Detailed information NTG0376814

Bioinformatically predicted

Overview

• Name: pilB
• Type: Machinery gene
• Locus tag: HAV35_RS04080
• Genome accession: NZ_CP050120
• Coordinates: 783714..786377 (+)
• Length: 887 a.a.
• NCBI ID: WP_027479822.1
• Uniprot ID: -
• Organism: Deinococcus radiodurans strain BND-54
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 778714..791377

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
HAV35_RS04050 (HAV35_04050)	-	779521..780006 (+)	486	WP_010888603.1	GreA/GreB family elongation factor	-
HAV35_RS04055 (HAV35_04055)	-	780077..780427 (-)	351	WP_027479824.1	helix-turn-helix domain-containing protein	-
HAV35_RS04060 (HAV35_04060)	-	780517..781155 (+)	639	WP_027479823.1	nitroreductase family protein	-
HAV35_RS04065 (HAV35_04065)	-	781364..782158 (-)	795	WP_010888600.1	enoyl-ACP reductase	-
HAV35_RS04070 (HAV35_04070)	pgeF	782252..783013 (+)	762	WP_010888599.1	peptidoglycan editing factor PgeF	-
HAV35_RS04075 (HAV35_04075)	-	783010..783534 (+)	525	WP_010888598.1	YqeG family HAD IIIA-type phosphatase	-
HAV35_RS04080 (HAV35_04080)	pilB	783714..786377 (+)	2664	WP_027479822.1	ATPase, T2SS/T4P/T4SS family	Machinery gene
HAV35_RS04085 (HAV35_04085)	-	786440..786910 (+)	471	Protein_800	ATPase, T2SS/T4P/T4SS family	-
HAV35_RS04090 (HAV35_04090)	tnpB	786903..788108 (-)	1206	WP_231885333.1	IS200/IS605 family element RNA-guided endonuclease TnpB	-
HAV35_RS04095 (HAV35_04095)	tnpA	788116..788538 (-)	423	WP_010887312.1	IS200/IS605-like element ISDra2 family transposase	-
HAV35_RS04100 (HAV35_04100)	-	788632..789438 (+)	807	Protein_803	PilT/PilU family type 4a pilus ATPase	-
HAV35_RS04105 (HAV35_04105)	-	789501..790013 (-)	513	WP_010888595.1	hypothetical protein	-
HAV35_RS04110 (HAV35_04110)	-	790091..790774 (-)	684	WP_028328057.1	ankyrin repeat domain-containing protein	-

Sequence

Protein

• Length: 887 a.a.
• Molecular weight: 97248.06 Da
• Isoelectric Point: 4.8389

>NTDB_id=376814 HAV35_RS04080 WP_027479822.1 783714..786377(+) (pilB) [Deinococcus radiodurans strain BND-54]
MALSIGDRRLGAILLDQGYLGDNDLQRALERHSEVGGRLADVLIDSGMVGEKRIARAIEEALGIPLVNLLAVQPDPAALR
SIRPQTALNLQAFPFALEGDRLRVALVDPLSSFSIETLEDDSGFDIEPYQALREEVMWAIATHYPELGLEIVVPSGASDA
GRTGGKLGERLITHGYITDAQLQVALDAQQQTGEALGATLISQRAITEDQLYEVLAEQEGTTFLPNPSGFHPGEEVLGSM
LRADALRLLAVPVDETEQGVTVLTSDPRKRPDIDALIGRPVQLMLTRPRDIERLIEQFYPQRGRLGEQLVQEGTLSRDQL
REALQVQAREGKVKPLGEVITELGFASPDEVDSALQKQNVGGGRLEDTLVQSGKLSPEMLARSLAAQLGYEFLDPIQNPP
DPKVALMIPEATARRYVVVPVRLQGNSLVVAMKDPRNVFALDDLKLITGKEILPAVMAEKDIIRLIERYFGEKGFEKLNK
ELAERNKTQQSQEADLSVADESAIVQVVDSIIREAALQDASDIHIETTEDAVKVRYRIDGALREQNSFPKGAAQQIMARL
KIMGHLDIAERRVPQDGRVRFKKGSIDLDLRLSTLPTVYGEKAVMRLLQKASNIPELEQLGFSEYNYARYTEIIERPNGI
FLVTGPTGSGKSFTCFSTLKRIAKPEKNTTTIEDPIEYEVPGIVQSQVNNSTGMTFARALRAFLRQDPDIIFVGEIRDQE
TAKIAVEAALTGHMVLATLHTNDAPGAVTRLEEMGIENFNISAAVMGVLAQRLVRRVCSECKQPTNADPEVLRRLGISER
DIRGANLMRGTGCPRCGGTGYKGRMGIHELMVMDDSLRRTIGAGRPAAEIRDVALGESGLRSLRQDGIEKALQGLTTLEE
VLAVTAS

