Detailed information NTG0037750

Bioinformatically predicted

Overview

• Name: pilA
• Type: Machinery gene
• Locus tag: RPSI07_RS22245
• Genome accession: NC_014311
• Coordinates: 3156162..3157289 (+)
• Length: 375 a.a.
• NCBI ID: WP_013213631.1
• Uniprot ID: A0A1U9VHA8
• Organism: Ralstonia solanacearum PSI07
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3151162..3162289

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
RPSI07_RS22220 (RPSI07_3016)	-	3151537..3151785 (-)	249	WP_013213627.1	YfhL family 4Fe-4S dicluster ferredoxin	-
RPSI07_RS22225 (RPSI07_3017)	coaD	3151836..3152342 (-)	507	WP_003262550.1	pantetheine-phosphate adenylyltransferase	-
RPSI07_RS22230 (RPSI07_3018)	rsmD	3152538..3153185 (-)	648	WP_013213628.1	16S rRNA (guanine(966)-N(2))-methyltransferase RsmD	-
RPSI07_RS22235 (RPSI07_3019)	-	3153265..3154608 (-)	1344	WP_013213629.1	M16 family metallopeptidase	-
RPSI07_RS22240 (RPSI07_3020)	-	3154608..3156101 (-)	1494	WP_013213630.1	M16 family metallopeptidase	-
RPSI07_RS22245 (RPSI07_3021)	pilA	3156162..3157289 (+)	1128	WP_013213631.1	signal recognition particle-docking protein FtsY	Machinery gene
RPSI07_RS22250 (RPSI07_3022)	-	3157286..3157924 (-)	639	WP_013213632.1	LysE/ArgO family amino acid transporter	-
RPSI07_RS22255 (RPSI07_3023)	maiA	3157921..3158571 (-)	651	WP_013213633.1	maleylacetoacetate isomerase	-
RPSI07_RS22260 (RPSI07_3024)	-	3158575..3159276 (-)	702	WP_013213634.1	fumarylacetoacetate hydrolase family protein	-
RPSI07_RS22265 (RPSI07_3025)	-	3159427..3160053 (+)	627	WP_013213635.1	ANTAR domain-containing response regulator	-
RPSI07_RS22270 (RPSI07_3027)	-	3160330..3161592 (+)	1263	WP_013213637.1	CmpA/NrtA family ABC transporter substrate-binding protein	-

Sequence

Protein

• Length: 375 a.a.
• Molecular weight: 39819.04 Da
• Isoelectric Point: 6.1635

>NTDB_id=37750 RPSI07_RS22245 WP_013213631.1 3156162..3157289(+) (pilA) [Ralstonia solanacearum PSI07]
MFSFWKKRKAEPQPAAEPVPPAAAPEAVQAPAPAPVASPAPAPAPVAVPDTPATPAALDMQADDIETVPTPPVVEQARKG
WMSRLRSGLSKTSKGLTTLFVGVKVDEALFEELETALLMADAGVDATEYLLGELRRRVKAQRIETAEGVKTALRDLLIEL
LHPLEKTMVLGREQPTVIMIAGVNGAGKTTSIGKLCKHFQTYGQSVLLAAGDTFRAAAREQLVIWGQRNNVTVVAQESGD
PAAVIFDAVNAARARGIDIVMADTAGRLPTQLHLMEELKKVRRVIGKAMATAPHETLLVIDANTGQNALAQVKAFDDTLG
LTGLIITKLDGTAKGGILAAIARQRPVPVYFIGVGEQVEDLQPFSAREFADALLG

Nucleotide

• Length: 1128 bp

>NTDB_id=37750 RPSI07_RS22245 WP_013213631.1 3156162..3157289(+) (pilA) [Ralstonia solanacearum PSI07]
ATGTTTAGTTTCTGGAAGAAGCGCAAGGCAGAGCCCCAGCCGGCTGCCGAACCGGTGCCGCCCGCCGCCGCACCTGAAGC
GGTACAGGCTCCGGCCCCGGCCCCGGTTGCCTCCCCTGCACCTGCACCCGCCCCGGTTGCCGTGCCGGATACACCGGCCA
CGCCGGCTGCGCTGGACATGCAGGCCGACGATATCGAGACCGTGCCGACGCCGCCCGTGGTGGAGCAGGCGCGCAAGGGC
TGGATGTCGCGCCTGCGCTCGGGCCTGTCGAAGACCAGCAAGGGCCTGACCACGCTGTTCGTCGGCGTGAAGGTGGACGA
GGCGCTGTTCGAGGAGCTGGAAACCGCGCTGCTGATGGCCGATGCAGGCGTCGATGCCACCGAATACCTGCTGGGCGAAC
TGCGCCGCCGTGTGAAAGCCCAGCGCATCGAGACCGCCGAGGGCGTGAAGACCGCGCTGCGCGACCTGCTCATCGAGCTG
CTGCATCCGCTGGAAAAGACCATGGTGCTCGGCCGCGAGCAGCCGACGGTGATCATGATCGCGGGCGTCAACGGCGCCGG
CAAGACCACCAGCATCGGCAAGCTGTGCAAGCACTTCCAGACCTACGGGCAATCCGTGCTGCTGGCAGCGGGCGACACGT
TCCGTGCTGCCGCGCGCGAGCAGTTGGTGATCTGGGGCCAGCGCAACAACGTGACCGTGGTGGCGCAGGAATCGGGCGAC
CCGGCCGCCGTGATCTTCGACGCGGTGAACGCGGCGCGCGCCCGCGGCATCGATATCGTCATGGCCGACACCGCCGGCCG
CCTGCCGACCCAGCTGCACCTGATGGAAGAACTGAAGAAGGTGCGCCGCGTGATCGGCAAGGCGATGGCGACGGCGCCGC
ACGAGACGTTGCTGGTGATCGACGCCAACACCGGCCAGAATGCGCTGGCGCAGGTGAAAGCCTTCGACGATACGCTCGGC
CTGACCGGCCTGATCATCACCAAGCTGGACGGCACCGCCAAGGGCGGCATCCTGGCCGCCATCGCGCGGCAGCGGCCTGT
ACCGGTGTATTTCATCGGCGTGGGCGAACAGGTGGAAGACCTGCAGCCGTTCTCCGCGCGCGAATTCGCCGACGCGCTAC
TGGGCTAG

Domains

Predicted by InterproScan.

• SRP54-type protein, GTPase domain (SRP54) (175-375)

• SRP54-type protein, helical bundle domain (SRP54_N) (94-155)

3D structure

• AlphaFold DB: A0A1U9VHA8

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilA	Neisseria gonorrhoeae MS11	62.951	81.333	0.512