAEPVAIEREELPLPEDVNAEEVSPEEWQAEAETVEIVEAAEEEAAKEEITDEELEAQAL
AAEAAEEAVMVVPPVEEQPVEEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISLFRGKKIDDDLFEELEEQLLIADV
GVETTRKIITNLTEGASRKQLRDAEALYGLLKEEMGEILAKVDEPLNVEGKTPFVILMVGVNGVGKTTTIGKLARQFEQQ
GKSVMLAAGDTFRAAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKSHLMEELKKI
VRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLDGTAKGGVIFSVADQFGIPIRYIGVGERIEDL
RPFKADDFIEALFARED

Nucleotide


Download         Length: 1494 bp        

>NTDB_id=383205 HF527_RS01410 WP_001040629.1 306282..307775(+) (pilA) [Escherichia coli strain SFE8]
ATGGCGAAAGAAAAAAAACGTGGCTTTTTTTCCTGGCTGGGCTTTGGTCAAAAAGAGCAGACCCCGGAAAAAGAGACAGA
AGTTCAGAATGAACAACCGGTTGTAGAAGAAATCGTTCAGGCGCAAGAGCCTGCGAAGGCTTCTGAACAAGCCGTCGAAG
AGCAACCGCAGGCGCATACTGAAGCCGAGGCGGAAACTTTTGCTGCCGACGTTGTGGAAGTCACTGAACAGGTTGCTGAA
AGTGAAAAAGCGCAGCCTGAAGCGGAAGTCGTTGCACAGCCGGAACCGGTCGTAGAAGAAACGGCGGAGCCAGTGGCTAT
CGAACGTGAAGAGCTGCCGTTGCCGGAAGACGTCAACGCCGAAGAGGTTTCGCCAGAAGAGTGGCAGGCTGAAGCGGAAA
CCGTAGAGATTGTCGAAGCGGCGGAAGAAGAAGCGGCTAAAGAAGAAATCACCGACGAAGAGCTGGAAGCACAGGCGCTG
GCTGCCGAAGCGGCAGAAGAGGCGGTGATGGTGGTTCCTCCGGTAGAAGAGCAGCCGGTGGAAGAAATCGCTCAGGAGCA
GGAAAAACCGACCAAAGAAGGTTTCTTCGCGCGCCTGAAACGCAGCCTGTTAAAAACCAAAGAAAATCTCGGTTCCGGAT
TTATCAGCCTGTTCCGCGGTAAAAAAATCGACGATGATCTGTTTGAAGAGCTGGAAGAACAGCTGTTGATCGCCGATGTG
GGCGTGGAAACCACACGTAAAATCATCACCAATCTGACGGAAGGCGCATCCCGCAAGCAGCTTCGTGATGCCGAGGCCCT
CTATGGCCTGCTGAAAGAAGAGATGGGCGAGATTCTGGCGAAAGTCGATGAGCCGCTGAATGTTGAAGGTAAAACGCCGT
TCGTGATCCTGATGGTAGGCGTCAACGGTGTGGGTAAAACCACGACGATTGGTAAGCTGGCGCGTCAGTTTGAGCAGCAG
GGTAAATCGGTGATGCTGGCGGCGGGCGATACTTTCCGTGCAGCAGCGGTTGAACAGCTTCAGGTCTGGGGGCAGCGCAA
CAATATTCCGGTGATTGCCCAGCATACCGGTGCGGATTCCGCCTCTGTTATCTTCGACGCCATTCAGGCCGCTAAAGCGC
GTAATATCGACGTCCTGATTGCCGATACCGCCGGACGCCTGCAGAACAAATCGCACCTGATGGAAGAGTTGAAGAAAATC
GTCCGCGTGATGAAGAAACTCGACGTTGAAGCGCCGCATGAAGTTATGCTGACTATCGATGCCAGCACCGGGCAGAACGC
GGTAAGCCAGGCCAAACTGTTCCATGAAGCTGTTGGCTTAACCGGCATCACGCTAACGAAACTGGACGGCACGGCGAAAG
GCGGGGTAATTTTCTCGGTGGCTGACCAGTTTGGTATCCCTATCCGCTACATTGGTGTCGGCGAACGTATTGAGGATTTG
CGTCCGTTTAAGGCGGACGACTTTATAGAGGCACTTTTTGCCCGAGAGGATTAA

Domains

Predicted by InterProScan.

SRP54-type protein, helical bundle domain (SRP54_N)

(210-275)

SRP54-type protein, GTPase domain (SRP54)

(293-493)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Neisseria gonorrhoeae MS11

47.901

81.489

0.39

Multiple sequence alignment