Nucleotide

• Length: 2664 bp

>NTDB_id=376814 HAV35_RS04080 WP_027479822.1 783714..786377(+) (pilB) [Deinococcus radiodurans strain BND-54]
TTGGCTCTTTCGATTGGTGACCGCCGCCTGGGCGCGATTTTGCTGGACCAGGGGTATCTGGGCGACAACGACTTGCAGCG
GGCGCTGGAGCGGCACTCCGAGGTGGGGGGCCGCCTCGCTGACGTGCTGATCGACTCCGGCATGGTGGGCGAAAAACGCA
TCGCCCGCGCCATCGAGGAAGCGCTCGGCATTCCGCTGGTCAACCTGCTGGCGGTGCAGCCCGACCCGGCGGCGCTGCGC
TCGATTCGGCCCCAGACGGCCCTCAACTTGCAGGCGTTTCCCTTCGCGCTCGAAGGTGACCGGCTGCGGGTGGCGCTGGT
GGACCCGCTGTCGAGCTTTTCGATCGAGACGCTTGAAGACGACAGCGGCTTCGACATCGAACCGTATCAGGCGCTGCGCG
AGGAAGTGATGTGGGCCATCGCCACGCACTACCCCGAGCTTGGCCTGGAGATCGTGGTGCCCAGCGGGGCCAGCGACGCC
GGGCGCACCGGAGGCAAGCTCGGTGAGCGGCTGATCACGCACGGCTACATCACCGACGCACAGCTTCAGGTGGCGCTCGA
CGCGCAGCAGCAGACCGGCGAGGCGCTCGGGGCGACCCTGATCTCGCAGCGGGCCATCACCGAAGACCAGCTCTACGAGG
TGCTCGCCGAACAGGAAGGCACCACCTTCCTTCCCAACCCCAGCGGTTTTCACCCCGGCGAGGAAGTCCTCGGCAGCATG
CTGCGCGCCGACGCCCTGCGCCTGCTCGCGGTGCCGGTGGACGAAACCGAGCAGGGCGTGACGGTCCTGACGAGCGACCC
GCGCAAGCGCCCCGACATCGACGCGCTCATCGGGCGCCCAGTGCAGTTGATGCTCACGCGCCCGCGCGACATCGAGCGCC
TGATTGAGCAGTTCTACCCGCAACGGGGCCGCCTCGGCGAGCAACTCGTGCAGGAAGGCACGCTGTCGCGCGACCAGTTG
CGCGAGGCGCTTCAGGTGCAGGCCCGTGAGGGCAAGGTCAAGCCGCTCGGCGAGGTCATCACCGAACTCGGATTCGCCAG
CCCCGACGAGGTGGATTCGGCGCTGCAAAAGCAGAACGTCGGCGGAGGCCGCCTGGAAGACACCCTGGTGCAGTCAGGCA
AGCTCAGCCCCGAGATGCTCGCCCGCTCGCTCGCCGCGCAGCTCGGCTACGAGTTCCTCGACCCCATCCAGAACCCGCCG
GACCCCAAGGTCGCGCTGATGATTCCCGAGGCCACCGCCCGCCGCTACGTAGTGGTGCCGGTCAGGCTCCAGGGCAACTC
GCTCGTCGTCGCCATGAAAGACCCGCGCAACGTGTTTGCGCTCGACGACCTCAAGCTGATTACCGGCAAAGAAATCCTGC
CCGCCGTGATGGCGGAAAAAGACATCATCCGCCTGATCGAGCGCTACTTCGGGGAAAAGGGCTTCGAGAAGCTCAACAAG
GAACTCGCCGAGCGCAACAAGACCCAGCAGTCGCAGGAAGCCGACCTCTCGGTGGCCGACGAGAGCGCCATCGTGCAGGT
