Detailed information NTG0378253

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: FR744_RS20980
• Genome accession: NZ_CP042520
• Coordinates: 4237257..4238057 (+)
• Length: 266 a.a.
• NCBI ID: WP_004145898.1
• Uniprot ID: -
• Organism: Klebsiella pneumoniae strain C2
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4232257..4243057

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
FR744_RS20945 (FR744_20945)	gspH	4232413..4232928 (+)	516	WP_004191951.1	type II secretion system minor pseudopilin GspH	-
FR744_RS20950 (FR744_20950)	gspI	4232925..4233290 (+)	366	WP_002889025.1	type II secretion system minor pseudopilin GspI	-
FR744_RS20955 (FR744_20955)	gspJ	4233287..4233883 (+)	597	WP_009309266.1	type II secretion system minor pseudopilin GspJ	-
FR744_RS20960 (FR744_20960)	gspK	4233880..4234866 (+)	987	WP_077258397.1	type II secretion system minor pseudopilin GspK	-
FR744_RS20965 (FR744_20965)	gspL	4234853..4236046 (+)	1194	WP_040224338.1	type II secretion system protein GspL	-
FR744_RS20970 (FR744_20970)	-	4236039..4236524 (+)	486	WP_004151943.1	type II secretion system protein M	-
FR744_RS20975 (FR744_20975)	-	4236514..4237260 (+)	747	WP_004145897.1	type II secretion system protein N	-
FR744_RS20980 (FR744_20980)	pilD	4237257..4238057 (+)	801	WP_004145898.1	prepilin peptidase	Machinery gene
FR744_RS20985 (FR744_20985)	hrpB	4238105..4240534 (-)	2430	WP_062920977.1	ATP-dependent helicase HrpB	-
FR744_RS20990 (FR744_20990)	thpR	4240607..4241143 (+)	537	WP_004145901.1	RNA 2',3'-cyclic phosphodiesterase	-
FR744_RS20995 (FR744_20995)	sfsA	4241143..4241859 (+)	717	WP_002888848.1	DNA/RNA nuclease SfsA	-
FR744_RS21000 (FR744_21000)	dksA	4242022..4242477 (+)	456	WP_002888845.1	RNA polymerase-binding protein DksA	-

Sequence

Protein

• Length: 266 a.a.
• Molecular weight: 29389.01 Da
• Isoelectric Point: 8.8894

>NTDB_id=378253 FR744_RS20980 WP_004145898.1 4237257..4238057(+) (pilD) [Klebsiella pneumoniae strain C2]
MTTLAALSLHFPFVWYGFLLLFGLALGSFYNVVIYRLPRMLTQTADDERITLSTPGSSCPQCRQPIAWRDNIPLLSFLWL
GRRARCCQAPIAWSYPLTELATGLLFILAGALLAPGLPLAGGLVLLSFLLILARIDARTQLLPDRLTLPLLWAGLLFNLN
EVYIALPDAVAGAMAGYLALWSVYWLFRLLTGKEALGYGDFKLLAALGAWCGWQVLPQVLLLASASGLVWTLLQRLWTRQ
SLQQPLAFGPWLALAGGGIFLWQQMV

Nucleotide

• Length: 801 bp

>NTDB_id=378253 FR744_RS20980 WP_004145898.1 4237257..4238057(+) (pilD) [Klebsiella pneumoniae strain C2]
ATGACGACACTAGCCGCGTTATCGCTGCACTTTCCCTTTGTCTGGTATGGCTTTCTGCTGCTGTTCGGCCTGGCGCTGGG
CAGCTTTTACAATGTGGTGATCTATCGTCTGCCGCGCATGTTGACCCAGACCGCGGACGACGAGCGGATAACCCTCAGCA
CCCCGGGATCTTCCTGCCCGCAGTGCCGCCAGCCGATCGCCTGGCGGGACAATATCCCGCTGCTCAGTTTCCTCTGGCTG
GGCCGTCGCGCCCGCTGCTGTCAGGCGCCCATCGCGTGGAGCTATCCGCTGACCGAGCTGGCCACCGGCCTGCTGTTTAT
CCTCGCCGGAGCTTTGCTGGCGCCGGGCCTGCCGCTGGCGGGCGGCCTGGTGCTGCTCTCGTTCCTGCTGATACTGGCGC
GGATTGACGCCCGGACCCAGCTGCTGCCCGATCGCCTGACTCTGCCCCTGCTGTGGGCCGGGCTGCTGTTCAATCTTAAC
GAGGTGTACATTGCCCTGCCGGATGCGGTCGCCGGGGCGATGGCCGGTTATCTGGCGCTGTGGTCGGTCTACTGGCTGTT
CCGCCTGCTGACCGGGAAAGAGGCGCTGGGCTATGGCGATTTTAAGCTGCTGGCGGCGCTGGGCGCCTGGTGCGGCTGGC
AGGTTCTGCCGCAGGTGCTGTTGCTCGCTTCGGCCAGCGGGCTGGTGTGGACCCTGCTGCAACGCCTGTGGACCCGGCAG
TCGCTGCAGCAGCCGCTGGCCTTCGGCCCCTGGCTGGCGCTGGCCGGCGGGGGTATTTTTCTCTGGCAGCAGATGGTCTA
G

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (21-109)

• Type IV leader peptidase family (Peptidase_A24) (123-231)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	49.434	99.624	0.492
pilD	Vibrio campbellii strain DS40M4	46.182	100	0.477
pilD	Acinetobacter nosocomialis M2	46.008	98.872	0.455
pilD	Acinetobacter baumannii D1279779	45.247	98.872	0.447
pilD	Neisseria gonorrhoeae MS11	43.295	98.12	0.425