Detailed information    

insolico Bioinformatically predicted

Overview

Name   recJ   Type   Machinery gene
Locus tag   HF584_RS01035 Genome accession   NZ_CP051165
Coordinates   215990..218263 (+) Length   757 a.a.
NCBI ID   WP_054190073.1    Uniprot ID   -
Organism   Staphylococcus aureus strain NGA84b     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 210990..223263
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HF584_RS01020 (HF584_07835) tgt 211815..212954 (+) 1140 WP_054190075.1 tRNA guanosine(34) transglycosylase Tgt -
  HF584_RS01025 (HF584_07830) yajC 212973..213233 (+) 261 WP_001160682.1 preprotein translocase subunit YajC -
  HF584_RS01030 (HF584_07825) secDF 213508..215787 (+) 2280 WP_054190074.1 protein translocase subunit SecDF -
  HF584_RS01035 (HF584_07820) recJ 215990..218263 (+) 2274 WP_054190073.1 single-stranded-DNA-specific exonuclease RecJ Machinery gene
  HF584_RS01040 (HF584_07815) - 218285..218803 (+) 519 WP_054190072.1 adenine phosphoribosyltransferase -
  HF584_RS01045 (HF584_07810) - 219230..221419 (+) 2190 WP_070016694.1 bifunctional (p)ppGpp synthetase/guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase -
  HF584_RS01050 (HF584_07805) dtd 221431..221883 (+) 453 WP_000869979.1 D-aminoacyl-tRNA deacylase -
  HF584_RS01055 (HF584_07800) - 221880..222755 (+) 876 WP_054190071.1 N-acetylmuramoyl-L-alanine amidase -

Sequence

Protein


Download         Length: 757 a.a.        Molecular weight: 85550.90 Da        Isoelectric Point: 5.0213

>NTDB_id=382840 HF584_RS01035 WP_054190073.1 215990..218263(+) (recJ) [Staphylococcus aureus strain NGA84b]
MIKPKYKWKLIKPTEYISDELTSKLKLTPIVKKILESKSIIDEQAIESIISDTDINHDALQLSDMTKAIDRIKRAIANDE
KILVYGDYDADGVTSTTILVTTLQLLGAQVGWHIPNRFTEGYGPNELAFRNAHDEGITLIITVDNGIQGHNEIKMVQDLG
VDVIVTDHHEIGSTLPEAYAIVHPMHPSFNYPFQQLCGAGVAYKLAQALIENVPDYFKALVAIGTIADLVSLTDENRSLV
KQGLKVLNDQCPTSVKALLKEAGYNDNIDEETIGFIIGPRLNAVGRLDDASLACELLMTDDEEEAAFLAEQVEHFNRERK
DIVATITEEAMAMAETKVKNGDLFLLLAKENWHEGVLGIVASKIVETFALPTLILNVDREQNHAKGSARSIDQVSMFEIL
SAHQELIAKFGGHHMAAGMTMDIENIEALAEGLNKWMKELSKTTSLDPVKPVDVLLTENDITIKNIRDMNRLRPFGTDFS
RPIFEMDDLSVSSVKAIGQQKNHLKLTLGESNIAALFWQNGHLEPELQNEQPINILGSVQINEWNGNQSPQLIIQDIAMN
EQQILDYRSKRKSLPFTENDKNIVVLIHPKSDKVNANEYYYGEEIKQQTDKVVLRDLPTSMKDLSNSLQQLQFSQLYIVL
QHNHSIYFDGIPNMDVFKKCYKALITKQETNIQKEGMLLCQHLSVKPDTLKFMLKVFLDLKFVTQEDGLIRINQQPEKRS
IDSSKVYQLRQQRMDVEKQLLYQDFSEIKNWIKSQLS

