Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   HB730_RS12935 Genome accession   NZ_CP050332
Coordinates   2705291..2707018 (+) Length   575 a.a.
NCBI ID   WP_058176465.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain DVT413     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2700291..2712018
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HB730_RS12915 (HB730_12975) - 2701002..2701931 (-) 930 WP_003160458.1 LysR family transcriptional regulator -
  HB730_RS12920 (HB730_12980) - 2701954..2702928 (-) 975 WP_023099501.1 quinone oxidoreductase -
  HB730_RS12925 (HB730_12985) - 2703154..2703906 (+) 753 WP_003110095.1 class I SAM-dependent methyltransferase -
  HB730_RS12930 (HB730_12990) - 2704498..2705301 (+) 804 WP_003097732.1 ABC transporter permease -
  HB730_RS12935 (HB730_12995) pilF 2705291..2707018 (+) 1728 WP_058176465.1 GspE/PulE family protein Machinery gene
  HB730_RS12940 (HB730_13000) - 2707023..2708210 (+) 1188 WP_058176466.1 type II secretion system F family protein -
  HB730_RS12945 (HB730_13005) gspG 2708264..2708698 (+) 435 WP_003110089.1 type II secretion system major pseudopilin GspG -
  HB730_RS12950 (HB730_13010) - 2708667..2709077 (+) 411 WP_003110088.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  HB730_RS12955 (HB730_13015) - 2709077..2709502 (+) 426 WP_003110087.1 type II secretion system protein J -
  HB730_RS12960 (HB730_13020) - 2709499..2710089 (+) 591 WP_033957552.1 type II secretion system protein J -
  HB730_RS12965 (HB730_13025) - 2710079..2711158 (+) 1080 WP_058176467.1 general secretion pathway protein GspK -

Sequence

Protein


Download         Length: 575 a.a.        Molecular weight: 63677.83 Da        Isoelectric Point: 5.3319

>NTDB_id=379067 HB730_RS12935 WP_058176465.1 2705291..2707018(+) (pilF) [Pseudomonas aeruginosa strain DVT413]
MSSEAVSPGPQVTAEWIPLGQRLLERGLVGGQELERALDLQRRLGGRLGGILVRSGAISENTLMQVLAEQLRLPLVGADL
RKPSEESIATFLLSTPINTDWFVEEQLVVWEEGEQLLFAARDPLSPSIRETLGYFYPERSIQAVLCRSQDLDGWLEHSLD
LARQGADRHAYGSEDIRYLRELAEEAPVVELVNNVMSQAMEKRSSDIHIEPREFDFRIRFRVDGVLHDQLSLPRERFAAV
VLRIKLISAIDIAERRLPQDGRMSIRVSGTELDVRVSTLPGVHGESVVMRLLPKEREGLRLDRLGMLPDHLRRMRAWVAE
PHGIILVTGPTGSGKSTTLYGALEEINDGVRKLISVEDPVEYQVPNITQVQVHADIGLTFAAALRSILRQDPDVIMIGEI
RDLETAEIAVQSSLTGHLVLSTLHTNDAVSAFTRLIDMGVEPFLVASPVRGVQAQRLVRRLCAHCAEPCEPTLAAADLAA
TEAATARLFPGQAAQWRVARGCGRCEGTGYSGRVGIYELVDLSSEMRDLIVQRASLEQMHRLAASQGQRNLREDGLIKAW
LGMTSLDEVHRVTSG

