Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   FGL80_RS03440 Genome accession   NZ_CP042420
Coordinates   677938..679305 (+) Length   455 a.a.
NCBI ID   WP_077281988.1    Uniprot ID   A0A5B8TEG9
Organism   Leuconostoc lactis strain CBA3622     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 672938..684305
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FGL80_RS03410 (FGL80_03410) - 673261..674685 (-) 1425 WP_055308460.1 aspartate ammonia-lyase -
  FGL80_RS03415 (FGL80_03415) rpiA 674848..675537 (-) 690 WP_029510095.1 ribose-5-phosphate isomerase RpiA -
  FGL80_RS03420 (FGL80_03420) - 675534..675815 (-) 282 WP_010002509.1 GNAT family N-acetyltransferase -
  FGL80_RS03425 (FGL80_03425) - 676041..676580 (+) 540 WP_055308459.1 deoxyuridine 5'-triphosphate nucleotidohydrolase -
  FGL80_RS03430 (FGL80_03430) - 676588..676950 (+) 363 WP_244297954.1 NUDIX domain-containing protein -
  FGL80_RS03435 (FGL80_03435) - 677070..677951 (+) 882 WP_055308458.1 metallophosphoesterase family protein -
  FGL80_RS03440 (FGL80_03440) radA 677938..679305 (+) 1368 WP_077281988.1 DNA repair protein RadA Machinery gene
  FGL80_RS03445 (FGL80_03445) - 679316..679639 (+) 324 WP_055308457.1 hypothetical protein -
  FGL80_RS03450 (FGL80_03450) gltX 679815..681311 (+) 1497 WP_055308549.1 glutamate--tRNA ligase -
  FGL80_RS03455 (FGL80_03455) - 681447..682352 (+) 906 WP_055308456.1 NADP-dependent oxidoreductase -
  FGL80_RS03460 (FGL80_03460) adhP 682515..683570 (+) 1056 WP_055308455.1 alcohol dehydrogenase AdhP -
  FGL80_RS03465 (FGL80_03465) - 683731..684270 (+) 540 WP_055308454.1 hypothetical protein -

Sequence

Protein


Download         Length: 455 a.a.        Molecular weight: 49227.40 Da        Isoelectric Point: 5.2659

>NTDB_id=377815 FGL80_RS03440 WP_077281988.1 677938..679305(+) (radA) [Leuconostoc lactis strain CBA3622]
MAKVKTQFICSNCGFTSARYLGRCSNCGEWGTFVEEKIMPETNDRKSRVSLDGRTAKVEKINEVTSEETPRVATNLKELN
RVLGGGVVPGSMVLIGGDPGIGKSTLLLQVSGQLAHEGRVLYVTGEESATQVKLRADRLGVGNDEFYLYPETDMTAIKKQ
IESLQPDFVVIDSVQTMQEPDVTSAIGSVSQIREVTADLLQIAKTNNISIFIVGHVTKDGAIAGPKILEHMVDTVLYFEG
DSNYKYRILRAVKNRFGATNELGIFEMRDGGLIEVANPSEIFLEERLAGATGSAIVVALEGSRPILVELQALVTPTVFGN
AQRTASGLDRNRVSLIMAVLEKRANLLLQNQDAYLKAAGGVKLDEPAIDLAIAVALASSYHDKESRPSDVFVGEIGLTGE
IRSVADIESRLKEARKLGFKRAIVPKNNLNGVTLPEGIQVIGVATLREALQLALD

Nucleotide


Download         Length: 1368 bp        

>NTDB_id=377815 FGL80_RS03440 WP_077281988.1 677938..679305(+) (radA) [Leuconostoc lactis strain CBA3622]
ATCGCAAAAGTTAAAACACAATTTATTTGCTCAAACTGTGGTTTCACGTCAGCGCGCTATCTGGGTCGCTGCTCAAACTG
TGGTGAATGGGGCACATTTGTCGAAGAAAAAATCATGCCAGAGACCAATGATCGGAAAAGTCGGGTGAGCTTAGATGGTC
GTACGGCCAAAGTTGAAAAAATTAATGAAGTCACATCAGAGGAAACACCGCGTGTTGCCACGAACCTTAAAGAACTTAAC
CGCGTGTTAGGTGGTGGGGTTGTCCCTGGTTCAATGGTGCTCATTGGTGGGGATCCAGGCATTGGGAAATCAACCCTCTT
GCTCCAAGTCTCTGGGCAATTGGCCCATGAAGGTCGTGTGTTATACGTGACTGGGGAAGAATCCGCTACCCAAGTAAAGT
TGCGGGCAGATCGCTTGGGTGTGGGGAATGATGAATTTTACCTCTATCCAGAAACAGATATGACGGCGATTAAAAAGCAA
ATTGAATCGCTGCAACCAGATTTTGTCGTGATTGACTCAGTACAAACGATGCAGGAACCAGACGTGACGTCTGCCATTGG
CTCCGTGTCACAAATCCGCGAAGTCACGGCAGATTTATTGCAAATTGCTAAAACCAATAATATTTCTATTTTTATCGTTG
GCCACGTGACCAAAGATGGGGCGATTGCTGGACCAAAGATTTTGGAGCATATGGTCGATACGGTGCTCTATTTTGAAGGC
GATAGCAACTACAAGTACCGCATTTTACGGGCGGTGAAAAATCGTTTTGGCGCCACCAATGAATTGGGTATTTTCGAAAT
GCGCGACGGCGGTTTGATTGAGGTCGCCAACCCATCAGAAATCTTTTTGGAAGAACGATTAGCTGGCGCAACTGGTTCGG
CCATTGTGGTCGCCTTGGAAGGCTCACGGCCGATCTTGGTTGAATTACAGGCCTTGGTGACGCCTACCGTCTTTGGAAAC
GCGCAACGAACGGCATCTGGATTAGATCGTAATCGCGTGTCGTTAATTATGGCCGTTTTGGAAAAGCGCGCTAATCTCTT
GCTGCAAAATCAAGACGCTTATCTGAAAGCTGCTGGTGGGGTCAAGCTGGATGAACCAGCAATTGATTTGGCAATTGCCG
TGGCGTTGGCCAGTTCTTATCATGATAAAGAATCGCGGCCAAGCGACGTCTTTGTTGGTGAAATCGGGCTAACTGGGGAA
ATCCGGAGCGTCGCTGATATTGAAAGTCGGTTGAAGGAAGCGCGCAAGCTCGGCTTTAAACGGGCGATTGTACCAAAGAA
CAACTTGAATGGGGTCACGCTCCCTGAAGGAATTCAAGTGATTGGCGTCGCCACGTTAAGGGAAGCGTTGCAATTAGCCT
TAGATTAA

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(70-220)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

Subunit ChlI of Mg-chelatase (ChlI)

(362-428)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A5B8TEG9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus pneumoniae Rx1

66.962

99.121

0.664
  radA Streptococcus pneumoniae D39

66.962

99.121

0.664
  radA Streptococcus pneumoniae R6

66.962

99.121

0.664
  radA Streptococcus pneumoniae TIGR4

66.962

99.121

0.664
  radA Streptococcus mitis NCTC 12261

66.741

99.121

0.662
  radA Streptococcus mitis SK321

66.741

99.121

0.662
  radA Bacillus subtilis subsp. subtilis str. 168

62.281

100

0.624

Multiple sequence alignment