Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   D5067_RS02605 Genome accession   NZ_CP043342
Coordinates   536790..538223 (-) Length   477 a.a.
NCBI ID   WP_119936817.1    Uniprot ID   A0A3R9VXX8
Organism   Enterobacter huaxiensis strain 090008     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 531790..543223
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  D5067_RS02575 (D5067_0002595) elbB 533324..533977 (+) 654 WP_119936812.1 isoprenoid biosynthesis glyoxalase ElbB -
  D5067_RS02580 (D5067_0002600) mtgA 533974..534699 (+) 726 WP_119936813.1 monofunctional biosynthetic peptidoglycan transglycosylase -
  D5067_RS02585 (D5067_0002605) npr 534737..535009 (-) 273 WP_003861872.1 PTS phosphocarrier protein NPr -
  D5067_RS02590 (D5067_0002610) rapZ 535006..535860 (-) 855 WP_119936814.1 RNase adapter RapZ -
  D5067_RS02595 (D5067_0002615) ptsN 535906..536397 (-) 492 WP_119936815.1 PTS IIA-like nitrogen regulatory protein PtsN -
  D5067_RS02600 (D5067_0002620) hpf 536480..536767 (-) 288 WP_119936816.1 ribosome hibernation promoting factor -
  D5067_RS02605 (D5067_0002625) rpoN/rpoN1 536790..538223 (-) 1434 WP_119936817.1 RNA polymerase factor sigma-54 Machinery gene
  D5067_RS02610 (D5067_0002630) lptB 538271..538996 (-) 726 WP_119936818.1 LPS export ABC transporter ATP-binding protein -
  D5067_RS02615 (D5067_0002635) lptA 539003..539557 (-) 555 WP_119936819.1 lipopolysaccharide ABC transporter substrate-binding protein LptA -
  D5067_RS02620 (D5067_0002640) lptC 539526..540101 (-) 576 WP_119936820.1 LPS export ABC transporter periplasmic protein LptC -
  D5067_RS02625 (D5067_0002645) kdsC 540098..540664 (-) 567 WP_119936821.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  D5067_RS02630 (D5067_0002650) kdsD 540679..541665 (-) 987 WP_119936822.1 arabinose-5-phosphate isomerase KdsD -
  D5067_RS02635 (D5067_0002655) - 541679..542656 (-) 978 WP_119936823.1 calcium/sodium antiporter -

Sequence

Protein


Download         Length: 477 a.a.        Molecular weight: 54090.91 Da        Isoelectric Point: 4.3358

>NTDB_id=382964 D5067_RS02605 WP_119936817.1 536790..538223(-) (rpoN/rpoN1) [Enterobacter huaxiensis strain 090008]
MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALDSNPLLEQTDLHDEVDTQETQDTETLDSVDALEQKEMPD
ELPLDASWDEIYTAGTPSGTRADYQDDELPVYQGETTQSLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVTLDD
ILESIGNDEIELEEIEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFAKETPWIDEARVIISSHLDLLANHDFRTLMRVTR
LKEEVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDVLVRKHNDRWVVELNSDSIPRLQINQQYASMCTSARNDADNQYIR
SNLQEARWLIKSLESRNDTLLRVSRCIVEQQQAFFEQGEEFMKPMVLADIAQAVEMHESTISRVTTQKYLHSPRGIFELK
YFFSSHVSTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTTMLSDQGIMVARRTVAKYRESLSIPPSNQRKQLV

Nucleotide


Download         Length: 1434 bp        

>NTDB_id=382964 D5067_RS02605 WP_119936817.1 536790..538223(-) (rpoN/rpoN1) [Enterobacter huaxiensis strain 090008]
ATGAAGCAAGGTTTGCAATTAAGGCTCAGCCAACAACTGGCAATGACGCCGCAGCTACAGCAGGCGATCCGTCTGCTGCA
GCTGTCCACGTTAGAACTCCAGCAGGAGCTCCAGCAGGCGCTGGACAGTAATCCCCTGCTGGAGCAAACCGATCTTCATG
ACGAGGTAGATACCCAGGAAACACAAGACACTGAAACGCTCGACTCCGTTGATGCACTCGAACAAAAAGAGATGCCGGAT
GAGCTTCCGCTTGATGCCAGCTGGGATGAAATCTACACCGCCGGGACCCCTTCCGGCACGCGTGCAGACTACCAGGACGA
CGAGCTGCCGGTCTATCAGGGAGAAACCACCCAGTCGCTGCAGGATTACCTGATGTGGCAGGTTGAACTGACTCCCTTCT
CCGATACCGATCGCGCTATTGCGACCTCCATTGTCGATGCCGTGGATGACACCGGCTATCTGACCGTCACGCTGGACGAC
ATTCTGGAAAGCATCGGCAACGATGAGATCGAACTCGAAGAGATCGAGGCCGTGCTGAAGCGCATTCAGCGTTTCGACCC
GGTCGGCGTGGCCGCAAAAGATCTCCGCGACTGCCTGCTGATCCAGCTGTCTCAGTTTGCCAAAGAGACGCCGTGGATTG
ACGAAGCCCGCGTGATCATCAGCAGCCATCTCGATCTGCTCGCAAACCACGATTTTCGCACCCTGATGCGCGTCACGCGC
CTGAAGGAAGAGGTGCTGAAAGAAGCGGTTAACCTGATCCAATCCCTCGATCCGCGCCCCGGCCAGTCGATCCAGACCGG
CGAACCCGAATATGTGATCCCCGATGTGCTGGTGCGAAAACATAACGACCGCTGGGTCGTAGAGCTGAATTCTGACAGCA
TCCCTCGCCTGCAAATCAACCAGCAGTATGCCTCCATGTGCACCAGCGCGCGTAATGACGCCGACAATCAGTACATTCGC
AGCAATCTGCAGGAAGCACGCTGGTTGATCAAAAGTCTGGAGAGCCGAAATGATACGCTGCTGCGCGTAAGCCGCTGTAT
TGTCGAGCAGCAGCAGGCTTTCTTTGAGCAGGGCGAAGAGTTTATGAAACCGATGGTACTGGCGGATATCGCCCAGGCCG
TCGAGATGCACGAATCAACCATTTCTCGCGTGACCACGCAGAAGTATCTGCACAGTCCGCGCGGTATTTTTGAGCTTAAG
TATTTCTTCTCAAGCCATGTGAGTACCGAGGGCGGAGGCGAAGCCTCGTCGACGGCAATCCGTGCGCTGGTGAAGAAGTT
GATCGCCGCGGAAAACCCCGCGAAGCCACTGAGTGACAGTAAGTTAACCACCATGCTGTCCGACCAGGGTATTATGGTGG
CACGTCGTACTGTTGCGAAGTATCGAGAGTCTTTATCCATTCCGCCGTCTAACCAGCGTAAACAGCTGGTCTGA

Domains

Predicted by InterproScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-303)

Sigma-54, DNA binding domain (Sigma54_DBD)

(317-475)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A3R9VXX8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

45.347

100

0.48

Multiple sequence alignment