Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   FGL73_RS07845 Genome accession   NZ_CP042408
Coordinates   1648212..1649573 (-) Length   453 a.a.
NCBI ID   WP_003130563.1    Uniprot ID   -
Organism   Lactococcus lactis strain CBA3619     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1643212..1654573
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FGL73_RS07810 (FGL73_07810) - 1643759..1644253 (+) 495 WP_038602308.1 GNAT family N-acetyltransferase -
  FGL73_RS07815 (FGL73_07815) - 1644345..1644668 (-) 324 WP_146978274.1 DUF1048 domain-containing protein -
  FGL73_RS07820 (FGL73_07820) - 1644655..1645002 (-) 348 WP_098394202.1 DUF1048 domain-containing protein -
  FGL73_RS07825 (FGL73_07825) - 1644989..1645351 (-) 363 WP_003129889.1 PadR family transcriptional regulator -
  FGL73_RS07830 (FGL73_07830) - 1645472..1646101 (-) 630 WP_021723075.1 hypothetical protein -
  FGL73_RS07835 (FGL73_07835) - 1646166..1647251 (-) 1086 WP_038602323.1 PIN/TRAM domain-containing protein -
  FGL73_RS07840 (FGL73_07840) - 1647482..1648168 (+) 687 WP_146978275.1 YoaK family protein -
  FGL73_RS07845 (FGL73_07845) radA 1648212..1649573 (-) 1362 WP_003130563.1 DNA repair protein RadA Machinery gene
  FGL73_RS07855 (FGL73_07855) rplQ 1650200..1650580 (-) 381 WP_003130558.1 50S ribosomal protein L17 -
  FGL73_RS07860 (FGL73_07860) - 1650599..1651537 (-) 939 WP_003130557.1 DNA-directed RNA polymerase subunit alpha -
  FGL73_RS07865 (FGL73_07865) rpsK 1651585..1651968 (-) 384 WP_010906297.1 30S ribosomal protein S11 -
  FGL73_RS07870 (FGL73_07870) rpsM 1651989..1652354 (-) 366 WP_003130555.1 30S ribosomal protein S13 -
  FGL73_RS07875 (FGL73_07875) rpmJ 1652372..1652488 (-) 117 WP_001808836.1 50S ribosomal protein L36 -
  FGL73_RS07880 (FGL73_07880) infA 1652542..1652760 (-) 219 WP_003130554.1 translation initiation factor IF-1 -
  FGL73_RS07885 (FGL73_07885) - 1653059..1653706 (-) 648 WP_010906298.1 adenylate kinase -

Sequence

Protein


Download         Length: 453 a.a.        Molecular weight: 49893.28 Da        Isoelectric Point: 5.1748

>NTDB_id=377714 FGL73_RS07845 WP_003130563.1 1648212..1649573(-) (radA) [Lactococcus lactis strain CBA3619]
MAKKKSSFVCQNCGYKSAKYLGRCPNCGEWSSFVEEVEVQEVKNQRVSMTGERSKPLKLDEVELFDTPRIETDLDEFNRV
LGGGVVPGSLVLIGGDPGIGKSTLLLQVSTQLASRGRVLYVSGEESAQQIKLRAERLGDIDRDFYLYAETNMQSIRAEVE
RLKPNFLIIDSIQTIMTPEIQSTQGSVSQVREVTGELMQIAKTNDIATFIVGHVTKEGQLAGPRMLEHMVDTVLYFEGER
NNTFRILRAVKNRFGSTNEIGIFEMQGHGLVEVTNPSEVFLEERLEGSTGSAIVCALEGTRPILVEIQALTTPTMFGNAK
RTTSGLDFNRVSLIMAVLEKRTGLLMQNQDAYLKSAGGVKLDEPAIDLAVAVAVASSYKELPTDARECFIGEIGLTGEIR
RVTRIEQRLNEAAKLGFKKVYAPKNSIVGIDIPEQIQVIGVTTLTECLKLVFG