GGTGGACTCGATTATCCGCGAGGCCGCGCTGCAAGACGCCTCGGACATTCACATCGAAACCACCGAGGACGCCGTCAAGG
TGCGCTACCGCATCGACGGTGCGCTGCGCGAGCAGAACTCCTTTCCCAAGGGCGCGGCGCAGCAGATCATGGCCCGCCTC
AAGATCATGGGCCACCTCGACATCGCCGAGCGCCGCGTGCCGCAAGACGGGCGCGTGCGCTTCAAGAAGGGCAGCATCGA
CCTCGACCTGCGTCTCTCGACCCTGCCCACCGTGTACGGCGAGAAAGCCGTCATGCGTCTGCTGCAAAAGGCGAGCAACA
TCCCCGAACTCGAGCAGCTCGGCTTTTCCGAGTACAACTACGCCCGCTACACCGAGATTATCGAGCGGCCCAACGGCATT
TTTCTGGTCACCGGGCCGACGGGGTCGGGCAAGTCGTTCACCTGTTTTTCGACCCTCAAGCGCATCGCCAAGCCCGAGAA
AAACACCACGACCATCGAAGACCCCATCGAGTACGAGGTGCCGGGCATCGTGCAGTCGCAGGTGAACAACTCGACCGGGA
TGACCTTTGCCCGCGCCCTGCGCGCCTTCCTGCGTCAGGACCCCGACATCATCTTCGTGGGCGAAATCCGTGACCAGGAA
ACCGCCAAGATTGCGGTGGAAGCGGCGCTCACCGGCCACATGGTGCTCGCCACTCTGCACACCAACGACGCGCCAGGCGC
CGTGACCCGCCTTGAGGAAATGGGCATCGAGAACTTCAACATCTCGGCGGCCGTGATGGGCGTGCTCGCGCAGCGGCTGG
TGCGCCGGGTGTGCAGCGAGTGCAAGCAGCCCACCAACGCCGACCCCGAGGTGCTCCGGCGGCTCGGCATCAGCGAGCGC
GATATTCGCGGCGCGAATCTGATGCGCGGGACCGGCTGTCCCCGCTGCGGCGGCACCGGCTACAAGGGCCGCATGGGGAT
CCACGAGCTGATGGTGATGGACGACTCGCTGCGCCGCACCATCGGGGCCGGGCGGCCCGCCGCCGAAATCCGCGACGTGG
CGCTGGGCGAAAGCGGCCTGCGGAGCCTGCGCCAGGACGGCATCGAAAAGGCCCTGCAAGGCCTGACCACCCTTGAAGAA
GTGCTGGCCGTCACCGCGAGCTGA

Domains

Predicted by InterProScan.

• Type II/IV secretion system protein (T2SSE) (511-776)

• Type II secretion system (T2SS), protein E, N-terminal domain (MshEN) (369-472)

• Type II secretion system (T2SS), protein E, N-terminal domain (MshEN) (200-303)

• Type II secretion system (T2SS), protein E, N-terminal domain (MshEN) (43-146)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilB	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	100	100	1
pilF	Thermus thermophilus HB27	56.229	100	0.565