Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   FOZ74_RS07650 Genome accession   NZ_CP042344
Coordinates   1569968..1571500 (-) Length   510 a.a.
NCBI ID   WP_146912504.1    Uniprot ID   -
Organism   Comamonas flocculans strain NLF-7-7     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1564968..1576500
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FOZ74_RS07620 (FOZ74_07620) - 1565118..1565540 (-) 423 WP_146912498.1 hotdog fold thioesterase -
  FOZ74_RS07625 (FOZ74_07625) - 1565608..1566894 (-) 1287 WP_146912499.1 solute carrier family 23 protein -
  FOZ74_RS07630 (FOZ74_07630) - 1566960..1567451 (-) 492 WP_146912500.1 putative toxin-antitoxin system toxin component, PIN family -
  FOZ74_RS07635 (FOZ74_07635) - 1567474..1568223 (-) 750 WP_146912501.1 M15 family metallopeptidase -
  FOZ74_RS07640 (FOZ74_07640) - 1568220..1569503 (-) 1284 WP_146912502.1 THUMP domain-containing protein -
  FOZ74_RS07645 (FOZ74_07645) - 1569500..1569952 (-) 453 WP_146912503.1 thioesterase family protein -
  FOZ74_RS07650 (FOZ74_07650) rpoN/rpoN1 1569968..1571500 (-) 1533 WP_146912504.1 RNA polymerase factor sigma-54 Machinery gene
  FOZ74_RS07655 (FOZ74_07655) lptB 1571505..1572281 (-) 777 WP_146912505.1 LPS export ABC transporter ATP-binding protein -
  FOZ74_RS07660 (FOZ74_07660) lptA 1572287..1572916 (-) 630 WP_146912506.1 lipopolysaccharide transport periplasmic protein LptA -
  FOZ74_RS07665 (FOZ74_07665) - 1573045..1573692 (-) 648 WP_146912507.1 thiol:disulfide interchange protein DsbA/DsbL -
  FOZ74_RS07670 (FOZ74_07670) - 1573762..1574391 (-) 630 WP_146912508.1 SPOR domain-containing protein -
  FOZ74_RS07675 (FOZ74_07675) argS 1574397..1576076 (-) 1680 WP_146912509.1 arginine--tRNA ligase -

Sequence

Protein


Download         Length: 510 a.a.        Molecular weight: 56437.73 Da        Isoelectric Point: 5.0841

>NTDB_id=377186 FOZ74_RS07650 WP_146912504.1 1569968..1571500(-) (rpoN/rpoN1) [Comamonas flocculans strain NLF-7-7]
MKVGLSLGVSQHLALTPQLQQSIRLLQLSTLELAQEVEAMLVDNPFLERASEEAPAAGAPAPERRTERSEIAPISEAFDA
DPASSGGQNDAETRSDWEGDRELDPSGSDSPWSADAPSRAARREDDEGEASEHESAHETLAEHLHRQALLLRLTRAERAA
LSWLIDSLNDDGYLEDAIESLVETLVGPENTEYLLEMSEHFALALKLLQSLEPAGVGARDLPECLRLQLAALRDTGACPR
AVLEAAMCICEQPLEWLARRDARRLAQACGASEEDTRAAMALIARLEPRPGRRFAQVQHNLVTPDVIVRRRLEGGQVRLD
VQLNPEVVPQLRVHDAYAQALRQHRKGEGHAALQSRLQEARWFVRNIEQRFDTILRTARAIVDKQQAFFLHGELAMRPLV
MREIAEELGLHESTISRVTSAKYMATPQGTYELKYFFGSGLVTDTGGSTSSTAVRALLRQFIAAEDPARPLSDGQLADML
KEQGIACARRTVAKYREQLKIAPASLRKQI

