Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   FRD49_RS15950 Genome accession   NZ_CP042994
Coordinates   3320133..3320933 (-) Length   266 a.a.
NCBI ID   WP_004710659.1    Uniprot ID   -
Organism   Acinetobacter nosocomialis strain J1A     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3315133..3325933
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FRD49_RS15930 (FRD49_15930) - 3316198..3316896 (-) 699 WP_002053293.1 YfgM family protein -
  FRD49_RS15935 (FRD49_15935) hisS 3316925..3318217 (-) 1293 WP_106479672.1 histidine--tRNA ligase -
  FRD49_RS15940 (FRD49_15940) ispG 3318214..3319329 (-) 1116 WP_032009832.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  FRD49_RS15945 (FRD49_15945) - 3319351..3320142 (-) 792 WP_002053491.1 helix-turn-helix domain-containing protein -
  FRD49_RS15950 (FRD49_15950) pilF 3320133..3320933 (-) 801 WP_004710659.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  FRD49_RS15955 (FRD49_15955) rlmN 3320949..3322181 (-) 1233 WP_002053401.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  FRD49_RS15960 (FRD49_15960) ndk 3322304..3322735 (-) 432 WP_000963851.1 nucleoside-diphosphate kinase -
  FRD49_RS15965 (FRD49_15965) iscX 3322849..3323049 (-) 201 WP_000524329.1 Fe-S cluster assembly protein IscX -
  FRD49_RS15970 (FRD49_15970) - 3323149..3323700 (-) 552 WP_004710660.1 phosphatase PAP2 family protein -
  FRD49_RS15975 (FRD49_15975) - 3323725..3325023 (-) 1299 WP_079744809.1 glycosyltransferase family 4 protein -

Sequence

Protein


Download         Length: 266 a.a.        Molecular weight: 30097.44 Da        Isoelectric Point: 9.2307

>NTDB_id=380049 FRD49_RS15950 WP_004710659.1 3320133..3320933(-) (pilF) [Acinetobacter nosocomialis strain J1A]
MSTPKLKIMLCMGVAVAFLASGCQSSPTLKKDPEKAVKVRTQLAAEYIRSGDLDSAKRSLDQALSVDSRDATANMMMGIL
LQQEGSKPNLEKAEHYFKRAISSEPDNAQARNNYGTYLYQMERYNDAIEQFRIAGATLGYDQRYQALENLGRIYLKLGDI
ASAEKTFKQALLANRDSYISMLELAEIFYLQQQIPAATQMYEQYVRTVGQKNQGARALWIGLRVARANADKMGMQVLVNQ
LRALFPESPEYQRYLQLQYSTEAVWK

Nucleotide


Download         Length: 801 bp        

>NTDB_id=380049 FRD49_RS15950 WP_004710659.1 3320133..3320933(-) (pilF) [Acinetobacter nosocomialis strain J1A]
TTGAGTACTCCGAAGTTAAAGATCATGTTATGTATGGGGGTTGCTGTTGCATTTCTGGCCAGTGGTTGTCAGTCTTCGCC
AACGCTTAAAAAGGACCCGGAAAAAGCAGTTAAGGTGCGTACGCAGCTTGCCGCAGAATACATTCGTTCTGGTGACTTGG
ACTCTGCAAAGCGTTCCCTTGACCAAGCTTTAAGTGTCGATAGTCGTGATGCAACGGCAAATATGATGATGGGCATTTTA
TTGCAACAAGAAGGTAGTAAACCCAATTTAGAAAAAGCAGAACATTATTTTAAACGTGCAATTTCATCTGAGCCAGATAA
TGCTCAGGCGCGTAATAATTATGGTACGTATTTGTACCAAATGGAGCGTTATAATGATGCCATTGAGCAATTCCGTATTG
CTGGTGCAACATTAGGTTATGATCAACGCTATCAAGCCTTAGAAAATCTAGGGCGGATTTATTTAAAATTAGGTGATATT
GCGAGTGCTGAAAAAACATTCAAACAAGCACTCCTTGCTAACCGCGATTCTTACATTTCAATGTTAGAGTTAGCAGAAAT
TTTTTATTTGCAGCAGCAGATTCCGGCTGCTACACAAATGTATGAACAATACGTTCGTACAGTGGGGCAGAAAAATCAGG
GTGCTCGCGCACTTTGGATTGGTTTAAGAGTGGCTCGAGCCAATGCGGATAAAATGGGAATGCAAGTTTTAGTAAACCAG
TTACGTGCTTTATTCCCGGAAAGTCCAGAATATCAACGTTATTTGCAATTACAGTATAGTACTGAGGCCGTATGGAAATA
A

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_8)

(39-68)

Tetratricopeptide repeat (TPR_16)

(89-134)

Tetratricopeptide repeat (TPR_16)

(149-206)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

98.496

100

0.985
  pilF Acinetobacter baylyi ADP1

74.615

97.744

0.729

Multiple sequence alignment