Detailed information    

insolico Bioinformatically predicted

Overview

Name   recO   Type   Machinery gene
Locus tag   BSUW23_RS12700 Genome accession   NC_014479
Coordinates   2464928..2465695 (-) Length   255 a.a.
NCBI ID   WP_003226211.1    Uniprot ID   E0U4P1
Organism   Bacillus spizizenii str. W23     
Function   ssDNA annealing; plasmid transformation (predicted from homology)   
DNA processing Homologous recombination

Genomic Context

Location: 2459928..2470695
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BSUW23_RS12680 (BSUW23_12505) - 2460102..2460914 (-) 813 WP_003226219.1 pyruvate, water dikinase regulatory protein -
  BSUW23_RS12685 (BSUW23_12510) ccpN 2460940..2461578 (-) 639 WP_003226217.1 transcriptional regulator CcpN -
  BSUW23_RS12690 (BSUW23_12515) glyS 2461712..2463751 (-) 2040 WP_003226215.1 glycine--tRNA ligase subunit beta -
  BSUW23_RS12695 (BSUW23_12520) glyQ 2463744..2464631 (-) 888 WP_003226213.1 glycine--tRNA ligase subunit alpha -
  BSUW23_RS12700 (BSUW23_12525) recO 2464928..2465695 (-) 768 WP_003226211.1 DNA repair protein RecO Machinery gene
  BSUW23_RS12705 (BSUW23_12530) - 2465732..2465875 (-) 144 WP_003230053.1 YqzL family protein -
  BSUW23_RS12710 (BSUW23_12535) era 2466020..2466925 (-) 906 WP_003226207.1 GTPase Era -
  BSUW23_RS12715 (BSUW23_12540) - 2466906..2467316 (-) 411 WP_003226204.1 cytidine deaminase -
  BSUW23_RS12720 (BSUW23_12545) - 2467437..2467808 (-) 372 WP_003226203.1 diacylglycerol kinase family protein -
  BSUW23_RS12725 (BSUW23_12550) ybeY 2467789..2468262 (-) 474 WP_003226199.1 rRNA maturation RNase YbeY -
  BSUW23_RS12730 (BSUW23_12555) pgpH 2468263..2470398 (-) 2136 WP_079996420.1 cyclic-di-AMP phosphodiesterase PgpH -

Sequence

Protein


Download         Length: 255 a.a.        Molecular weight: 29305.97 Da        Isoelectric Point: 8.3072

>NTDB_id=38202 BSUW23_RS12700 WP_003226211.1 2464928..2465695(-) (recO) [Bacillus spizizenii str. W23]
MLTKCEGIVLRTNDYGETNKIVTLLTREHGKIGVMARGAKKPNSRLSAVSQPFLYGSFLMQKTSGLGTLQQGEMILSMRG
IREDLFLTAYAAYIAELVDKGTEEKKPNPYLFEFILESFKQLDEGTDPDVITFIAQMKMLGVMGLYPELNHCVHCKSQDG
TFHFSVRDNGFICHRCFEKDPYRVPIKPQTARLLRLFYYFDLSRLGNVSLKQETKAELKQVIDLYYEEYSGLYLKSKRFL
DQMESMKHLMGENKS

Nucleotide


Download         Length: 768 bp        

>NTDB_id=38202 BSUW23_RS12700 WP_003226211.1 2464928..2465695(-) (recO) [Bacillus spizizenii str. W23]
ATGCTGACAAAATGTGAAGGAATCGTTCTTCGCACAAATGATTACGGAGAGACGAATAAAATCGTTACATTACTGACAAG
AGAACACGGAAAAATAGGTGTTATGGCAAGAGGCGCCAAAAAACCGAACAGCCGTTTATCAGCAGTCAGCCAGCCCTTTT
TGTATGGTTCATTTTTAATGCAAAAAACATCAGGCCTTGGAACGCTTCAGCAGGGTGAAATGATCCTCAGTATGAGAGGC
ATCAGGGAAGATTTGTTTTTAACAGCATATGCTGCGTATATTGCTGAGCTAGTCGACAAAGGCACAGAAGAAAAGAAGCC
AAACCCTTATTTGTTTGAATTCATTTTAGAATCATTTAAACAGCTGGATGAAGGAACAGATCCGGATGTCATCACCTTCA
TTGCTCAAATGAAGATGCTCGGTGTCATGGGGCTGTATCCTGAGCTGAATCACTGTGTGCATTGTAAAAGCCAGGACGGA
ACCTTTCATTTTTCTGTTCGTGATAACGGTTTTATTTGCCATCGCTGCTTTGAAAAAGACCCTTACAGGGTTCCAATTAA
ACCGCAAACGGCAAGGCTTTTGAGACTGTTTTATTACTTCGATCTATCGAGGCTGGGCAATGTGTCCTTAAAGCAGGAAA
CAAAAGCTGAATTAAAGCAAGTAATTGATTTGTATTATGAAGAATATTCAGGGTTATATTTAAAATCAAAGCGCTTTTTA
GATCAAATGGAAAGCATGAAACATCTAATGGGTGAAAACAAAAGTTGA

Domains

Predicted by InterproScan.

Recombination protein O C terminal (RecO_C)

(82-237)

Recombination protein O N terminal (RecO_N)

(1-75)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB E0U4P1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recO Bacillus subtilis subsp. subtilis str. 168

97.255

100

0.973

Multiple sequence alignment