Nucleotide


Download         Length: 1533 bp        

>NTDB_id=377186 FOZ74_RS07650 WP_146912504.1 1569968..1571500(-) (rpoN/rpoN1) [Comamonas flocculans strain NLF-7-7]
ATGAAGGTCGGCCTGTCGCTGGGCGTCTCGCAACACCTTGCACTGACGCCGCAACTGCAGCAGTCCATCCGCCTGCTGCA
ACTGTCCACGCTGGAGCTGGCCCAGGAGGTGGAGGCCATGCTGGTGGACAACCCCTTCCTTGAACGCGCGAGCGAAGAGG
CCCCTGCAGCCGGCGCCCCCGCGCCGGAGCGGCGCACCGAACGCAGCGAGATCGCTCCTATTTCAGAAGCTTTTGACGCA
GACCCTGCAAGCTCTGGAGGCCAAAATGATGCCGAAACCCGGAGCGACTGGGAAGGCGACCGCGAGCTCGACCCCAGCGG
CAGCGACAGCCCCTGGAGCGCCGACGCCCCGAGCCGGGCCGCCCGGCGCGAGGACGACGAGGGCGAGGCGAGCGAGCACG
AGAGCGCGCACGAAACCCTGGCCGAGCACCTGCACCGCCAGGCGCTGCTGCTGCGCCTGACGCGCGCCGAACGCGCGGCG
CTGTCCTGGCTGATCGATTCGCTCAACGACGACGGCTACCTCGAAGACGCCATCGAATCGCTGGTCGAAACCCTGGTCGG
CCCCGAGAACACCGAATACCTGCTGGAGATGAGCGAGCACTTCGCGCTGGCGCTCAAGCTGCTGCAAAGCCTGGAGCCCG
CAGGCGTGGGCGCGCGCGATTTGCCCGAGTGCCTGCGGCTGCAACTGGCGGCGCTGCGCGACACGGGCGCCTGCCCCCGG
GCGGTGCTGGAGGCGGCCATGTGCATCTGCGAGCAGCCGCTCGAATGGCTGGCGCGGCGCGACGCACGGCGGCTGGCGCA
GGCCTGCGGCGCCAGCGAAGAAGACACGCGCGCCGCCATGGCGCTGATCGCCCGGCTCGAACCGCGCCCGGGGCGGCGCT
TTGCCCAGGTGCAGCACAACCTCGTCACGCCCGACGTGATCGTGCGCCGCCGCCTGGAGGGCGGGCAGGTGCGCCTGGAC
GTGCAGCTCAACCCCGAGGTGGTGCCGCAGCTGCGCGTGCACGACGCCTACGCCCAGGCGCTGCGCCAGCACCGCAAGGG
CGAGGGCCACGCCGCGCTGCAAAGCCGCCTGCAGGAGGCGCGCTGGTTCGTCCGCAACATCGAGCAGCGCTTCGACACCA
TCCTGCGCACCGCGCGCGCCATCGTGGACAAGCAGCAGGCCTTCTTCCTGCACGGCGAGCTCGCCATGCGTCCCTTGGTG
ATGCGCGAGATCGCCGAGGAGCTGGGCCTGCACGAATCGACCATCTCGCGCGTGACCAGCGCCAAGTACATGGCCACGCC
CCAGGGCACCTACGAGCTCAAGTACTTCTTCGGCTCGGGTCTGGTGACCGATACCGGCGGCAGCACCTCCAGCACCGCGG
TGCGCGCGCTGCTGCGCCAGTTCATCGCCGCCGAAGACCCGGCGCGGCCGCTGTCCGACGGGCAGCTGGCCGACATGCTC
AAGGAGCAGGGCATCGCCTGCGCGCGGCGCACCGTGGCCAAATACCGCGAACAGCTCAAGATCGCGCCCGCCAGCCTGCG
CAAGCAAATCTGA

Domains

Predicted by InterproScan.

Sigma-54, DNA binding domain (Sigma54_DBD)

(353-508)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(133-337)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

47.868

100

0.484

Multiple sequence alignment