Nucleotide


Download         Length: 2274 bp        

>NTDB_id=382840 HF584_RS01035 WP_054190073.1 215990..218263(+) (recJ) [Staphylococcus aureus strain NGA84b]
ATGATTAAACCGAAGTATAAGTGGAAATTAATTAAACCAACTGAATACATAAGTGATGAATTAACATCAAAATTAAAGTT
AACGCCAATCGTAAAAAAAATATTAGAAAGTAAATCGATTATTGATGAACAAGCAATAGAATCCATCATTTCAGATACAG
ATATCAATCATGATGCACTGCAATTGAGCGATATGACTAAGGCTATTGATAGAATTAAAAGAGCGATAGCGAATGATGAG
AAAATCCTAGTTTATGGTGATTACGATGCCGACGGTGTTACCTCAACTACAATTTTAGTGACAACATTACAACTTTTAGG
TGCACAAGTAGGATGGCATATTCCTAACCGCTTTACCGAAGGATATGGACCAAACGAACTAGCATTTAGAAATGCTCATG
ATGAAGGCATAACATTAATCATAACTGTTGATAATGGCATACAAGGCCATAATGAAATTAAAATGGTTCAAGATTTAGGA
GTTGATGTCATTGTAACTGATCATCATGAAATTGGTAGTACATTACCAGAAGCATATGCAATCGTACATCCAATGCATCC
ATCGTTTAATTATCCGTTTCAACAATTGTGTGGTGCAGGTGTTGCGTACAAATTAGCACAAGCATTGATTGAAAATGTAC
CTGATTATTTTAAAGCATTAGTTGCGATAGGTACCATTGCGGATTTAGTATCCTTAACTGATGAAAATAGATCACTAGTG
AAACAAGGATTAAAAGTCTTAAATGATCAATGTCCGACCTCTGTTAAAGCATTGCTAAAAGAAGCGGGATACAATGACAA
TATTGATGAAGAAACAATTGGTTTTATTATTGGACCTAGACTTAATGCTGTAGGTCGTTTAGATGACGCATCATTAGCTT
GTGAATTGTTGATGACTGACGACGAAGAAGAAGCTGCATTTTTAGCAGAACAAGTTGAACACTTTAATCGCGAAAGAAAA
GATATAGTAGCAACTATCACAGAAGAAGCAATGGCAATGGCAGAAACGAAAGTTAAAAATGGAGATTTATTTTTACTTTT
AGCAAAAGAAAATTGGCATGAAGGCGTCCTAGGTATTGTTGCTTCGAAAATTGTCGAAACATTTGCTTTGCCAACATTAA
TTTTAAATGTTGATAGGGAGCAAAATCATGCAAAAGGTTCCGCCAGATCAATTGATCAAGTGTCGATGTTTGAAATACTA
TCAGCACACCAAGAGTTAATCGCTAAATTTGGCGGTCATCATATGGCTGCAGGTATGACGATGGATATTGAAAATATAGA
AGCGTTAGCTGAAGGTTTAAATAAATGGATGAAAGAATTGTCTAAAACAACTTCATTAGATCCGGTTAAGCCAGTAGATG
TATTATTAACAGAAAATGATATAACGATTAAAAATATACGTGATATGAATCGTTTAAGACCTTTCGGAACAGATTTTTCT
AGACCTATTTTTGAAATGGATGATTTATCAGTTTCTTCAGTTAAGGCAATAGGTCAACAAAAAAATCATCTAAAATTGAC
ACTTGGTGAAAGTAATATCGCCGCGCTATTTTGGCAAAATGGACACTTAGAACCTGAGTTACAAAATGAACAGCCAATTA
ATATATTAGGTTCTGTTCAAATCAACGAATGGAATGGTAATCAATCACCGCAATTAATTATTCAAGATATTGCGATGAAC
GAACAGCAAATATTAGATTATAGAAGTAAGCGGAAAAGTTTACCTTTTACAGAAAATGATAAAAATATTGTCGTGCTTAT
TCATCCTAAAAGTGATAAAGTAAATGCGAATGAATATTATTATGGTGAAGAAATTAAACAACAAACTGATAAAGTAGTAT
TAAGAGATTTACCTACGTCAATGAAAGATTTGTCTAATTCCCTGCAACAACTGCAATTTTCTCAACTTTATATAGTTTTG
CAACATAATCATTCGATTTACTTCGATGGTATACCTAATATGGATGTTTTTAAAAAGTGTTATAAAGCATTAATAACTAA
ACAAGAAACAAATATCCAGAAAGAGGGTATGTTATTGTGTCAACATTTAAGTGTGAAACCAGATACACTTAAATTCATGT
TGAAAGTTTTCTTAGACTTAAAATTTGTAACACAAGAAGATGGTTTAATTCGAATCAATCAACAACCTGAAAAAAGATCG
ATTGATTCCAGTAAAGTATATCAATTAAGACAACAACGTATGGATGTTGAAAAGCAATTATTATATCAAGATTTTTCAGA
AATAAAAAATTGGATAAAGTCACAATTGTCGTGA

Domains

Predicted by InterProScan.

DHH family (DHH)

(81-224)

RecJ OB domain (RecJ_OB)

(452-556)

Single-strand DNA-specific exonuclease, C terminal domain (ssDNA-exonuc_C)

(563-752)

DHHA1 domain (DHHA1)

(312-436)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recJ Bacillus subtilis subsp. subtilis str. 168

38.015

100

0.39
  recJ Streptococcus pneumoniae strain D39V

37.895

100

0.38