Detailed information NTG0383203

Bioinformatically predicted

Overview

• Name: amiE
• Type: Regulator
• Locus tag: HF527_RS01005
• Genome accession: NZ_CP051219
• Coordinates: 211413..212396 (+)
• Length: 327 a.a.
• NCBI ID: WP_001196486.1
• Uniprot ID: A7ZT85
• Organism: Escherichia coli strain SFE8
• Function: internalize XIP (predicted from homology) Competence regulation

Genomic Context

Location: 206413..217396

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
HF527_RS00990 (HF527_00990)	dppA	207713..209320 (+)	1608	WP_001222876.1	dipeptide ABC transporter substrate-binding protein DppA	-
HF527_RS00995 (HF527_00995)	dppB	209471..210490 (+)	1020	WP_000938855.1	dipeptide ABC transporter permease DppB	-
HF527_RS01000 (HF527_01000)	dppC	210500..211402 (+)	903	WP_000084677.1	dipeptide ABC transporter permease DppC	-
HF527_RS01005 (HF527_01005)	amiE	211413..212396 (+)	984	WP_001196486.1	dipeptide ABC transporter ATP-binding protein	Regulator
HF527_RS01010 (HF527_01010)	dppF	212393..213397 (+)	1005	WP_000107030.1	dipeptide ABC transporter ATP-binding subunit DppF	-
HF527_RS01015 (HF527_01015)	yhjV	213427..214698 (-)	1272	WP_001402620.1	aromatic amino acid transport family protein	-
HF527_RS01020 (HF527_01020)	ldrD	215174..215281 (+)	108	WP_000141634.1	type I toxin-antitoxin system toxic polypeptide LdrD	-
HF527_RS01025 (HF527_01025)	-	215657..215764 (+)	108	WP_001295224.1	type I toxin-antitoxin system toxin Ldr family protein	-
HF527_RS01030 (HF527_01030)	-	216140..216247 (+)	108	WP_001295224.1	type I toxin-antitoxin system toxin Ldr family protein	-

Sequence

Protein

• Length: 327 a.a.
• Molecular weight: 35844.36 Da
• Isoelectric Point: 6.5814

>NTDB_id=383203 HF527_RS01005 WP_001196486.1 211413..212396(+) (amiE) [Escherichia coli strain SFE8]
MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGSGKSVSSLAIMGLIDYPGRVMAEKLEFNGQDLQRISE
KERRNLVGAEVAMIFQDPMTSLNPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNQVGIPDPASRLDVYPHQLSGGMSQ
RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVAEAAHKIIVMYAGQVVETGDAHA
IFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPGKYDRPNGCLLNPRCPYATDRCRAEEPALNMLADGRQSKCHYPLD
DAGRPTL

Nucleotide

• Length: 984 bp

>NTDB_id=383203 HF527_RS01005 WP_001196486.1 211413..212396(+) (amiE) [Escherichia coli strain SFE8]
ATGGCGTTATTAAATGTAGATAAATTATCGGTGCATTTCGGCGACGAAAGCGCGCCGTTCCGCGCCGTAGACCGCATCAG
CTACAGCGTAAAACAGGGCGAAGTGGTCGGGATTGTGGGTGAGTCCGGCTCCGGTAAGTCGGTCAGTTCACTGGCGATTA
TGGGGCTGATTGATTATCCGGGCCGCGTAATGGCAGAAAAACTGGAGTTTAACGGCCAGGATTTGCAGCGTATCTCAGAA
AAAGAGCGCCGCAACCTGGTGGGTGCCGAAGTGGCGATGATCTTCCAGGACCCGATGACCAGCCTTAACCCGTGCTACAC
CGTGGGTTTCCAGATTATGGAAGCGATTAAGGTGCATCAGGGCGGTAATAAGAGCACTCGCCGTCAGCGAGCGATTGACC
TGCTGAATCAGGTCGGTATTCCCGATCCGGCATCGCGTCTGGATGTTTACCCGCATCAGCTTTCTGGCGGTATGAGTCAG
CGTGTGATGATCGCCATGGCGATTGCCTGTCGGCCAAAACTGCTGATTGCCGATGAACCAACCACTGCGCTGGACGTGAC
CATTCAGGCGCAAATCATTGAACTACTGCTGGAGTTACAGCAGAAAGAGAACATGGCGCTGGTGTTAATTACCCATGACC
TGGCGCTGGTGGCGGAAGCGGCACATAAAATCATCGTGATGTATGCCGGTCAGGTGGTGGAAACGGGCGATGCGCACGCC
ATCTTCCATGCGCCGCGTCACCCGTATACTCAGGCATTGCTGCGTGCGCTGCCAGAATTTGCTCAGGACAAAGAACGTCT
GGCGTCGTTGCCAGGTGTTGTTCCCGGTAAGTACGACCGCCCGAACGGCTGCCTGCTTAACCCGCGCTGCCCCTATGCCA
CTGACAGATGTCGCGCTGAAGAACCGGCGCTGAATATGCTCGCTGATGGGCGTCAGTCCAAATGCCATTACCCACTTGAT
GATGCCGGGAGGCCGACACTATGA

Domains

Predicted by InterProScan.

• Oligopeptide/dipeptide transporter, C-terminal region (oligo_HPY) (233-297)

• ABC transporter (ABC_tran) (24-181)

3D structure

• AlphaFold DB: A7ZT85

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
amiE	Streptococcus salivarius strain HSISS4	43.302	98.165	0.425
amiE	Streptococcus thermophilus LMG 18311	42.991	98.165	0.422
amiE	Streptococcus thermophilus LMD-9	42.991	98.165	0.422
oppD	Streptococcus mutans UA159	41.379	97.554	0.404