Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   FRE64_RS02410 Genome accession   NZ_CP042326
Coordinates   519667..520779 (-) Length   370 a.a.
NCBI ID   WP_146294502.1    Uniprot ID   -
Organism   Euhalothece natronophila Z-M001     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 514667..525779
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FRE64_RS02390 (FRE64_02390) - 514942..515568 (-) 627 WP_146294498.1 DUF6391 domain-containing protein -
  FRE64_RS02395 (FRE64_02395) - 515646..518009 (-) 2364 WP_186708927.1 type IV pilus secretin family protein -
  FRE64_RS02400 (FRE64_02400) - 518085..518876 (-) 792 WP_146294500.1 pilus assembly protein PilO -
  FRE64_RS02405 (FRE64_02405) - 518905..519651 (-) 747 WP_146294501.1 PilN domain-containing protein -
  FRE64_RS02410 (FRE64_02410) pilM 519667..520779 (-) 1113 WP_146294502.1 type IV pilus assembly protein PilM Machinery gene
  FRE64_RS02415 (FRE64_02415) - 521064..524114 (+) 3051 WP_146294503.1 UPF0182 family protein -
  FRE64_RS02420 (FRE64_02420) fabD 524210..525094 (-) 885 WP_146294504.1 ACP S-malonyltransferase -

Sequence

Protein


Download         Length: 370 a.a.        Molecular weight: 41076.69 Da        Isoelectric Point: 4.0257

>NTDB_id=377092 FRE64_RS02410 WP_146294502.1 519667..520779(-) (pilM) [Euhalothece natronophila Z-M001]
MLKFLSKLTPGFLQKKAKGVGIEITPNQINIAKVNSTEQGYELATLATKDVPEGIYTEGRIFDPPSLAELIKEALEENNI
TSQSVSTAIPMRDSIVNIIPVPAELEEDELRDVVLNQEAGLYLPYPKEEVDLDYQKLGYFADEDGIEKVQVLLIATRREV
TDLYLDIFQEQVGLNINALEINTIALIRTIREQLRQYPSQEAVILVDIEFDSTELAIIVDGVPEFNRTIPIGSFQLQSAL
SRAMNLPPSRNLEPLQEMTLPLPSDAKEQTTPFNPGMEALQRVLGELTDEVRRSADYYLNQTEGLEIVQIFLAGSGAGIG
QLDSFFYQRLNIPATQIDPVEALSLETDEEISVAQRVSLGTVLGLALREA

Nucleotide


Download         Length: 1113 bp        

>NTDB_id=377092 FRE64_RS02410 WP_146294502.1 519667..520779(-) (pilM) [Euhalothece natronophila Z-M001]
ATGTTAAAATTTTTATCAAAACTGACTCCTGGCTTTCTTCAAAAGAAGGCAAAGGGAGTAGGAATTGAAATTACACCGAA
CCAAATTAACATTGCAAAAGTAAATAGCACAGAGCAAGGTTATGAACTAGCAACCCTTGCTACTAAAGACGTTCCCGAGG
GAATTTACACTGAGGGACGAATTTTTGATCCACCTAGTCTTGCCGAATTAATTAAGGAAGCTCTAGAGGAGAACAATATC
ACCTCCCAAAGTGTTAGCACGGCGATTCCGATGCGAGACTCTATTGTCAATATTATTCCAGTACCGGCAGAACTAGAAGA
AGACGAATTAAGAGATGTGGTTCTCAATCAAGAAGCAGGGCTTTATCTCCCCTATCCTAAAGAGGAAGTGGACTTAGACT
ATCAGAAATTAGGCTACTTTGCTGATGAAGATGGGATTGAAAAAGTACAAGTGCTACTCATTGCCACACGGCGAGAAGTA
ACGGATCTCTATCTTGATATTTTTCAAGAGCAAGTCGGACTCAACATTAATGCTCTTGAGATTAACACGATCGCGCTCAT
TCGGACGATCCGAGAACAGCTGCGACAGTATCCTTCTCAAGAAGCCGTGATTTTAGTGGATATTGAGTTTGATAGCACCG
AATTGGCGATTATCGTTGATGGCGTGCCCGAGTTTAACCGCACCATTCCCATTGGGAGTTTTCAACTGCAAAGTGCCCTC
AGTCGCGCCATGAATCTTCCTCCTTCTCGGAATTTAGAGCCTTTACAAGAAATGACCTTGCCCCTACCCTCTGATGCTAA
AGAACAAACGACACCTTTTAATCCCGGGATGGAAGCATTACAGCGAGTTTTAGGGGAATTAACCGATGAAGTTCGTCGTT
CGGCAGATTATTACTTAAATCAAACTGAAGGCTTAGAAATTGTCCAAATCTTTTTAGCAGGTTCCGGAGCCGGGATTGGT
CAATTAGATAGCTTCTTTTATCAGCGTCTTAATATTCCAGCGACCCAAATTGATCCCGTTGAGGCTTTATCCTTAGAAAC
GGATGAAGAAATTTCTGTGGCTCAACGCGTCAGTTTGGGAACGGTTTTAGGGCTAGCCCTACGAGAAGCATAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(21-369)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Synechocystis sp. PCC 6803

62.331

99.73

0.622

Multiple sequence alignment