Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   FP515_RS00535 Genome accession   NZ_CP042251
Coordinates   100940..102313 (+) Length   457 a.a.
NCBI ID   WP_011229594.1    Uniprot ID   A0A7U9ERF5
Organism   Geobacillus thermoleovorans strain ARTRW1     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 95940..107313
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FP515_RS00515 (FP515_00515) ctsR 96311..96772 (+) 462 WP_011229590.1 transcriptional regulator CtsR -
  FP515_RS00520 (FP515_00520) - 96788..97336 (+) 549 WP_011229591.1 UvrB/UvrC motif-containing protein -
  FP515_RS00525 (FP515_00525) - 97342..98433 (+) 1092 WP_011229592.1 protein arginine kinase -
  FP515_RS00530 (FP515_00530) clpC 98430..100862 (+) 2433 WP_011229593.1 ATP-dependent protease ATP-binding subunit ClpC -
  FP515_RS00535 (FP515_00535) radA 100940..102313 (+) 1374 WP_011229594.1 DNA repair protein RadA Machinery gene
  FP515_RS00540 (FP515_00540) - 102623..103717 (+) 1095 WP_011229595.1 PIN/TRAM domain-containing protein -
  FP515_RS00545 (FP515_00545) ispD 103739..104425 (+) 687 WP_011229596.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  FP515_RS00550 (FP515_00550) ispF 104440..104916 (+) 477 WP_011229597.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -
  FP515_RS00555 (FP515_00555) gltX 105119..106591 (+) 1473 WP_011229598.1 glutamate--tRNA ligase -

Sequence

Protein


Download         Length: 457 a.a.        Molecular weight: 49479.10 Da        Isoelectric Point: 7.6467

>NTDB_id=376204 FP515_RS00535 WP_011229594.1 100940..102313(+) (radA) [Geobacillus thermoleovorans strain ARTRW1]
MAKKKTKFVCQECGYESAKWLGRCPGCQTWNSFVEEIEQEKPTVRGAFLHSERAGSAKPVPITAVEAVQEPRMKTNSAEL
DRVLGGGIVKGSLVLIGGDPGIGKSTLLLQTSAQLAAAGHQVLYVSGEESVKQVKLRAGRLRAECDQLYVLAEADLEYIV
AAVETVQPACVIIDSIQTVYRTDISSAPGSVAQVRECTAELMKLAKTKGIAIFIVGHVTKEGAIAGPRLLEHMVDTVLYF
EGERHHTYRILRAVKNRFGSTNEIGIFEMRDIGLKEVENPSEVFLEERSRGAAGSTVVAAMEGTRPVLVEIQALVSPTSF
GNPRRMATGLDHNRVSLLMAVLEKRVGLLLQNQDAYLKVAGGVKLDEPAIDLAVAVSIASSFRDRPTNPADVIIGEVGLT
GEVRRVSRIEQRVQEAVKLGFSRIIVPKNNLAGWQPPKGVQVIGVSHVAEALEHTMC

Nucleotide


Download         Length: 1374 bp        

>NTDB_id=376204 FP515_RS00535 WP_011229594.1 100940..102313(+) (radA) [Geobacillus thermoleovorans strain ARTRW1]
ATGGCGAAGAAGAAAACGAAGTTCGTTTGCCAAGAGTGTGGATATGAGTCGGCCAAATGGCTTGGACGCTGTCCGGGATG
CCAAACATGGAATTCGTTTGTTGAGGAAATTGAGCAGGAGAAACCGACTGTGCGCGGCGCATTTCTTCATTCGGAGCGGG
CGGGTTCGGCCAAACCGGTCCCGATTACAGCCGTGGAGGCGGTGCAAGAGCCGCGCATGAAAACGAACAGCGCGGAGCTC
GATCGCGTGCTGGGCGGTGGAATCGTCAAAGGTTCGCTCGTTTTGATCGGCGGCGACCCTGGCATTGGCAAATCAACGTT
GCTTTTGCAAACATCGGCGCAGCTGGCCGCCGCTGGGCATCAAGTGCTGTATGTCTCCGGTGAGGAATCGGTCAAACAAG
TAAAGCTGCGCGCTGGACGCCTTCGTGCGGAGTGCGATCAGCTGTACGTGTTGGCGGAAGCAGATTTGGAATACATTGTG
GCAGCGGTTGAAACGGTTCAACCTGCTTGTGTCATCATTGATTCGATTCAGACGGTGTATCGCACCGATATTTCCTCTGC
ACCGGGCAGCGTCGCCCAAGTGCGCGAATGCACGGCCGAGCTGATGAAGCTGGCGAAAACAAAAGGCATTGCCATTTTTA
TCGTCGGTCATGTGACGAAGGAAGGGGCGATCGCCGGGCCGCGCCTGCTCGAACATATGGTGGACACCGTTCTCTATTTT
GAAGGGGAGCGACACCATACGTATCGCATTTTGCGAGCGGTGAAAAACCGTTTTGGCTCAACGAACGAAATCGGCATTTT
TGAGATGCGCGACATTGGGCTGAAGGAAGTGGAAAACCCATCAGAAGTGTTTTTAGAAGAACGGTCGCGCGGAGCGGCTG
GCTCGACGGTCGTGGCGGCGATGGAAGGGACGCGGCCGGTGCTCGTGGAAATCCAGGCGCTCGTCTCGCCGACGAGCTTC
GGCAACCCGAGACGGATGGCGACCGGCCTTGACCATAACCGTGTGTCGCTTTTGATGGCCGTGCTCGAAAAGCGGGTTGG
GCTGCTGTTGCAAAACCAAGATGCTTATTTGAAAGTGGCGGGCGGCGTGAAGCTTGATGAGCCGGCAATTGACTTGGCGG
TCGCTGTCAGCATCGCCTCGAGCTTTCGCGATCGGCCGACGAATCCGGCGGATGTCATTATCGGCGAAGTCGGCTTAACT
GGAGAGGTGCGCCGCGTCTCGCGCATCGAACAGCGCGTGCAGGAGGCGGTAAAATTAGGCTTTTCCCGCATCATTGTGCC
AAAAAACAATTTAGCCGGCTGGCAGCCGCCAAAAGGTGTGCAAGTGATCGGTGTTTCGCATGTGGCCGAGGCGCTTGAGC
ATACAATGTGTTAA

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(68-222)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

Subunit ChlI of Mg-chelatase (ChlI)

(365-430)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7U9ERF5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

73.961

100

0.74
  radA Streptococcus mitis NCTC 12261

65.784

99.125

0.652
  radA Streptococcus mitis SK321

65.784

99.125

0.652
  radA Streptococcus pneumoniae Rx1

65.784

99.125

0.652
  radA Streptococcus pneumoniae D39

65.784

99.125

0.652
  radA Streptococcus pneumoniae R6

65.784

99.125

0.652
  radA Streptococcus pneumoniae TIGR4

65.784

99.125

0.652

Multiple sequence alignment