Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   FRC75_RS18660 Genome accession   NZ_CP042302
Coordinates   4048991..4050580 (-) Length   529 a.a.
NCBI ID   WP_011793602.1    Uniprot ID   A1TJ88
Organism   Paracidovorax citrulli strain NWB SC074     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4043991..4055580
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FRC75_RS18640 (FRC75_18700) - 4044555..4045037 (-) 483 WP_011793606.1 putative toxin-antitoxin system toxin component, PIN family -
  FRC75_RS18645 (FRC75_18705) - 4045027..4046352 (-) 1326 WP_011793605.1 THUMP domain-containing protein -
  FRC75_RS18650 (FRC75_18710) - 4046507..4048363 (+) 1857 WP_011793604.1 methyl-accepting chemotaxis protein -
  FRC75_RS18655 (FRC75_18715) - 4048421..4048966 (+) 546 WP_116212457.1 TspO/MBR family protein -
  FRC75_RS18660 (FRC75_18720) rpoN/rpoN1 4048991..4050580 (-) 1590 WP_011793602.1 RNA polymerase factor sigma-54 Machinery gene
  FRC75_RS18665 (FRC75_18725) lptB 4050580..4051371 (-) 792 WP_011793601.1 LPS export ABC transporter ATP-binding protein -
  FRC75_RS18670 (FRC75_18730) lptA 4051374..4052024 (-) 651 WP_011793600.1 lipopolysaccharide transport periplasmic protein LptA -
  FRC75_RS18675 (FRC75_18735) - 4052144..4052800 (-) 657 WP_011793599.1 thiol:disulfide interchange protein DsbA/DsbL -
  FRC75_RS18680 (FRC75_18740) - 4052870..4053517 (-) 648 WP_041827195.1 SPOR domain-containing protein -
  FRC75_RS18685 (FRC75_18745) argS 4053523..4055232 (-) 1710 WP_011793597.1 arginine--tRNA ligase -

Sequence

Protein


Download         Length: 529 a.a.        Molecular weight: 57887.10 Da        Isoelectric Point: 4.6032

>NTDB_id=376770 FRC75_RS18660 WP_011793602.1 4048991..4050580(-) (rpoN/rpoN1) [Paracidovorax citrulli strain NWB SC074]
MKPGLSLRISQHLALTPQLQQSIRLLQLSTLELSQEVEQMLDENPFLERNPDEAPREEFGLLQADAPVSDADRGAEESLY
SPASGAVETAASAPGEGASEGSEAPEAPDAPDWEGDGTVEMAPDDGEWGGEAPARTRNQLGDEDDTDATELAREHESLTD
YLHRQALSLRLSEVDRAALRFLIESLNDDGYLDESLEALAVALAGEEDPEQVEELVHRFTVALRLLQSLEPVGVGARGLS
ECLALQLRALQEDGDAEPEVLQAALRICNQPLEMLARRDVRRLAQGCGAGEECTRAAMALIARLEPRPGRAFAQVERNII
VPDVIVKKSSGRGAAHAFTVQLNPDVMPRLRVHDIYAGALRGNRGGEGHQALQQRLQEARWFIKNIQQRFDTILRVSRAI
VDRQKNFFVHGELAMRPLVLRDIADELGLHESTISRVTTAKYMATPQGTYELKYFFGSGLGTETGGNASSTAVRALIKQF
VASESPAKPLSDSQIAEMLKEQGIECARRTVAKYREALKIAPANLRKAL

Nucleotide


Download         Length: 1590 bp        

>NTDB_id=376770 FRC75_RS18660 WP_011793602.1 4048991..4050580(-) (rpoN/rpoN1) [Paracidovorax citrulli strain NWB SC074]
ATGAAGCCGGGACTGTCGCTGCGCATCTCGCAGCATCTGGCGCTGACGCCGCAACTGCAGCAATCGATCCGGCTGCTGCA
GCTGTCCACGCTGGAGCTGTCGCAGGAAGTCGAGCAGATGCTCGACGAGAACCCCTTCCTGGAGCGCAACCCGGACGAGG
CACCGCGCGAGGAGTTCGGGCTGCTGCAGGCGGATGCGCCGGTGAGCGACGCCGACCGCGGGGCGGAGGAGTCCCTCTAT
TCGCCGGCCAGCGGGGCGGTGGAGACGGCGGCCTCGGCGCCGGGCGAGGGCGCATCCGAAGGATCGGAGGCGCCGGAAGC
TCCCGATGCGCCGGACTGGGAGGGCGACGGCACCGTCGAGATGGCGCCCGACGACGGCGAGTGGGGCGGCGAGGCGCCCG
CGCGCACGCGCAACCAGCTGGGGGACGAAGACGACACGGACGCGACCGAACTCGCGCGCGAGCACGAGTCGCTCACCGAT
TACCTGCACCGGCAGGCGCTGTCGCTGCGCCTGTCGGAGGTGGACCGCGCGGCGCTGCGCTTCCTGATCGAATCGCTGAA
CGACGACGGCTACCTCGACGAATCGCTGGAGGCACTGGCCGTGGCCCTCGCGGGCGAGGAAGACCCCGAGCAGGTGGAGG
AACTGGTGCATCGCTTCACGGTGGCGCTGCGGCTGCTGCAGAGCCTGGAGCCCGTGGGCGTGGGCGCGCGCGGCCTGAGC
GAATGCCTGGCGCTGCAGCTGCGGGCCCTGCAGGAAGATGGCGACGCGGAGCCCGAAGTGCTGCAGGCGGCGCTGCGCAT
CTGCAATCAGCCGCTGGAGATGCTGGCGCGGCGCGACGTGCGCCGGCTCGCGCAGGGTTGCGGTGCCGGCGAGGAGTGCA
CGCGCGCGGCGATGGCGCTCATCGCGCGGCTGGAGCCCCGGCCGGGCCGGGCTTTCGCGCAGGTCGAGCGCAACATCATC
GTGCCCGACGTGATCGTGAAGAAGAGCAGTGGCCGCGGGGCCGCGCATGCGTTCACCGTGCAGCTCAACCCGGATGTCAT
GCCGCGGCTGCGCGTGCACGACATCTATGCCGGGGCGCTGCGCGGCAACCGCGGCGGCGAGGGGCACCAGGCGCTGCAGC
AGCGGCTGCAGGAAGCGCGTTGGTTCATCAAGAACATCCAGCAGCGGTTCGACACCATCCTGCGCGTCTCGCGCGCCATC
GTGGACCGGCAGAAGAACTTCTTCGTGCATGGCGAGCTGGCCATGCGGCCGCTCGTGCTGCGCGACATCGCCGACGAACT
GGGGCTGCACGAATCGACGATCAGCCGGGTGACCACGGCCAAGTACATGGCCACGCCCCAGGGTACCTACGAGCTGAAGT
ATTTCTTCGGCTCGGGCCTGGGCACGGAAACGGGCGGCAATGCGTCGAGCACGGCCGTGCGTGCGCTGATCAAGCAGTTC
GTCGCGTCGGAGAGCCCGGCCAAGCCGCTGTCGGACAGCCAGATCGCGGAGATGCTCAAGGAGCAGGGCATCGAATGCGC
GCGCCGCACGGTGGCGAAATACCGCGAGGCGCTGAAGATCGCACCGGCCAACCTGCGCAAGGCGCTGTAA

Domains

Predicted by InterproScan.

Sigma-54 factor, core binding domain (Sigma54_CBD)

(149-356)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(371-527)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A1TJ88

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

52.731

100

0.529

Multiple sequence alignment