Detailed information NTG0376735

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: FKV26_RS10350
• Genome accession: NZ_CP042299
• Coordinates: 2304260..2305276 (-)
• Length: 338 a.a.
• NCBI ID: WP_000520444.1
• Uniprot ID: A0A0F0B833
• Organism: Vibrio cholerae O1 strain AAS91
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2299260..2310276

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
FKV26_RS10325 (FKV26_10605)	aroK	2300143..2300667 (-)	525	WP_000818616.1	shikimate kinase AroK	-
FKV26_RS10330 (FKV26_10610)	pilQ	2300854..2302569 (-)	1716	WP_000788426.1	type IV pilus secretin PilQ family protein	Machinery gene
FKV26_RS10335 (FKV26_10615)	pilP	2302595..2303113 (-)	519	WP_000792032.1	pilus assembly protein PilP	Machinery gene
FKV26_RS10340 (FKV26_10620)	pilO	2303103..2303693 (-)	591	WP_000157690.1	type 4a pilus biogenesis protein PilO	Machinery gene
FKV26_RS10345 (FKV26_10625)	pilN	2303686..2304270 (-)	585	WP_000902745.1	PilN domain-containing protein	Machinery gene
FKV26_RS10350 (FKV26_10630)	pilM	2304260..2305276 (-)	1017	WP_000520444.1	type IV pilus assembly protein PilM	Machinery gene
FKV26_RS10355 (FKV26_10635)	-	2305424..2307901 (+)	2478	WP_000668743.1	penicillin-binding protein 1A	-
FKV26_RS10360 (FKV26_10640)	oxyR	2307977..2308870 (-)	894	WP_001025909.1	DNA-binding transcriptional regulator OxyR	-
FKV26_RS10365 (FKV26_10645)	-	2309010..2309741 (+)	732	WP_000495223.1	glutathione peroxidase	-

Sequence

Protein

• Length: 338 a.a.
• Molecular weight: 37707.44 Da
• Isoelectric Point: 7.3328

>NTDB_id=376735 FKV26_RS10350 WP_000520444.1 2304260..2305276(-) (pilM) [Vibrio cholerae O1 strain AAS91]
MGKSLVTGIDIGHHSIKAAVLKPMGDTYALVGYEELLVTADIFTDNHTLDYQKIVKKLKELKKGLPLFSHKVAIAIPDSA
VISKVLQIDSDLEQRELEFAVYQAFSHQSPFPVEELSLDFVKVTEKSLARSSTTTFQVYATKKDVVDSRLHAVKKAGFEP
VLMDVQVHSLLHLWQLASRAYRRPDWMLVDIGYTQSSLCLDFPEKMPFYKDVPLGTRQLESEPSAVNSLNHPLLHDPTQR
FIQELVDKIARQIQLFTSVHGTQSLGGLWLSGGGATLSGLEDALYQRLSLPCEVLNPFSLFKNNVAKRKRPLVDGQRFST
AAGLALRGLAWLESEHVA

Nucleotide

• Length: 1017 bp

>NTDB_id=376735 FKV26_RS10350 WP_000520444.1 2304260..2305276(-) (pilM) [Vibrio cholerae O1 strain AAS91]
ATGGGTAAATCATTAGTTACGGGTATTGATATCGGCCACCACAGCATTAAAGCTGCGGTGCTCAAACCTATGGGCGACAC
TTATGCGCTTGTGGGGTATGAGGAGTTGCTCGTTACCGCTGATATTTTCACCGATAATCACACGCTCGATTATCAGAAAA
TTGTAAAGAAACTAAAAGAACTAAAAAAGGGTTTGCCTCTATTTAGTCATAAAGTCGCGATAGCAATCCCGGATAGCGCC
GTGATTAGCAAAGTATTACAAATAGATAGCGATCTTGAGCAGCGCGAGCTGGAATTTGCTGTCTATCAAGCCTTTTCGCA
TCAATCTCCATTCCCCGTTGAAGAGCTGAGCCTCGATTTCGTCAAAGTGACGGAAAAAAGCCTCGCCCGCAGCAGCACGA
CGACCTTCCAAGTGTATGCCACCAAAAAAGACGTGGTCGACAGTCGTTTGCATGCCGTTAAAAAAGCGGGTTTTGAACCA
GTGTTGATGGATGTGCAAGTACATAGTTTGTTGCATTTATGGCAACTCGCTAGCCGTGCGTATCGACGTCCAGACTGGAT
GTTGGTGGATATTGGCTACACCCAAAGTTCATTGTGCCTCGATTTTCCGGAAAAAATGCCCTTTTACAAAGACGTGCCAT
TAGGAACTCGTCAATTGGAATCAGAACCCAGTGCTGTGAACTCATTGAATCATCCACTGTTACACGATCCCACGCAGCGG
TTTATTCAAGAATTGGTCGACAAAATTGCGCGGCAAATCCAGCTTTTTACTTCGGTGCACGGGACTCAAAGTCTTGGCGG
GTTATGGCTATCGGGTGGCGGTGCCACGCTTTCTGGTTTAGAGGACGCGCTTTATCAACGCTTGTCACTGCCTTGCGAAG
TGCTCAATCCGTTTTCACTGTTCAAAAACAATGTGGCAAAACGCAAACGCCCACTGGTTGATGGCCAGCGTTTTAGTACC
GCCGCCGGTCTTGCATTGCGAGGCTTAGCTTGGTTGGAGAGTGAGCATGTTGCATAA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (8-221)

• Type IV pilus assembly protein PilM; (PilM_2) (241-328)

3D structure

• AlphaFold DB: A0A0F0B833

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Vibrio cholerae strain A1552	100	100	1
pilM	Vibrio campbellii strain DS40M4	48.521	100	0.485