Detailed information NTG0037998

Bioinformatically predicted

Overview

• Name: pilA
• Type: Machinery gene
• Locus tag: TOCE_RS05295
• Genome accession: NC_014377
• Coordinates: 1044294..1045190 (+)
• Length: 298 a.a.
• NCBI ID: WP_013275865.1
• Uniprot ID: D9S348
• Organism: Thermosediminibacter oceani DSM 16646
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1039294..1050190

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
TOCE_RS05280 (Toce_1060)	rnc	1039544..1040269 (+)	726	WP_013275862.1	ribonuclease III	-
TOCE_RS05285 (Toce_1061)	-	1040346..1040606 (+)	261	WP_013275863.1	stage V sporulation protein S	-
TOCE_RS05290 (Toce_1062)	smc	1040714..1044271 (+)	3558	WP_013275864.1	chromosome segregation protein SMC	-
TOCE_RS05295 (Toce_1063)	pilA	1044294..1045190 (+)	897	WP_013275865.1	signal recognition particle-docking protein FtsY	Machinery gene
TOCE_RS05300 (Toce_1064)	ylxM	1045264..1045623 (+)	360	WP_013275866.1	YlxM family DNA-binding protein	-
TOCE_RS05305 (Toce_1065)	ffh	1045625..1046965 (+)	1341	WP_013275867.1	signal recognition particle protein	-
TOCE_RS05310 (Toce_1066)	rpsP	1046980..1047279 (+)	300	WP_013275868.1	30S ribosomal protein S16	-
TOCE_RS05315 (Toce_1067)	-	1047283..1047510 (+)	228	WP_013275869.1	KH domain-containing protein	-
TOCE_RS05320 (Toce_1068)	rimM	1047564..1048076 (+)	513	WP_013275870.1	ribosome maturation factor RimM	-
TOCE_RS05325 (Toce_1069)	trmD	1048076..1048819 (+)	744	WP_013275871.1	tRNA (guanosine(37)-N1)-methyltransferase TrmD	-
TOCE_RS05330 (Toce_1070)	rplS	1048869..1049210 (+)	342	WP_013275872.1	50S ribosomal protein L19	-
TOCE_RS05335 (Toce_1071)	ylqF	1049250..1050089 (+)	840	WP_013275873.1	ribosome biogenesis GTPase YlqF	-

Sequence

Protein

• Length: 298 a.a.
• Molecular weight: 32588.82 Da
• Isoelectric Point: 5.9881

>NTDB_id=37998 TOCE_RS05295 WP_013275865.1 1044294..1045190(+) (pilA) [Thermosediminibacter oceani DSM 16646]
MGFFERLKQSLAKTRNSLAEKVESLLKFSTKIDEETLEELEEILITADIGVDATEKLISRLREKVKAEKIKSPEELKEAL
KNEIIKVFPEPKFDIIPPSVILIVGVNGVGKTTTIGKLAFKFKSEGKKVILAAADTFRAAATEQLSIWGQRVGVDVIKHQ
EGSDPASVLFDAIQAGKARRADIIICDTAGRLHTRKNLMEELKKLYRVSEKEYPEARISSLLVLDATTGQNAIAQARIFK
EAVNISGIILTKLDGTAKGGVAVAIASELNLPVLFVGVGEGVDDLQEFDPKAFVDAIF

Nucleotide

• Length: 897 bp

>NTDB_id=37998 TOCE_RS05295 WP_013275865.1 1044294..1045190(+) (pilA) [Thermosediminibacter oceani DSM 16646]
ATGGGTTTTTTTGAGCGCCTAAAGCAGAGCCTTGCAAAAACCAGAAACTCCTTGGCCGAAAAAGTAGAAAGCCTTTTGAA
ATTCTCAACAAAAATTGATGAGGAGACCCTCGAAGAACTTGAAGAAATTCTTATAACCGCTGATATAGGTGTTGATGCCA
CGGAAAAACTCATCTCTCGTCTAAGGGAGAAGGTAAAGGCAGAAAAGATTAAATCTCCGGAAGAACTGAAGGAAGCCCTG
AAAAACGAGATCATAAAAGTCTTTCCTGAGCCGAAGTTCGATATTATTCCCCCGTCGGTTATACTCATAGTGGGAGTAAA
CGGCGTGGGAAAAACCACTACAATAGGCAAACTGGCTTTTAAATTCAAATCTGAAGGCAAAAAAGTGATCCTCGCCGCTG
CTGACACCTTCAGAGCGGCGGCGACCGAACAGCTTTCGATCTGGGGACAGAGAGTGGGTGTGGATGTTATAAAGCATCAG
GAAGGATCAGATCCGGCATCGGTTTTATTCGACGCTATACAGGCAGGTAAAGCCCGCCGCGCGGATATTATAATCTGCGA
TACTGCCGGCAGGTTGCACACCAGGAAAAACCTTATGGAGGAGCTTAAAAAACTCTACAGGGTCAGCGAAAAGGAGTACC
CAGAAGCGAGAATTTCGAGCTTGCTGGTGCTGGATGCCACCACCGGACAGAACGCTATAGCGCAGGCCAGGATTTTTAAA
GAAGCCGTAAATATCTCCGGGATCATCCTCACAAAACTGGACGGTACGGCAAAAGGCGGTGTGGCCGTTGCCATCGCTTC
GGAATTAAATCTGCCGGTGCTTTTCGTCGGGGTCGGAGAGGGTGTGGACGACCTGCAGGAATTCGACCCGAAAGCCTTTG
TGGATGCGATATTTTAA

Domains

Predicted by InterproScan.

• SRP54-type protein, GTPase domain (SRP54) (98-298)

• SRP54-type protein, helical bundle domain (SRP54_N) (18-84)

3D structure

• AlphaFold DB: D9S348

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilA	Neisseria gonorrhoeae MS11	49.175	100	0.5