Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   FR819_RS21330 Genome accession   NZ_CP042493
Coordinates   4433258..4434691 (+) Length   477 a.a.
NCBI ID   WP_032614481.1    Uniprot ID   A0A3E1ZUH7
Organism   Leclercia adecarboxylata strain E61     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4428258..4439691
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FR819_RS21300 (FR819_21300) - 4428804..4429781 (+) 978 WP_032614470.1 calcium/sodium antiporter -
  FR819_RS21305 (FR819_21305) kdsD 4429795..4430781 (+) 987 WP_032614472.1 arabinose-5-phosphate isomerase KdsD -
  FR819_RS21310 (FR819_21310) kdsC 4430796..4431362 (+) 567 WP_077226309.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  FR819_RS21315 (FR819_21315) lptC 4431359..4431934 (+) 576 WP_032614475.1 LPS export ABC transporter periplasmic protein LptC -
  FR819_RS21320 (FR819_21320) lptA 4431903..4432478 (+) 576 WP_032614476.1 lipopolysaccharide ABC transporter substrate-binding protein LptA -
  FR819_RS21325 (FR819_21325) lptB 4432485..4433210 (+) 726 WP_032614478.1 LPS export ABC transporter ATP-binding protein -
  FR819_RS21330 (FR819_21330) rpoN/rpoN1 4433258..4434691 (+) 1434 WP_032614481.1 RNA polymerase factor sigma-54 Machinery gene
  FR819_RS21335 (FR819_21335) hpf 4434714..4435001 (+) 288 WP_032614483.1 ribosome hibernation promoting factor -
  FR819_RS21340 (FR819_21340) ptsN 4435084..4435575 (+) 492 WP_032614485.1 PTS IIA-like nitrogen regulatory protein PtsN -
  FR819_RS21345 (FR819_21345) rapZ 4435621..4436475 (+) 855 WP_032614487.1 RNase adapter RapZ -
  FR819_RS21350 (FR819_21350) npr 4436472..4436744 (+) 273 WP_032614489.1 PTS phosphocarrier protein NPr -
  FR819_RS21355 (FR819_21355) mtgA 4436741..4437466 (-) 726 WP_151586560.1 monofunctional biosynthetic peptidoglycan transglycosylase -
  FR819_RS21360 (FR819_21360) elbB 4437463..4438116 (-) 654 WP_151586562.1 isoprenoid biosynthesis glyoxalase ElbB -

Sequence

Protein


Download         Length: 477 a.a.        Molecular weight: 54186.01 Da        Isoelectric Point: 4.3155

>NTDB_id=378161 FR819_RS21330 WP_032614481.1 4433258..4434691(+) (rpoN/rpoN1) [Leclercia adecarboxylata strain E61]
MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALDSNPLLEQTDLHDEVETQQTQDNETLDSVDALEQKEMPD
ELPLDASWDEIYTAGTPSGTRADYQDDELPVYQGETTQSLQDYLMWQVELTPFSDTDRAIATSIVDAVDETGYLTVPLDD
ILESIGDDEIDLEEVEAVLKRVQRFDPIGVAARDLRDCLLIQLSQFVKETPWLEEARLIVSEHLDLLANHDFRSLMRVTR
LKEEILKEAVNLIQSLDPRPGQSIQTSEPEYVIPDVLVRKHNGLWTVELNSDSIPRLQINQQYASMCTSSRNDSDNQFIR
SNLQEARWLIKSLESRNDTLLRVSRCIVEQQQAFFEQGEEFMKPMVLADIAQAVEMHESTISRVTTQKYLHSPRGIFELK
YFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTTMLSDQGIMVARRTVAKYRESLSIPPSNQRKQLV

Nucleotide


Download         Length: 1434 bp        

>NTDB_id=378161 FR819_RS21330 WP_032614481.1 4433258..4434691(+) (rpoN/rpoN1) [Leclercia adecarboxylata strain E61]
ATGAAGCAAGGTTTGCAATTAAGGCTCAGCCAACAGCTGGCAATGACGCCGCAGTTACAACAGGCCATTCGTCTGTTGCA
GCTGTCGACGCTAGAACTTCAGCAGGAGCTTCAGCAAGCGCTGGACAGTAATCCCCTGCTTGAGCAAACCGATCTTCACG
ACGAGGTAGAAACCCAGCAAACGCAGGATAACGAAACGCTCGACAGCGTCGACGCGCTGGAGCAAAAAGAGATGCCTGAC
GAGCTTCCTCTTGATGCCAGCTGGGATGAGATCTACACCGCCGGTACGCCATCCGGTACGCGCGCCGATTACCAGGATGA
TGAGTTACCGGTGTATCAGGGCGAAACCACCCAGTCCCTCCAGGATTACCTGATGTGGCAGGTGGAGCTAACACCGTTTT
CCGATACCGATCGCGCCATTGCCACCTCCATTGTCGATGCCGTCGATGAGACCGGCTATTTAACCGTGCCGCTGGACGAC
ATTCTGGAAAGCATCGGTGATGATGAGATCGATCTTGAAGAGGTCGAAGCGGTCCTGAAGCGCGTGCAGCGTTTTGATCC
GATTGGCGTTGCGGCACGTGATTTACGTGACTGCCTGCTGATTCAGCTTTCGCAGTTCGTCAAAGAGACGCCGTGGCTGG
AAGAGGCTCGTCTGATCGTCAGCGAGCATCTGGATCTGCTGGCCAACCACGACTTCCGCTCGTTGATGCGCGTGACGCGA
TTGAAAGAAGAGATCCTGAAAGAGGCGGTCAATCTTATTCAGTCGTTGGATCCGCGTCCGGGGCAATCCATCCAGACAAG
CGAGCCTGAGTACGTTATTCCCGATGTACTGGTACGCAAGCATAACGGCCTGTGGACCGTGGAGCTGAATTCCGACAGCA
TCCCGCGCCTGCAAATTAATCAGCAATATGCCTCGATGTGCACCAGTTCGCGCAACGACTCGGATAACCAGTTTATCCGC
AGCAACCTGCAGGAGGCGCGCTGGCTGATCAAAAGCCTCGAGAGCCGCAATGATACGCTGCTACGCGTGAGCCGCTGCAT
CGTAGAGCAACAGCAGGCCTTCTTTGAACAAGGTGAAGAATTTATGAAACCGATGGTGCTGGCGGATATCGCCCAGGCCG
TCGAGATGCATGAATCAACGATTTCCCGCGTGACGACGCAGAAGTATCTGCACAGCCCGCGCGGTATTTTTGAGCTTAAG
TATTTTTTCTCCAGCCATGTCAATACCGAAGGCGGTGGCGAAGCTTCTTCTACAGCCATCCGGGCGCTGGTGAAAAAATT
GATCGCCGCGGAAAACCCCGCGAAACCACTGAGCGACAGTAAGTTAACCACCATGCTGTCCGATCAAGGTATCATGGTGG
CACGCCGTACTGTTGCGAAGTATCGAGAGTCTTTATCCATTCCGCCGTCCAACCAGCGTAAACAACTGGTCTGA

Domains

Predicted by InterproScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(317-475)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-303)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A3E1ZUH7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

45.347

100

0.48

Multiple sequence alignment