Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   FTX45_RS20110 Genome accession   NZ_CP042930
Coordinates   4249014..4250447 (+) Length   477 a.a.
NCBI ID   WP_032614481.1    Uniprot ID   A0A3E1ZUH7
Organism   Leclercia adecarboxylata strain J656     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4244014..4255447
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FTX45_RS20080 (FTX45_20085) - 4244560..4245537 (+) 978 WP_032614470.1 calcium/sodium antiporter -
  FTX45_RS20085 (FTX45_20090) kdsD 4245551..4246537 (+) 987 WP_032614472.1 arabinose-5-phosphate isomerase KdsD -
  FTX45_RS20090 (FTX45_20095) kdsC 4246552..4247118 (+) 567 WP_077226309.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  FTX45_RS20095 (FTX45_20100) lptC 4247115..4247690 (+) 576 WP_032614475.1 LPS export ABC transporter periplasmic protein LptC -
  FTX45_RS20100 (FTX45_20105) lptA 4247659..4248234 (+) 576 WP_032614476.1 lipopolysaccharide ABC transporter substrate-binding protein LptA -
  FTX45_RS20105 (FTX45_20110) lptB 4248241..4248966 (+) 726 WP_032614478.1 LPS export ABC transporter ATP-binding protein -
  FTX45_RS20110 (FTX45_20115) rpoN/rpoN1 4249014..4250447 (+) 1434 WP_032614481.1 RNA polymerase factor sigma-54 Machinery gene
  FTX45_RS20115 (FTX45_20120) hpf 4250470..4250757 (+) 288 WP_032614483.1 ribosome hibernation promoting factor -
  FTX45_RS20120 (FTX45_20125) ptsN 4250840..4251331 (+) 492 WP_032614485.1 PTS IIA-like nitrogen regulatory protein PtsN -
  FTX45_RS20125 (FTX45_20130) rapZ 4251377..4252231 (+) 855 WP_032614487.1 RNase adapter RapZ -
  FTX45_RS20130 (FTX45_20135) npr 4252228..4252500 (+) 273 WP_032614489.1 PTS phosphocarrier protein NPr -
  FTX45_RS20135 (FTX45_20140) mtgA 4252497..4253222 (-) 726 WP_394854182.1 monofunctional biosynthetic peptidoglycan transglycosylase -
  FTX45_RS20140 (FTX45_20145) elbB 4253219..4253872 (-) 654 WP_150872562.1 isoprenoid biosynthesis glyoxalase ElbB -

Sequence

Protein


Download         Length: 477 a.a.        Molecular weight: 54186.01 Da        Isoelectric Point: 4.3155

>NTDB_id=379474 FTX45_RS20110 WP_032614481.1 4249014..4250447(+) (rpoN/rpoN1) [Leclercia adecarboxylata strain J656]
MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALDSNPLLEQTDLHDEVETQQTQDNETLDSVDALEQKEMPD
ELPLDASWDEIYTAGTPSGTRADYQDDELPVYQGETTQSLQDYLMWQVELTPFSDTDRAIATSIVDAVDETGYLTVPLDD
ILESIGDDEIDLEEVEAVLKRVQRFDPIGVAARDLRDCLLIQLSQFVKETPWLEEARLIVSEHLDLLANHDFRSLMRVTR
LKEEILKEAVNLIQSLDPRPGQSIQTSEPEYVIPDVLVRKHNGLWTVELNSDSIPRLQINQQYASMCTSSRNDSDNQFIR
SNLQEARWLIKSLESRNDTLLRVSRCIVEQQQAFFEQGEEFMKPMVLADIAQAVEMHESTISRVTTQKYLHSPRGIFELK
YFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTTMLSDQGIMVARRTVAKYRESLSIPPSNQRKQLV

Nucleotide


Download         Length: 1434 bp        

>NTDB_id=379474 FTX45_RS20110 WP_032614481.1 4249014..4250447(+) (rpoN/rpoN1) [Leclercia adecarboxylata strain J656]
ATGAAGCAAGGTTTGCAATTAAGGCTCAGCCAACAGCTGGCAATGACGCCGCAGTTACAACAGGCCATTCGTCTGTTGCA
GCTGTCGACGCTAGAACTTCAGCAGGAGCTTCAGCAAGCGCTGGACAGTAATCCCCTGCTTGAGCAAACCGATCTTCACG
ACGAGGTAGAAACCCAGCAAACGCAGGATAACGAAACGCTCGACAGCGTCGACGCGCTGGAGCAAAAAGAGATGCCAGAC
GAGCTTCCTCTTGATGCCAGCTGGGATGAGATCTACACCGCCGGCACGCCATCCGGTACGCGCGCTGATTACCAGGATGA
TGAGTTGCCGGTGTATCAGGGCGAAACCACCCAGTCCCTCCAGGATTACCTGATGTGGCAGGTGGAGCTGACTCCGTTTT
CCGATACCGATCGCGCCATTGCCACCTCCATTGTCGATGCCGTCGATGAGACCGGCTATTTAACCGTGCCGCTGGACGAC
ATTCTGGAAAGCATCGGTGATGATGAGATCGATCTTGAAGAGGTCGAAGCGGTCCTGAAGCGCGTGCAGCGTTTTGATCC
GATCGGCGTTGCGGCACGTGATTTACGTGACTGCCTGCTGATTCAGCTTTCGCAGTTCGTCAAAGAAACGCCGTGGCTGG
AAGAGGCTCGTCTGATCGTCAGCGAGCATCTGGATCTGCTGGCCAACCACGACTTCCGCTCGTTGATGCGGGTGACGCGA
TTGAAAGAAGAGATCCTGAAAGAGGCGGTCAATCTTATTCAGTCGTTGGATCCGCGTCCGGGGCAATCCATCCAGACAAG
CGAGCCTGAGTACGTTATTCCCGATGTACTGGTACGCAAGCATAACGGCCTGTGGACCGTGGAGCTGAATTCCGACAGCA
TCCCGCGCCTGCAAATTAATCAGCAATACGCCTCGATGTGCACCAGTTCGCGCAACGACTCGGATAACCAGTTTATCCGC
AGCAACCTGCAGGAGGCGCGCTGGCTGATCAAAAGCCTCGAGAGCCGCAATGATACGCTGCTACGCGTGAGCCGCTGCAT
CGTAGAGCAACAGCAGGCCTTCTTTGAACAAGGTGAAGAATTTATGAAACCGATGGTGCTGGCCGATATCGCCCAGGCCG
TCGAGATGCATGAATCAACGATTTCCCGCGTGACGACGCAGAAGTATCTGCACAGCCCGCGCGGTATTTTTGAGCTTAAG
TATTTTTTCTCCAGCCATGTCAATACCGAAGGCGGTGGTGAAGCTTCGTCTACAGCCATCCGGGCGCTGGTGAAAAAATT
GATCGCCGCGGAAAACCCCGCGAAACCACTGAGCGACAGTAAGTTAACCACCATGCTGTCCGATCAAGGTATCATGGTGG
CACGCCGTACTGTTGCGAAGTATCGAGAGTCTTTATCCATTCCGCCGTCCAACCAGCGTAAACAACTGGTCTGA

Domains

Predicted by InterproScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(317-475)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-303)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A3E1ZUH7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

45.347

100

0.48

Multiple sequence alignment