Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   FS593_RS18855 Genome accession   NZ_CP042361
Coordinates   3939485..3940918 (+) Length   477 a.a.
NCBI ID   WP_015960619.1    Uniprot ID   A0A9J9L0T3
Organism   Lelliottia amnigena strain A167     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3934485..3945918
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FS593_RS18825 (FS593_18960) - 3935053..3936030 (+) 978 WP_015960613.1 calcium/sodium antiporter -
  FS593_RS18830 (FS593_18965) kdsD 3936043..3937029 (+) 987 WP_015960614.1 arabinose-5-phosphate isomerase KdsD -
  FS593_RS18835 (FS593_18970) kdsC 3937044..3937610 (+) 567 WP_235135782.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  FS593_RS18840 (FS593_18975) lptC 3937607..3938182 (+) 576 WP_133631563.1 LPS export ABC transporter periplasmic protein LptC -
  FS593_RS18845 (FS593_18980) lptA 3938151..3938705 (+) 555 WP_235134141.1 lipopolysaccharide ABC transporter substrate-binding protein LptA -
  FS593_RS18850 (FS593_18985) lptB 3938712..3939437 (+) 726 WP_015960618.1 LPS export ABC transporter ATP-binding protein -
  FS593_RS18855 (FS593_18990) rpoN/rpoN1 3939485..3940918 (+) 1434 WP_015960619.1 RNA polymerase factor sigma-54 Machinery gene
  FS593_RS18860 (FS593_18995) hpf 3940941..3941228 (+) 288 WP_015960620.1 ribosome hibernation promoting factor -
  FS593_RS18865 (FS593_19000) ptsN 3941311..3941802 (+) 492 WP_015960621.1 PTS IIA-like nitrogen regulatory protein PtsN -
  FS593_RS18870 (FS593_19005) rapZ 3941848..3942702 (+) 855 WP_015960622.1 RNase adapter RapZ -
  FS593_RS18875 (FS593_19010) npr 3942699..3942971 (+) 273 WP_015960623.1 PTS phosphocarrier protein NPr -
  FS593_RS18880 (FS593_19015) mtgA 3943025..3943750 (-) 726 WP_235134142.1 monofunctional biosynthetic peptidoglycan transglycosylase -
  FS593_RS18885 (FS593_19020) elbB 3943747..3944400 (-) 654 WP_235134143.1 isoprenoid biosynthesis glyoxalase ElbB -

Sequence

Protein


Download         Length: 477 a.a.        Molecular weight: 54064.93 Da        Isoelectric Point: 4.3667

>NTDB_id=377390 FS593_RS18855 WP_015960619.1 3939485..3940918(+) (rpoN/rpoN1) [Lelliottia amnigena strain A167]
MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALDSNPLLEQTDLHEEVDTQEKQDTETLDTADALEQKEMPD
ELPLDASWDEIYTAGTPSGTRADYQDDELPVYQGETTQSLQDYLMWQVELTPFTETDRAIATSIVDAVDDTGYLTVTLDE
ILESMGDSEIELDEIEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFAKETPRLDEARLIISEHLDLLANHDFRSLMRVTR
LKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDVLVRKHNGLWTVELNSDSIPRLQINQQYASMCTSARNDSDNQYIR
SNLQEARWLIKSLESRNDTLLRVSRCIVEQQQGFFEQGEEFMKPMVLADIAQAVEMHESTISRVTTQKYLHSPRGIFELK
YFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTTMLSDQGIMVARRTVAKYRESLSIPPSNQRKQLV

Nucleotide


Download         Length: 1434 bp        

>NTDB_id=377390 FS593_RS18855 WP_015960619.1 3939485..3940918(+) (rpoN/rpoN1) [Lelliottia amnigena strain A167]
ATGAAGCAAGGTTTGCAATTAAGGCTCAGCCAACAGTTGGCGATGACGCCACAGTTACAACAGGCGATCCGTCTGTTGCA
ACTGTCAACGTTAGAACTTCAGCAGGAACTGCAGCAAGCGCTGGACAGTAATCCACTGCTGGAGCAAACCGATCTTCATG
AAGAGGTAGATACCCAGGAAAAGCAGGATACCGAAACGCTCGATACGGCAGATGCCCTCGAGCAAAAAGAGATGCCTGAC
GAGTTACCGCTAGATGCCAGCTGGGATGAAATTTACACCGCTGGAACCCCATCCGGAACGCGCGCTGATTATCAGGATGA
TGAGTTACCGGTTTATCAGGGTGAAACCACGCAGTCGCTACAGGATTACCTGATGTGGCAGGTGGAGTTGACCCCTTTCA
CCGAAACCGATCGCGCGATTGCCACTTCCATTGTCGATGCCGTGGATGACACCGGGTATTTAACGGTGACCCTGGACGAA
ATTCTGGAAAGCATGGGTGATAGCGAAATCGAACTCGACGAGATCGAAGCCGTTCTCAAGCGCATTCAACGCTTTGACCC
TGTCGGCGTCGCAGCAAAGGATCTGCGCGACTGTTTGCTGATCCAGCTTTCTCAGTTTGCAAAAGAGACGCCAAGACTCG
ACGAAGCGCGACTGATTATCAGCGAACATTTGGATTTGCTGGCGAATCATGATTTCCGCAGCCTGATGCGCGTGACGCGA
CTCAAAGAAGATGTACTGAAGGAAGCGGTGAATCTGATCCAGTCGCTCGATCCCCGGCCGGGGCAATCGATCCAAACCGG
CGAACCCGAATATGTGATTCCTGACGTTCTGGTCCGAAAGCACAACGGTCTCTGGACCGTAGAACTCAATTCCGACAGCA
TTCCCCGCCTGCAAATCAACCAGCAATATGCTTCCATGTGCACCAGTGCGCGGAACGACTCCGACAATCAATATATTCGT
AGCAATCTTCAGGAAGCGCGATGGCTGATCAAAAGCCTCGAAAGCCGAAATGATACGCTGCTACGCGTGAGTCGCTGCAT
TGTGGAACAGCAGCAAGGCTTTTTTGAGCAGGGCGAAGAATTTATGAAACCGATGGTTTTGGCAGATATTGCTCAAGCCG
TTGAGATGCATGAATCGACCATTTCGCGTGTGACCACGCAGAAGTATCTGCACAGCCCACGTGGTATTTTTGAGCTTAAG
TATTTCTTCTCCAGTCATGTGAATACCGAAGGCGGCGGCGAAGCCTCATCGACGGCAATTCGTGCTCTGGTTAAGAAGTT
GATCGCCGCGGAAAACCCCGCGAAGCCACTGAGTGACAGTAAGTTAACCACCATGCTGTCCGATCAGGGTATCATGGTGG
CACGTCGTACTGTTGCGAAGTATCGAGAGTCTTTATCCATTCCGCCGTCTAACCAGCGTAAACAACTGGTTTGA

Domains

Predicted by InterproScan.

Sigma-54, DNA binding domain (Sigma54_DBD)

(317-475)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-303)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

45.347

100

0.48

Multiple sequence alignment