Nucleotide


Download         Length: 1362 bp        

>NTDB_id=377714 FGL73_RS07845 WP_003130563.1 1648212..1649573(-) (radA) [Lactococcus lactis strain CBA3619]
ATGGCGAAGAAAAAATCATCATTTGTTTGTCAAAATTGTGGCTATAAGTCTGCAAAATACTTAGGACGTTGTCCAAACTG
TGGCGAATGGTCTTCTTTTGTTGAAGAAGTTGAAGTCCAAGAAGTTAAAAATCAACGTGTTTCAATGACTGGCGAACGTT
CAAAACCATTGAAGCTAGATGAAGTTGAATTATTTGATACTCCTCGGATTGAAACGGACTTAGATGAATTTAATCGTGTA
TTAGGAGGGGGTGTTGTTCCTGGTTCATTAGTTCTGATTGGGGGAGACCCTGGGATAGGTAAATCAACCTTACTTTTACA
GGTTTCTACTCAATTGGCTTCTAGAGGTCGTGTGCTTTATGTGAGTGGTGAAGAATCAGCCCAACAAATTAAACTAAGAG
CTGAGCGTTTGGGAGATATTGACCGAGACTTCTATCTCTATGCTGAAACAAATATGCAGTCGATTCGTGCAGAAGTTGAG
CGTTTAAAACCTAACTTCTTAATTATTGATTCCATTCAAACAATTATGACACCAGAAATTCAATCAACTCAAGGCTCAGT
AAGTCAAGTTCGTGAAGTGACTGGAGAATTGATGCAAATTGCTAAAACGAATGATATTGCGACCTTTATTGTTGGTCATG
TGACTAAAGAAGGTCAGCTGGCTGGGCCAAGGATGTTAGAGCATATGGTTGATACGGTGCTGTATTTTGAAGGAGAACGA
AATAATACATTTAGAATTCTTCGTGCTGTAAAAAACCGTTTTGGTTCAACTAATGAAATTGGAATTTTTGAGATGCAGGG
TCATGGCCTGGTCGAAGTGACTAATCCCTCAGAGGTCTTTTTAGAAGAACGTTTAGAGGGCTCAACTGGATCTGCAATTG
TTTGTGCACTTGAAGGAACACGGCCCATATTAGTTGAAATACAAGCACTGACGACACCGACAATGTTTGGAAATGCTAAA
AGGACGACCTCAGGCCTAGATTTTAACCGTGTAAGTTTAATTATGGCGGTGCTTGAGAAGAGAACGGGGCTTTTGATGCA
AAATCAAGATGCCTACCTCAAATCTGCGGGGGGAGTAAAATTGGATGAGCCAGCCATTGACCTCGCGGTCGCGGTCGCTG
TGGCTTCAAGTTATAAAGAACTTCCAACAGATGCGCGTGAATGTTTTATTGGTGAAATTGGTTTAACGGGTGAAATTCGT
CGGGTGACCCGAATTGAACAAAGATTGAATGAAGCTGCAAAACTAGGGTTTAAAAAAGTATATGCTCCAAAAAATTCAAT
TGTAGGGATTGATATTCCTGAACAGATTCAAGTGATTGGAGTAACAACTTTAACTGAATGTTTAAAACTAGTCTTTGGCT
AA

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(70-218)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

Subunit ChlI of Mg-chelatase (ChlI)

(360-425)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus pneumoniae Rx1

81.236

100

0.812
  radA Streptococcus pneumoniae D39

81.236

100

0.812
  radA Streptococcus pneumoniae R6

81.236

100

0.812
  radA Streptococcus pneumoniae TIGR4

81.236

100

0.812
  radA Streptococcus mitis SK321

81.236

100

0.812
  radA Streptococcus mitis NCTC 12261

81.015

100

0.81
  radA Bacillus subtilis subsp. subtilis str. 168

61.488

100

0.62

Multiple sequence alignment