Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   FR761_RS03830 Genome accession   NZ_CP042556
Coordinates   822097..822897 (+) Length   266 a.a.
NCBI ID   WP_005135836.1    Uniprot ID   -
Organism   Acinetobacter baumannii strain E47     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 817097..827897
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FR761_RS03805 (FR761_03805) - 818007..819305 (+) 1299 WP_086231294.1 glycosyltransferase family 4 protein -
  FR761_RS03810 (FR761_03810) - 819330..819881 (+) 552 WP_000669564.1 phosphatase PAP2 family protein -
  FR761_RS03815 (FR761_03815) iscX 819981..820181 (+) 201 WP_000524330.1 Fe-S cluster assembly protein IscX -
  FR761_RS03820 (FR761_03820) ndk 820295..820726 (+) 432 WP_000963851.1 nucleoside-diphosphate kinase -
  FR761_RS03825 (FR761_03825) rlmN 820849..822081 (+) 1233 WP_000093084.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  FR761_RS03830 (FR761_03830) pilF 822097..822897 (+) 801 WP_005135836.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  FR761_RS03835 (FR761_03835) - 822888..823679 (+) 792 WP_000405163.1 helix-turn-helix domain-containing protein -
  FR761_RS03840 (FR761_03840) ispG 823701..824816 (+) 1116 WP_000572095.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  FR761_RS03845 (FR761_03845) hisS 824813..826105 (+) 1293 WP_000095251.1 histidine--tRNA ligase -
  FR761_RS03850 (FR761_03850) - 826134..826832 (+) 699 WP_000058788.1 YfgM family protein -

Sequence

Protein


Download         Length: 266 a.a.        Molecular weight: 30120.52 Da        Isoelectric Point: 9.2307

>NTDB_id=378411 FR761_RS03830 WP_005135836.1 822097..822897(+) (pilF) [Acinetobacter baumannii strain E47]
MSTPKLKIMLCMGVAVALLASGCQTLQTVKKDPEKAVKVRTQLAAEYIRSGDLDSAKRSLDQALSVDSRDATANMMMGIL
LQQEGSKPNLEKAEHYFKRAISSEPDNAQARNNYGTYLYQMERYNDAIEQFRIAGATLGYDQRYQALENLGRIYLKLGDI
ASAEKTFKQALLANRDSYISMLELAEIFYLQQQIPAATQMYEQYVRTVGQKNQGARALWIGLRVARANADKMGMQVLVNQ
LRALFPESPEYQRYLQLQYSTEAVWK

Nucleotide


Download         Length: 801 bp        

>NTDB_id=378411 FR761_RS03830 WP_005135836.1 822097..822897(+) (pilF) [Acinetobacter baumannii strain E47]
TTGAGTACTCCGAAGTTGAAGATCATGTTATGTATGGGGGTTGCTGTTGCACTTTTGGCAAGTGGTTGTCAGACTTTGCA
AACGGTTAAAAAGGACCCGGAAAAAGCAGTAAAAGTGCGTACGCAGCTTGCTGCCGAATACATTCGTTCTGGTGACTTGG
ATTCTGCAAAGCGTTCCCTTGACCAAGCGTTAAGTGTCGATAGTCGTGATGCAACGGCAAATATGATGATGGGTATTTTA
TTGCAACAAGAAGGCAGTAAACCCAATTTAGAAAAAGCGGAACATTACTTTAAGCGTGCAATTTCGTCTGAACCAGACAA
TGCTCAGGCACGTAATAATTATGGTACGTATTTGTACCAAATGGAGCGTTATAATGATGCTATTGAGCAATTTCGTATTG
CTGGTGCAACATTAGGCTATGATCAACGTTACCAGGCCTTAGAAAATCTGGGGCGAATTTATTTAAAACTAGGCGATATT
GCGAGTGCTGAAAAAACATTCAAACAAGCACTTCTTGCTAACCGCGACTCTTACATTTCAATGTTAGAGTTAGCAGAAAT
TTTTTATTTGCAGCAGCAGATTCCGGCTGCTACACAAATGTATGAACAATATGTTCGTACAGTGGGGCAGAAAAATCAGG
GTGCACGCGCACTTTGGATTGGTTTAAGAGTGGCTCGAGCCAATGCGGATAAAATGGGAATGCAAGTTTTAGTAAACCAG
TTACGTGCTTTATTCCCGGAAAGTCCAGAATATCAACGTTATTTGCAATTACAGTATAGTACTGAGGCCGTATGGAAATA
A

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_8)

(39-68)

Tetratricopeptide repeat (TPR_16)

(89-134)

Tetratricopeptide repeat (TPR_16)

(149-206)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

99.624

100

0.996
  pilF Acinetobacter baylyi ADP1

74.231

97.744

0.726

Multiple sequence alignment