Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   HAV35_RS02520 Genome accession   NZ_CP050120
Coordinates   512889..513842 (+) Length   317 a.a.
NCBI ID   WP_010888888.1    Uniprot ID   Q9RS67
Organism   Deinococcus radiodurans strain BND-54     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 507889..518842
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HAV35_RS02500 (HAV35_02500) - 509499..510392 (+) 894 WP_027480339.1 MBL fold metallo-hydrolase -
  HAV35_RS02505 (HAV35_02505) - 510441..511064 (+) 624 WP_010888891.1 Dps family protein -
  HAV35_RS02510 (HAV35_02510) - 511135..511689 (+) 555 WP_227085949.1 DUF4385 domain-containing protein -
  HAV35_RS02515 (HAV35_02515) - 511753..512736 (+) 984 WP_010888889.1 aldo/keto reductase -
  HAV35_RS02520 (HAV35_02520) pilA 512889..513842 (+) 954 WP_010888888.1 signal recognition particle-docking protein FtsY Machinery gene
  HAV35_RS02525 (HAV35_02525) - 513948..514196 (+) 249 WP_010888887.1 helix-turn-helix transcriptional regulator -
  HAV35_RS02530 (HAV35_02530) - 514237..514689 (+) 453 WP_010888886.1 hypothetical protein -
  HAV35_RS02535 (HAV35_02535) - 514736..516400 (+) 1665 WP_010888885.1 erythromycin esterase family protein -
  HAV35_RS02540 (HAV35_02540) recP/tkt 516472..518472 (-) 2001 WP_010888884.1 transketolase Machinery gene

Sequence

Protein


Download         Length: 317 a.a.        Molecular weight: 34321.18 Da        Isoelectric Point: 6.1587

>NTDB_id=376809 HAV35_RS02520 WP_010888888.1 512889..513842(+) (pilA) [Deinococcus radiodurans strain BND-54]
MSWFDRLRQGLSKTRQQISGTAGPLDQEVQTAFTRIDNIEDLEYALIAADVGRAATEEIIEDIRKNGEGRLQDALMRALT
LQLEPNARRAQFRELGFSPDVSRSKVDPKGHVVMVIGVNGVGKTTTIAKLGQYYMERGQSVMFAAGDTFRAAAGTQLGVW
GERLGVPVIQGVDGGDPAAVAFDAAGARKARGTDLLFVDTAGRLHNKHNLMEELKKVRRVIDKADPGEPAEVWLVLDAVT
GQNGLQQAKKFHEATPLTGVVVTKLDGTAKGGILVPIVRELGVPIKFIGVGEQPGDLQPFDSQEFVRALFDVELPKA

Nucleotide


Download         Length: 954 bp        

>NTDB_id=376809 HAV35_RS02520 WP_010888888.1 512889..513842(+) (pilA) [Deinococcus radiodurans strain BND-54]
ATGAGCTGGTTTGACCGCCTCCGCCAGGGCCTGAGCAAAACCCGGCAACAGATCAGCGGCACGGCGGGGCCGCTCGATCA
GGAGGTCCAGACAGCCTTTACCCGCATCGACAACATCGAGGACCTCGAGTACGCCCTGATTGCCGCCGACGTGGGCCGGG
CGGCCACCGAAGAAATCATCGAGGACATCCGCAAAAATGGCGAGGGCCGGCTGCAAGACGCGCTGATGCGCGCGCTCACG
CTGCAACTTGAACCCAACGCCCGGCGCGCGCAGTTCCGGGAACTCGGCTTTTCGCCGGATGTCAGCCGCTCCAAGGTGGA
CCCCAAGGGCCACGTCGTCATGGTCATCGGCGTGAACGGCGTGGGCAAGACCACCACCATCGCTAAGCTCGGCCAGTACT
ACATGGAGCGCGGCCAGAGCGTCATGTTTGCGGCGGGCGACACCTTCCGCGCGGCGGCGGGCACGCAGCTCGGCGTGTGG
GGCGAGCGCCTCGGCGTGCCGGTCATTCAGGGTGTGGACGGCGGCGACCCGGCGGCGGTGGCCTTCGACGCGGCGGGTGC
CCGCAAAGCACGCGGCACCGACCTGCTGTTCGTGGACACGGCGGGGCGACTGCACAATAAGCACAACCTGATGGAAGAGT
TGAAAAAGGTCCGCCGCGTCATCGACAAGGCCGACCCCGGCGAGCCCGCCGAGGTGTGGCTGGTGCTCGACGCTGTGACC
GGCCAGAACGGGCTGCAACAGGCCAAGAAGTTCCACGAGGCCACTCCGCTGACCGGCGTGGTCGTCACCAAGCTCGACGG
CACCGCCAAGGGCGGCATCCTCGTGCCCATCGTCCGCGAACTCGGCGTGCCCATCAAATTCATCGGCGTGGGCGAGCAGC
CCGGCGACCTGCAACCGTTCGACAGCCAGGAGTTCGTGCGGGCACTGTTTGACGTGGAGCTGCCCAAAGCCTGA

Domains

Predicted by InterProScan.

SRP54-type protein, helical bundle domain (SRP54_N)

(37-69)

SRP54-type protein, GTPase domain (SRP54)

(111-310)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q9RS67

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Neisseria gonorrhoeae MS11

45.768

100

0.461

Multiple sequence alignment