Detailed information    

insolico Bioinformatically predicted

Overview


Name   nucA/comI   Type   Machinery gene
Locus tag   FPS98_RS07025 Genome accession   NZ_CP042161
Coordinates   1476405..1476833 (-) Length   142 a.a.
NCBI ID   WP_144614739.1    Uniprot ID   A0A517I4I4
Organism   Brevibacillus brevis strain HK544     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Related MGE


Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 1451448..1481599 1476405..1476833 within 0


Gene organization within MGE regions


Location: 1451448..1481599
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FPS98_RS06875 (FPS98_06875) - 1451448..1452698 (+) 1251 WP_144614705.1 hypothetical protein -
  FPS98_RS06880 (FPS98_06880) - 1452768..1453076 (+) 309 WP_144614707.1 hypothetical protein -
  FPS98_RS06890 (FPS98_06890) - 1453833..1454666 (+) 834 WP_261376213.1 RNA ligase family protein -
  FPS98_RS06895 (FPS98_06895) hflX 1454753..1456045 (+) 1293 WP_144614711.1 GTPase HflX -
  FPS98_RS06900 (FPS98_06900) - 1456053..1457330 (+) 1278 WP_144614713.1 aminotransferase class I/II-fold pyridoxal phosphate-dependent enzyme -
  FPS98_RS06905 (FPS98_06905) - 1457500..1457910 (+) 411 WP_015891641.1 MerR family transcriptional regulator -
  FPS98_RS06910 (FPS98_06910) glnA 1457946..1459280 (+) 1335 WP_007719084.1 type I glutamate--ammonia ligase -
  FPS98_RS31320 - 1459454..1459657 (+) 204 Protein_1361 tyrosine-type recombinase/integrase -
  FPS98_RS06920 (FPS98_06920) - 1460516..1460899 (-) 384 WP_144614717.1 hypothetical protein -
  FPS98_RS30785 - 1461043..1461435 (+) 393 WP_186380560.1 hypothetical protein -
  FPS98_RS06930 (FPS98_06930) - 1461513..1461848 (-) 336 WP_144614718.1 DeoR family transcriptional regulator -
  FPS98_RS30790 - 1461879..1462043 (+) 165 WP_186380561.1 hypothetical protein -
  FPS98_RS06935 (FPS98_06935) - 1462108..1462749 (-) 642 WP_144614719.1 hypothetical protein -
  FPS98_RS06940 (FPS98_06940) - 1462938..1463477 (-) 540 WP_144614721.1 hypothetical protein -
  FPS98_RS31800 - 1465325..1465504 (+) 180 WP_409995172.1 DNA adenine methylase -
  FPS98_RS31325 - 1465687..1465968 (+) 282 WP_261376269.1 hypothetical protein -
  FPS98_RS06960 (FPS98_06960) - 1466023..1466481 (-) 459 WP_144614723.1 hypothetical protein -
  FPS98_RS06965 (FPS98_06965) - 1467030..1467722 (+) 693 WP_144614725.1 hypothetical protein -
  FPS98_RS06970 (FPS98_06970) - 1468447..1468704 (+) 258 WP_261376127.1 hypothetical protein -
  FPS98_RS06975 (FPS98_06975) - 1469253..1470074 (-) 822 WP_144614727.1 DUF6492 family protein -
  FPS98_RS06980 (FPS98_06980) - 1470849..1471046 (+) 198 WP_261376128.1 hypothetical protein -
  FPS98_RS06985 (FPS98_06985) - 1471248..1471712 (-) 465 WP_144614729.1 hypothetical protein -
  FPS98_RS06990 (FPS98_06990) - 1472244..1472526 (+) 283 Protein_1376 SOS response-associated peptidase family protein -
  FPS98_RS06995 (FPS98_06995) arsR 1472643..1472957 (+) 315 WP_144614731.1 arsenical resistance operon transcriptional regulator ArsR -
  FPS98_RS07000 (FPS98_07000) - 1473023..1473460 (+) 438 WP_015891623.1 ArsI/CadI family heavy metal resistance metalloenzyme -
  FPS98_RS07005 (FPS98_07005) arsB 1473479..1474522 (+) 1044 WP_144614733.1 ACR3 family arsenite efflux transporter -
  FPS98_RS07010 (FPS98_07010) arsC 1474544..1474948 (+) 405 WP_144614735.1 arsenate reductase (thioredoxin) -
  FPS98_RS07015 (FPS98_07015) - 1475252..1475890 (-) 639 WP_144614737.1 hypothetical protein -
  FPS98_RS31705 - 1476136..1476234 (+) 99 WP_315852074.1 hypothetical protein -
  FPS98_RS07025 (FPS98_07025) nucA/comI 1476405..1476833 (-) 429 WP_144614739.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  FPS98_RS31330 - 1477293..1477466 (+) 174 WP_261376129.1 hypothetical protein -
  FPS98_RS07035 (FPS98_07035) - 1477594..1477971 (+) 378 WP_144614741.1 PH domain-containing protein -
  FPS98_RS07045 (FPS98_07045) - 1478477..1478677 (+) 201 WP_144614745.1 hypothetical protein -
  FPS98_RS07050 (FPS98_07050) - 1479243..1480352 (-) 1110 WP_144614747.1 acyltransferase family protein -
  FPS98_RS07055 (FPS98_07055) - 1480895..1481599 (+) 705 WP_144614749.1 SOS response-associated peptidase -

Sequence


Protein


Download         Length: 142 a.a.        Molecular weight: 15640.99 Da        Isoelectric Point: 7.8452

>NTDB_id=375810 FPS98_RS07025 WP_144614739.1 1476405..1476833(-) (nucA/comI) [Brevibacillus brevis strain HK544]
MTQRKMLMLIVVLLIGAAYLFGLVPIEKQTVSNGNVDHTIVFPSDRYPETAKHIKEAVASGKSAVCTIDRDGADKNREKS
LKGIPTKKGYDRDEWPMAMCAEGGTGADIKYIKPSDNRGAESWISNQLEDFPNGTKVEIVIK

Nucleotide


Download         Length: 429 bp        

>NTDB_id=375810 FPS98_RS07025 WP_144614739.1 1476405..1476833(-) (nucA/comI) [Brevibacillus brevis strain HK544]
ATGACACAGAGAAAAATGCTGATGTTGATTGTGGTGCTGCTGATCGGCGCCGCCTATCTCTTTGGACTTGTTCCAATAGA
GAAGCAAACAGTCAGCAATGGGAACGTAGACCATACGATTGTTTTTCCGTCTGATCGATACCCAGAGACTGCAAAGCATA
TAAAAGAAGCGGTTGCTTCCGGGAAGTCAGCTGTATGCACAATTGACCGCGATGGAGCCGATAAAAATCGCGAAAAATCG
CTCAAGGGTATTCCTACAAAAAAGGGGTATGATCGGGATGAATGGCCAATGGCCATGTGTGCAGAGGGTGGCACAGGGGC
AGATATCAAATACATAAAGCCTTCGGATAATCGCGGCGCTGAATCTTGGATATCCAACCAATTAGAGGATTTTCCAAACG
GGACAAAGGTTGAAATCGTGATCAAATAA


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure
  AlphaFold DB A0A517I4I4

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

60

84.507

0.507


Multiple sequence alignment