Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   RPSI07_RS22130 Genome accession   NC_014311
Coordinates   3134201..3135700 (+) Length   499 a.a.
NCBI ID   WP_013213612.1    Uniprot ID   A0AAD0S8Y4
Organism   Ralstonia solanacearum PSI07     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3129201..3140700
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RPSI07_RS22105 (RPSI07_2993) - 3130379..3131362 (+) 984 WP_013213607.1 KpsF/GutQ family sugar-phosphate isomerase -
  RPSI07_RS22110 (RPSI07_2994) - 3131395..3131982 (+) 588 WP_013213608.1 KdsC family phosphatase -
  RPSI07_RS22115 (RPSI07_2995) lptC 3131984..3132616 (+) 633 WP_013213609.1 LPS export ABC transporter periplasmic protein LptC -
  RPSI07_RS22120 (RPSI07_2996) lptA 3132685..3133281 (+) 597 WP_013213610.1 lipopolysaccharide transport periplasmic protein LptA -
  RPSI07_RS22125 (RPSI07_2997) lptB 3133315..3134103 (+) 789 WP_013213611.1 LPS export ABC transporter ATP-binding protein -
  RPSI07_RS22130 (RPSI07_2998) rpoN/rpoN1 3134201..3135700 (+) 1500 WP_013213612.1 RNA polymerase factor sigma-54 Machinery gene
  RPSI07_RS22135 (RPSI07_2999) hpf 3135752..3136105 (+) 354 WP_013213613.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  RPSI07_RS22140 (RPSI07_3000) ptsN 3136453..3136908 (+) 456 WP_003269869.1 PTS IIA-like nitrogen regulatory protein PtsN -
  RPSI07_RS22145 (RPSI07_3001) hprK 3136979..3137953 (+) 975 WP_011000367.1 HPr(Ser) kinase/phosphatase -
  RPSI07_RS22150 (RPSI07_3002) - 3138145..3138474 (+) 330 WP_013213614.1 PsiF family protein -
  RPSI07_RS22155 (RPSI07_3003) rapZ 3138567..3139457 (+) 891 WP_013213615.1 RNase adapter RapZ -
  RPSI07_RS22160 (RPSI07_3004) - 3139537..3140187 (+) 651 WP_013213616.1 LON peptidase substrate-binding domain-containing protein -

Sequence

Protein


Download         Length: 499 a.a.        Molecular weight: 55065.44 Da        Isoelectric Point: 4.8496

>NTDB_id=37749 RPSI07_RS22130 WP_013213612.1 3134201..3135700(+) (rpoN/rpoN1) [Ralstonia solanacearum PSI07]
MKQSLQLRLSQHLALTPQLQQSIRLLQLSTIELQQEVEQALTENPLLERENEWLDTSARIGVDGSVSALQSSSATPPPTE
SPPPAANGADHADTAEASYENGSDFDSDYGADRSDWSLDDFARKPQSDEDDRAPMQLREAEPTLREFLMEQLAPLKLSLR
DKGLVIFLIESLDDEGYLTGSLDEILADLPAELEAELDELQAALRMLQSFDPAGIGARSAAECLTLQLQRLDSPAKPLAL
AIVNQHLELLAARDYTRLKKALSVDEGALKAAHELIRSLAPFPGHAFGRAEADFVVPDVVVRKTAAGWMAQLNPDVMPRL
RINDMYAQILRSSRGESGSANLQQKLQEARWLIKNIQQRFDTILRVSQAIVERQKSFFTHGEIAMRPLVLREIADTLGLH
ESTISRVTTNKYMATPLGTFELKYFFGSHVSTETGGAASSTAIRALIKQLVGAEDPKNPLSDSRIAELLGEQGFVVARRT
VAKYREALKIPAVNLRKSL

Nucleotide


Download         Length: 1500 bp        

>NTDB_id=37749 RPSI07_RS22130 WP_013213612.1 3134201..3135700(+) (rpoN/rpoN1) [Ralstonia solanacearum PSI07]
ATGAAACAGTCGCTCCAGCTCCGTCTCTCTCAGCATCTGGCGCTCACGCCGCAACTGCAGCAGTCGATCCGGCTGCTGCA
GCTGTCGACCATCGAACTGCAACAGGAAGTCGAGCAGGCGCTGACTGAGAACCCGCTGCTTGAGCGCGAGAACGAGTGGC
TCGACACCTCAGCGCGCATCGGCGTCGACGGCTCGGTCAGTGCCCTGCAGAGCAGCAGCGCCACCCCGCCGCCGACCGAA
TCGCCGCCGCCCGCCGCCAACGGCGCGGACCACGCCGACACTGCCGAGGCATCGTACGAAAACGGCAGCGATTTCGACAG
CGACTATGGCGCCGATCGTTCCGACTGGAGCCTCGACGACTTCGCACGCAAACCGCAGTCCGACGAAGACGACCGCGCCC
CGATGCAGCTGCGCGAGGCCGAACCCACGCTGCGCGAATTCCTGATGGAACAGCTGGCGCCGCTCAAGCTGTCCCTGCGC
GACAAAGGCCTCGTCATCTTCCTGATCGAATCGCTGGACGACGAAGGCTACCTGACCGGCTCGCTCGATGAGATCCTCGC
CGACCTGCCGGCCGAACTCGAGGCCGAACTCGACGAACTGCAAGCCGCGCTGCGCATGCTGCAGAGCTTCGATCCGGCCG
GCATCGGCGCGCGCTCGGCGGCGGAATGCCTGACGTTGCAGCTGCAGCGCCTGGACTCGCCGGCCAAGCCGCTGGCGCTG
GCCATCGTCAACCAGCATCTCGAACTGCTCGCCGCGCGCGACTACACGCGTCTGAAGAAAGCGCTGTCCGTCGATGAAGG
CGCGCTGAAGGCCGCGCATGAACTGATCCGCTCGCTGGCCCCGTTCCCTGGCCACGCCTTCGGGCGTGCCGAGGCCGACT
TTGTCGTGCCCGACGTGGTGGTGCGTAAAACTGCCGCAGGCTGGATGGCGCAGTTGAACCCCGACGTGATGCCGCGCCTG
CGCATCAACGACATGTACGCGCAGATCCTGCGCAGCAGCCGAGGCGAATCGGGCTCCGCCAACCTGCAGCAGAAGCTGCA
GGAAGCACGCTGGCTGATCAAGAACATCCAACAGCGCTTCGACACCATCCTGCGTGTCTCGCAAGCGATTGTCGAACGTC
AAAAGAGCTTTTTCACGCACGGCGAAATCGCCATGCGCCCCTTGGTTTTGCGGGAAATAGCCGATACACTGGGTCTACAC
GAGTCCACGATCTCCCGTGTGACGACCAACAAGTACATGGCGACCCCCCTGGGGACCTTCGAGCTGAAGTACTTCTTCGG
CAGCCATGTATCGACGGAAACCGGCGGGGCCGCGTCATCCACTGCGATACGCGCGCTCATCAAGCAACTTGTAGGAGCCG
AAGACCCGAAGAATCCTCTGTCCGATAGCAGAATTGCCGAGCTGTTAGGCGAACAAGGATTCGTGGTGGCCCGTCGGACG
GTGGCCAAGTACCGCGAAGCCCTCAAAATCCCTGCAGTGAATTTGCGCAAGTCTTTATAG

Domains

Predicted by InterproScan.

Sigma-54, DNA binding domain (Sigma54_DBD)

(341-497)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(136-326)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

97.395

100

0.974

Multiple sequence alignment