Nucleotide


Download         Length: 1728 bp        

>NTDB_id=379067 HB730_RS12935 WP_058176465.1 2705291..2707018(+) (pilF) [Pseudomonas aeruginosa strain DVT413]
ATGTCCTCTGAGGCCGTTTCCCCGGGCCCGCAGGTGACGGCCGAGTGGATACCGCTGGGGCAGCGTTTGCTCGAACGCGG
ATTGGTGGGCGGCCAGGAACTCGAGCGGGCGCTCGACCTGCAACGCCGGCTTGGCGGCCGCCTGGGAGGCATTCTCGTGC
GTTCCGGAGCCATCTCGGAAAATACCCTCATGCAGGTGCTGGCGGAGCAACTGCGGTTGCCGCTGGTGGGAGCCGACCTG
CGGAAGCCGAGCGAAGAGTCCATCGCCACATTCCTGCTCAGCACCCCGATCAATACCGACTGGTTCGTCGAAGAGCAACT
GGTGGTCTGGGAGGAGGGCGAGCAACTGCTGTTCGCGGCGCGTGATCCGTTATCTCCTTCGATACGCGAAACCCTGGGCT
ATTTCTATCCGGAGCGCTCGATTCAAGCAGTGCTGTGCCGCTCCCAGGATCTGGACGGCTGGTTGGAGCATTCCCTGGAC
CTGGCCCGGCAAGGTGCGGATCGGCACGCCTACGGCAGCGAAGACATCCGCTACCTGCGGGAGCTGGCGGAGGAGGCGCC
GGTAGTCGAACTCGTCAACAACGTGATGAGCCAGGCCATGGAAAAGCGCTCTTCGGATATTCACATCGAACCCCGGGAGT
TCGACTTCCGTATCCGCTTCCGCGTCGACGGCGTCCTGCACGACCAACTGAGCCTTCCCCGCGAGCGATTCGCGGCGGTC
GTCTTGCGGATCAAGCTGATCTCCGCCATCGACATCGCGGAGCGACGGTTGCCGCAGGATGGACGCATGAGCATCCGGGT
CAGCGGTACGGAGCTCGACGTGCGGGTCTCCACCTTGCCAGGGGTGCATGGCGAATCGGTGGTCATGCGCCTGTTGCCGA
AGGAGCGCGAGGGGTTGCGCCTGGACCGGCTCGGGATGCTCCCGGACCACTTGCGGAGGATGCGTGCATGGGTAGCGGAA
CCACACGGCATCATCCTGGTGACCGGCCCCACCGGATCGGGCAAGTCCACGACGCTCTATGGCGCGCTGGAGGAAATCAA
TGATGGCGTGCGCAAGCTCATCAGCGTGGAAGATCCCGTGGAGTACCAGGTGCCCAACATCACCCAGGTCCAGGTCCATG
CCGACATCGGCCTGACCTTCGCTGCCGCCTTGCGCTCGATCCTGCGGCAGGATCCCGATGTGATCATGATCGGGGAGATA
CGCGACCTGGAGACGGCGGAGATCGCCGTACAGTCCTCGCTCACCGGTCACCTGGTGCTTTCGACCCTGCATACCAACGA
TGCGGTGAGTGCCTTCACGCGCTTGATCGACATGGGCGTGGAACCCTTCCTGGTGGCTTCGCCGGTGCGCGGTGTCCAGG
CCCAGCGACTGGTGCGCAGGTTGTGCGCCCACTGCGCGGAACCTTGCGAGCCAACCCTTGCTGCGGCCGATCTGGCAGCG
ACCGAGGCGGCCACCGCGCGGCTGTTCCCGGGGCAGGCAGCGCAATGGCGCGTGGCGCGAGGCTGCGGCCGCTGCGAAGG
TACCGGCTACAGCGGCCGTGTGGGCATCTACGAACTCGTCGATCTGTCTTCCGAGATGCGCGACCTGATCGTGCAGAGAG
CTTCCCTCGAACAGATGCATCGGCTTGCCGCCAGCCAGGGACAGCGCAATCTGCGTGAAGATGGCTTGATCAAGGCCTGG
TTGGGTATGACCAGTCTTGACGAGGTGCATCGCGTGACCAGCGGCTAA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(194-460)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(52-160)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Thermus thermophilus HB27

41.519

98.435

0.409
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.448

98.609

0.379

Multiple sequence alignment