Detailed information NTG0379476

Bioinformatically predicted

Overview

• Name: rpoS
• Type: Regulator
• Locus tag: HB638_RS07965
• Genome accession: NZ_CP050382
• Coordinates: 1661176..1662168 (+)
• Length: 330 a.a.
• NCBI ID: WP_000081550.1
• Uniprot ID: A0A370V765
• Organism: Escherichia coli strain 52148
• Function: regulation of chitinases (predicted from homology) Competence regulation

Genomic Context

Location: 1656176..1667168

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
HB638_RS07935 (HB638_07930)	ispD	1656237..1656947 (+)	711	WP_000246138.1	2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase	-
HB638_RS07940 (HB638_07935)	ispF	1656947..1657426 (+)	480	WP_001219242.1	2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase	-
HB638_RS07945 (HB638_07940)	truD	1657423..1658472 (+)	1050	WP_000568943.1	tRNA pseudouridine(13) synthase TruD	-
HB638_RS07950 (HB638_07945)	surE	1658453..1659214 (+)	762	WP_001295182.1	5'/3'-nucleotidase SurE	-
HB638_RS07955 (HB638_07950)	pcm	1659208..1659834 (+)	627	WP_000254708.1	protein-L-isoaspartate O-methyltransferase	-
HB638_RS07960 (HB638_07955)	nlpD	1660019..1661113 (+)	1095	WP_306174120.1	murein hydrolase activator NlpD	-
HB638_RS07965 (HB638_07960)	rpoS	1661176..1662168 (+)	993	WP_000081550.1	RNA polymerase sigma factor RpoS	Regulator
HB638_RS07970 (HB638_07965)	ygbN	1662262..1663626 (-)	1365	WP_000104441.1	GntP family transporter	-
HB638_RS07975 (HB638_07970)	ygbM	1663715..1664491 (-)	777	WP_001136934.1	HPr family phosphocarrier protein	-
HB638_RS07980 (HB638_07975)	ygbL	1664496..1665134 (-)	639	WP_001278994.1	aldolase	-
HB638_RS07985 (HB638_07980)	otnK	1665131..1666393 (-)	1263	WP_000590403.1	3-oxo-tetronate kinase	-

Sequence

Protein

• Length: 330 a.a.
• Molecular weight: 37972.84 Da
• Isoelectric Point: 4.5597

>NTDB_id=379476 HB638_RS07965 WP_000081550.1 1661176..1662168(+) (rpoS) [Escherichia coli strain 52148]
MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEELLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFA
RRALRGDVASRRRMIESNLRLVVKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNERITSVDTPLGGDSEKALLDILAD
EKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLARRFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQ
GLNIEALFRE

Nucleotide

• Length: 993 bp

>NTDB_id=379476 HB638_RS07965 WP_000081550.1 1661176..1662168(+) (rpoS) [Escherichia coli strain 52148]
ATGAGTCAGAATACGCTGAAAGTTCATGATTTAAATGAAGATGCGGAATTTGATGAGAACGGAGTTGAGGTTTTTGACGA
AAAGGCCTTAGTAGAAGAGGAACCCAGTGATAACGATTTGGCCGAAGAGGAACTGTTATCGCAGGGAGCCACACAGCGTG
TGTTGGACGCGACTCAGCTTTACCTTGGTGAGATTGGTTATTCACCACTGTTAACGGCCGAAGAAGAAGTTTATTTTGCG
CGTCGCGCACTGCGTGGAGATGTCGCCTCTCGCCGCCGGATGATCGAGAGTAACTTGCGTCTGGTGGTAAAAATTGCCCG
CCGTTATGGCAATCGTGGTCTGGCGTTGCTGGACCTTATCGAAGAGGGCAACCTGGGGCTGATCCGCGCGGTAGAGAAGT
TTGACCCGGAACGTGGTTTCCGCTTCTCAACATACGCAACCTGGTGGATTCGCCAGACGATTGAACGGGCGATTATGAAC
CAAACCCGTACTATTCGTTTGCCGATTCACATCGTAAAGGAGCTGAACGTTTACCTGCGAACCGCACGTGAGTTGTCCCA
TAAGCTGGACCATGAACCAAGTGCGGAAGAGATCGCAGAGCAACTGGATAAGCCAGTTGATGACGTCAGCCGTATGCTTC
GTCTTAACGAGCGCATTACCTCGGTAGACACCCCGCTGGGTGGTGATTCCGAAAAAGCGTTGCTGGACATCCTGGCCGAT
GAAAAAGAGAACGGTCCGGAAGATACCACGCAAGATGACGATATGAAGCAGAGCATCGTCAAATGGCTGTTCGAGCTGAA
CGCCAAACAGCGTGAAGTGCTGGCACGTCGATTCGGTTTGCTGGGGTACGAAGCGGCAACACTGGAAGATGTAGGTCGTG
AAATTGGCCTCACCCGTGAACGTGTTCGCCAGATTCAGGTTGAAGGCCTGCGCCGTTTGCGCGAAATCCTGCAAACGCAG
GGGCTGAATATCGAAGCGCTGTTCCGCGAGTAA

Domains

Predicted by InterProScan.

• Sigma-70 region 3 (Sigma70_r3) (174-248)

• Sigma-70 region 2 (Sigma70_r2) (94-163)

• Sigma-70 factor, region 1.2 (Sigma70_r1_2) (56-88)

• Sigma-70, region 4 (Sigma70_r4) (262-315)

3D structure

• AlphaFold DB: A0A370V765

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
rpoS	Vibrio cholerae O1 biovar El Tor strain E7946	73.065	97.879	0.715