Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   FYN22_RS02385 Genome accession   NZ_CP043307
Coordinates   493404..494204 (+) Length   266 a.a.
NCBI ID   WP_062031315.1    Uniprot ID   A0AAJ6LDX5
Organism   Acinetobacter johnsonii strain Acsw19     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 488404..499204
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FYN22_RS02355 (FYN22_02355) - 488614..489030 (+) 417 WP_004983505.1 hypothetical protein -
  FYN22_RS02360 (FYN22_02360) - 489171..490451 (+) 1281 WP_148825481.1 glycosyltransferase family 4 protein -
  FYN22_RS02365 (FYN22_02365) - 490644..491195 (+) 552 WP_148825482.1 phosphatase PAP2 family protein -
  FYN22_RS02370 (FYN22_02370) iscX 491286..491486 (+) 201 WP_004697668.1 Fe-S cluster assembly protein IscX -
  FYN22_RS02375 (FYN22_02375) ndk 491597..492028 (+) 432 WP_004983508.1 nucleoside-diphosphate kinase -
  FYN22_RS02380 (FYN22_02380) rlmN 492152..493387 (+) 1236 WP_004697671.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  FYN22_RS02385 (FYN22_02385) pilF 493404..494204 (+) 801 WP_062031315.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  FYN22_RS02390 (FYN22_02390) - 494195..494971 (+) 777 WP_062031311.1 helix-turn-helix domain-containing protein -
  FYN22_RS02395 (FYN22_02395) ispG 494990..496105 (+) 1116 WP_004697677.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  FYN22_RS02400 (FYN22_02400) hisS 496102..497397 (+) 1296 WP_062031308.1 histidine--tRNA ligase -
  FYN22_RS02405 (FYN22_02405) - 497425..498123 (+) 699 WP_148825483.1 YfgM family protein -

Sequence

Protein


Download         Length: 266 a.a.        Molecular weight: 30076.05 Da        Isoelectric Point: 9.1263

>NTDB_id=382759 FYN22_RS02385 WP_062031315.1 493404..494204(+) (pilF) [Acinetobacter johnsonii strain Acsw19]
MKKPVSKLYLALTLGVSVWYLVGCQTVNTKGDPSKAVQVRTQLAAEYIKSNDYDAAKRTLDQALDIDSRDASANMMMGVL
LQREGSPQNVEKAERYFKHAIAAEPKNAQARNNYGTYLYQIGRYNDAIDQLQVAGSTLGYDQRYRALENLGRAYIQVGRV
AEAEAAFKQALQVNSGAYLAMIEMAEISYLRQQNAEATQYYEQFVRAVGEKNLDARALWIGIRIARANHDTMGSQVLVNQ
LRALYPDSQEYKRYLQLQYTTEAVWK

Nucleotide


Download         Length: 801 bp        

>NTDB_id=382759 FYN22_RS02385 WP_062031315.1 493404..494204(+) (pilF) [Acinetobacter johnsonii strain Acsw19]
TTGAAAAAACCCGTTTCTAAACTTTACCTTGCGCTGACACTGGGTGTGTCAGTGTGGTACTTGGTGGGTTGCCAAACCGT
GAATACGAAAGGTGATCCAAGTAAAGCAGTACAAGTTAGAACGCAATTGGCGGCTGAATATATTAAGTCGAATGATTACG
ATGCTGCGAAACGCACATTGGATCAGGCATTAGACATCGACTCTCGTGATGCATCTGCAAATATGATGATGGGCGTATTG
TTACAGCGTGAAGGCAGTCCGCAAAATGTGGAAAAGGCAGAGCGTTATTTTAAGCATGCAATTGCGGCTGAGCCAAAAAA
TGCACAGGCTCGTAACAACTACGGTACGTATTTATATCAAATTGGGCGTTATAATGACGCGATTGATCAACTTCAAGTTG
CAGGTTCAACTTTAGGGTATGATCAACGTTATCGAGCATTGGAAAATTTAGGTCGTGCGTATATACAAGTAGGTCGAGTG
GCGGAAGCAGAAGCTGCTTTTAAACAAGCCCTACAGGTAAACTCAGGTGCCTATCTTGCCATGATCGAAATGGCGGAAAT
TTCATATTTACGTCAGCAAAATGCTGAAGCTACGCAGTACTATGAACAGTTTGTGCGAGCGGTTGGCGAGAAAAACTTAG
ATGCACGTGCGCTTTGGATTGGGATCCGAATTGCGCGAGCAAATCATGACACGATGGGTTCGCAAGTACTGGTCAACCAG
CTACGTGCATTGTATCCAGACAGTCAAGAATACAAACGTTATTTGCAATTACAGTACACTACTGAGGCCGTATGGAAGTA
A

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_16)

(149-206)

Tetratricopeptide repeat (TPR_19)

(89-147)

Tetratricopeptide repeat (TPR_8)

(39-68)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

68.284

100

0.688
  pilF Acinetobacter baylyi ADP1

64.286

100

0.643

Multiple sequence alignment