Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   G9P52_RS10585 Genome accession   NZ_CP050047
Coordinates   2175185..2175625 (+) Length   146 a.a.
NCBI ID   WP_000360895.1    Uniprot ID   B7LFX5
Organism   Escherichia coli strain 90-1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2170185..2180625
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  G9P52_RS10565 (G9P52_10560) aroP 2171183..2172556 (+) 1374 WP_000969915.1 aromatic amino acid transporter AroP -
  G9P52_RS10570 (G9P52_10565) ampE 2172599..2173453 (-) 855 WP_000172005.1 beta-lactamase regulator AmpE -
  G9P52_RS10575 (G9P52_10570) ampD 2173450..2174001 (-) 552 WP_000923721.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  G9P52_RS10580 (G9P52_10575) nadC 2174089..2174982 (+) 894 WP_001135168.1 carboxylating nicotinate-nucleotide diphosphorylase -
  G9P52_RS10585 (G9P52_10580) pilA 2175185..2175625 (+) 441 WP_000360895.1 prepilin peptidase-dependent pilin Machinery gene
  G9P52_RS10590 (G9P52_10585) pilB 2175635..2177020 (+) 1386 WP_001025171.1 type II secretion system protein GspE Machinery gene
  G9P52_RS10595 (G9P52_10590) hofC 2177010..2178212 (+) 1203 WP_000157229.1 protein transport protein HofC -
  G9P52_RS10600 (G9P52_10595) guaC 2178247..2179290 (-) 1044 WP_001217338.1 GMP reductase -
  G9P52_RS10605 - 2179446..2179490 (-) 45 WP_120795372.1 protein YacM -
  G9P52_RS10610 (G9P52_10600) coaE 2179515..2180135 (+) 621 WP_001269520.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15602.71 Da        Isoelectric Point: 4.3927

>NTDB_id=376396 G9P52_RS10585 WP_000360895.1 2175185..2175625(+) (pilA) [Escherichia coli strain 90-1]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTH
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=376396 G9P52_RS10585 WP_000360895.1 2175185..2175625(+) (pilA) [Escherichia coli strain 90-1]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAGATACCTGCGACGGTGGCAGCAATGGCATTCCCTCGCCTACCACCACCCAC
TATGTTTCAGCCATGAGTGTGGCAAAAGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGACTAAGCGTCGTCAT
GACACCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGCAACTGCAATATTCAAAGTGACAGCGCATTGCAGC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B7LFX5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

43.089

84.247

0.363