Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   EKN98_RS14215 Genome accession   NZ_CP043382
Coordinates   2909213..2910646 (-) Length   477 a.a.
NCBI ID   WP_003861864.1    Uniprot ID   -
Organism   Enterobacter hormaechei subsp. xiangfangensis strain WCHEX045001     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2904213..2915646
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EKN98_RS14185 (EKN98_014185) elbB 2905745..2906398 (+) 654 WP_023314910.1 isoprenoid biosynthesis glyoxalase ElbB -
  EKN98_RS14190 (EKN98_014190) mtgA 2906395..2907120 (+) 726 WP_003861874.1 monofunctional biosynthetic peptidoglycan transglycosylase -
  EKN98_RS14195 (EKN98_014195) npr 2907160..2907432 (-) 273 WP_003861872.1 PTS phosphocarrier protein NPr -
  EKN98_RS14200 (EKN98_014200) rapZ 2907429..2908283 (-) 855 WP_003861870.1 RNase adapter RapZ -
  EKN98_RS14205 (EKN98_014205) ptsN 2908329..2908820 (-) 492 WP_003861869.1 PTS IIA-like nitrogen regulatory protein PtsN -
  EKN98_RS14210 (EKN98_014210) hpf 2908903..2909190 (-) 288 WP_003861866.1 ribosome hibernation promoting factor -
  EKN98_RS14215 (EKN98_014215) rpoN/rpoN1 2909213..2910646 (-) 1434 WP_003861864.1 RNA polymerase factor sigma-54 Machinery gene
  EKN98_RS14220 (EKN98_014220) lptB 2910694..2911419 (-) 726 WP_003861862.1 LPS export ABC transporter ATP-binding protein -
  EKN98_RS14225 (EKN98_014225) lptA 2911426..2911980 (-) 555 WP_003861860.1 lipopolysaccharide ABC transporter substrate-binding protein LptA -
  EKN98_RS14230 (EKN98_014230) lptC 2911949..2912524 (-) 576 WP_003861858.1 LPS export ABC transporter periplasmic protein LptC -
  EKN98_RS14235 (EKN98_014235) kdsC 2912521..2913087 (-) 567 WP_003861856.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  EKN98_RS14240 (EKN98_014240) kdsD 2913102..2914088 (-) 987 WP_003861853.1 arabinose-5-phosphate isomerase KdsD -
  EKN98_RS14245 (EKN98_014245) - 2914102..2915079 (-) 978 WP_003861852.1 calcium/sodium antiporter -

Sequence

Protein


Download         Length: 477 a.a.        Molecular weight: 54099.96 Da        Isoelectric Point: 4.3358

>NTDB_id=383139 EKN98_RS14215 WP_003861864.1 2909213..2910646(-) (rpoN/rpoN1) [Enterobacter hormaechei subsp. xiangfangensis strain WCHEX045001]
MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALDSNPLLEQTDLHDEVDAQQTQDTETLDSVDALEQKEMPD
ELPLDASWDEIYTAGTPSGTRADYQDDELPVYQGETTQSLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVTLDE
ILESIGDDEIELEEIEAVLKRVQRFDPIGVAAKDLRDCLLIQLSQFAKETPWIDEARLIISDHLDLLANHDFRTLMRVTR
LKEEVLKEAVNLIQSLDPRPGQSIQTSEPEYVIPDVLVRKHNGRWVVELNADSIPRLQINQQYASMCTSARNDADNQYIR
SNLQEARWLIKSLESRNDTLLRVSRCIVEQQQAFFEQGEEFMKPMVLADIAQAVEMHESTISRVTTQKYLHSPRGIFELK
YFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTTMLSDQGIMVARRTVAKYRESLSIPPSNQRKQLV

Nucleotide


Download         Length: 1434 bp        

>NTDB_id=383139 EKN98_RS14215 WP_003861864.1 2909213..2910646(-) (rpoN/rpoN1) [Enterobacter hormaechei subsp. xiangfangensis strain WCHEX045001]
ATGAAGCAAGGTTTGCAATTACGGCTCAGCCAGCAACTGGCCATGACGCCGCAGCTCCAGCAGGCAATCCGTCTGTTGCA
GCTGTCCACGTTAGAACTCCAGCAGGAGCTCCAGCAGGCGCTGGACAGCAATCCGCTGCTGGAGCAGACCGATCTTCATG
ACGAGGTAGACGCCCAGCAGACGCAGGACACAGAAACGCTCGACTCCGTAGATGCACTCGAACAGAAAGAGATGCCGGAC
GAACTGCCGCTGGATGCCAGCTGGGATGAAATCTACACCGCGGGTACCCCTTCCGGGACGCGTGCAGACTACCAGGACGA
TGAACTCCCGGTCTACCAGGGCGAAACTACCCAATCGCTTCAGGACTACCTGATGTGGCAGGTGGAGCTGACGCCTTTCT
CCGATACCGATCGCGCCATTGCCACCTCGATTGTCGATGCCGTTGACGACACGGGCTATTTGACCGTGACGCTGGACGAG
ATTCTTGAAAGCATCGGCGATGACGAGATTGAACTGGAAGAGATCGAAGCCGTCCTGAAACGCGTTCAGCGTTTCGACCC
GATTGGCGTCGCCGCGAAAGATCTGCGTGACTGCCTTCTGATCCAGCTTTCGCAGTTTGCTAAAGAGACACCGTGGATTG
ACGAAGCCCGGCTCATCATCAGCGATCATCTGGATCTGCTGGCGAACCACGACTTCCGCACCCTGATGCGCGTCACGCGC
CTGAAAGAAGAGGTGCTGAAGGAAGCGGTGAATCTGATCCAGTCGCTCGATCCGCGTCCGGGGCAGTCGATTCAGACCAG
CGAACCGGAATATGTCATTCCCGATGTGCTGGTGAGAAAACACAATGGCCGCTGGGTCGTTGAACTGAACGCCGACAGCA
TTCCTCGCCTGCAAATCAACCAGCAATACGCCTCCATGTGCACCAGCGCCCGTAACGACGCTGATAATCAATACATTCGC
AGCAACCTTCAGGAAGCACGCTGGTTGATTAAGAGTCTGGAGAGCCGTAATGACACGCTGCTGCGCGTCAGCCGCTGTAT
TGTCGAACAGCAGCAGGCATTCTTTGAGCAGGGCGAAGAGTTTATGAAACCGATGGTGCTGGCGGACATAGCCCAGGCCG
TCGAGATGCATGAATCAACCATTTCCCGCGTGACCACGCAGAAGTATCTGCACAGCCCACGCGGTATTTTTGAGCTTAAG
TATTTCTTCTCCAGCCACGTGAACACCGAAGGCGGCGGCGAAGCCTCGTCGACGGCCATTCGCGCACTGGTGAAGAAGTT
GATCGCCGCGGAGAACCCCGCGAAGCCACTCAGTGACAGTAAGTTAACCACCATGCTGTCCGATCAGGGTATTATGGTGG
CACGTCGTACTGTCGCGAAGTATCGAGAGTCTTTATCCATTCCGCCGTCTAACCAGCGTAAACAACTGGTTTGA

Domains

Predicted by InterproScan.

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-303)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(317-475)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

45.347

100

0.48

Multiple sequence alignment