Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   PPE_RS14540 Genome accession   NC_014483
Coordinates   3268226..3268663 (-) Length   145 a.a.
NCBI ID   WP_013310962.1    Uniprot ID   -
Organism   Paenibacillus polymyxa E681     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 3263226..3273663
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PPE_RS14525 (PPE_02993) - 3264467..3266092 (-) 1626 WP_013310959.1 response regulator -
  PPE_RS14530 (PPE_02994) - 3266067..3267887 (-) 1821 WP_043882246.1 sensor histidine kinase -
  PPE_RS14535 (PPE_02995) - 3268004..3268204 (+) 201 WP_013310961.1 hypothetical protein -
  PPE_RS14540 (PPE_02996) nucA/comI 3268226..3268663 (-) 438 WP_013310962.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  PPE_RS14545 (PPE_02997) - 3268804..3268998 (+) 195 WP_013310963.1 hypothetical protein -
  PPE_RS14550 (PPE_02998) - 3269386..3269688 (+) 303 WP_013310964.1 hypothetical protein -
  PPE_RS14555 (PPE_02999) - 3269758..3271644 (-) 1887 WP_013310965.1 hypothetical protein -
  PPE_RS14560 (PPE_03000) - 3271765..3272187 (-) 423 WP_013310966.1 hypothetical protein -
  PPE_RS14565 (PPE_03001) - 3272204..3272872 (-) 669 WP_013310967.1 uracil-DNA glycosylase -

Sequence

Protein


Download         Length: 145 a.a.        Molecular weight: 15898.13 Da        Isoelectric Point: 9.4243

>NTDB_id=38249 PPE_RS14540 WP_013310962.1 3268226..3268663(-) (nucA/comI) [Paenibacillus polymyxa E681]
MKKKLLSFIALVLLVLGAYWFEGGNLLSKTTGVNPPSSSQVTLQFPSGRYPETAEHIKEAIQAGKSSICTIDREGAEQNR
KHSLAGVPTRKGYDRDEWPMAMCAEGGKGANVKYIAPKDNRGAGSWVSHQLDEFEDGTRVKFVVK

Nucleotide


Download         Length: 438 bp        

>NTDB_id=38249 PPE_RS14540 WP_013310962.1 3268226..3268663(-) (nucA/comI) [Paenibacillus polymyxa E681]
TTGAAGAAAAAATTGCTAAGTTTTATAGCTTTAGTGCTGCTAGTTTTGGGCGCGTACTGGTTTGAAGGAGGCAATCTTCT
TTCGAAAACGACAGGAGTAAACCCGCCTTCATCGTCTCAGGTTACGCTGCAATTTCCGTCAGGTCGTTATCCTGAAACGG
CGGAGCATATCAAGGAAGCCATTCAGGCGGGGAAATCATCCATATGCACAATTGACCGCGAAGGAGCCGAGCAAAATCGC
AAGCATTCGCTTGCAGGAGTTCCCACTCGTAAAGGATACGATCGGGACGAATGGCCGATGGCTATGTGCGCAGAAGGTGG
CAAAGGTGCAAATGTCAAATACATAGCTCCTAAGGATAATCGTGGGGCAGGGTCATGGGTCAGTCACCAATTGGATGAGT
TTGAGGATGGAACTCGCGTAAAATTTGTCGTCAAATAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(48-141)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

57.377

84.138

0.483

Multiple sequence alignment