Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   BY40_RS08350 Genome accession   NZ_CP051001
Coordinates   1712438..1712878 (-) Length   146 a.a.
NCBI ID   WP_000360904.1    Uniprot ID   P36647
Organism   Escherichia coli O157:H16 str. 98-3133     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1707438..1717878
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BY40_RS08325 coaE 1707928..1708548 (-) 621 WP_001269520.1 dephospho-CoA kinase -
  BY40_RS08330 - 1708573..1708617 (+) 45 WP_120795372.1 protein YacM -
  BY40_RS08335 guaC 1708773..1709816 (+) 1044 WP_001217338.1 GMP reductase -
  BY40_RS08340 hofC 1709851..1711053 (-) 1203 WP_000157266.1 protein transport protein HofC -
  BY40_RS08345 pilB 1711043..1712428 (-) 1386 WP_001025145.1 type II secretion system protein GspE Machinery gene
  BY40_RS08350 pilA 1712438..1712878 (-) 441 WP_000360904.1 prepilin peptidase-dependent pilin Machinery gene
  BY40_RS08355 nadC 1713081..1713974 (-) 894 WP_001135174.1 carboxylating nicotinate-nucleotide diphosphorylase -
  BY40_RS08360 ampD 1714062..1714613 (+) 552 WP_000923721.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  BY40_RS08365 ampE 1714610..1715464 (+) 855 WP_000172005.1 beta-lactamase regulator AmpE -
  BY40_RS08370 aroP 1715507..1716880 (-) 1374 WP_000969915.1 aromatic amino acid transporter AroP -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15621.75 Da        Isoelectric Point: 4.3938

>NTDB_id=382504 BY40_RS08350 WP_000360904.1 1712438..1712878(-) (pilA) [Escherichia coli O157:H16 str. 98-3133]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTR
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=382504 BY40_RS08350 WP_000360904.1 1712438..1712878(-) (pilA) [Escherichia coli O157:H16 str. 98-3133]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAGATACCTGCGACGGTGGCAGCAATGGCATTCCCTCGCCTACCACCACCCGC
TATGTTTCAGCCATGAGTGTGGCAAAGGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGGCTAAGCGTCGTCAT
GACACCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGCAACTGCAATATTCAAAGTGACAGCGCATTGCAGC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P36647

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

43.089

84.247

0.363