Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   FP477_RS12645 Genome accession   NZ_CP042046
Coordinates   2496129..2497103 (-) Length   324 a.a.
NCBI ID   WP_000697217.1    Uniprot ID   A0AAN2D5C1
Organism   Staphylococcus aureus strain B2-7A     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2491129..2502103
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FP477_RS12605 (FP477_12605) gcvT 2491630..2492721 (-) 1092 WP_000093349.1 glycine cleavage system aminomethyltransferase GcvT -
  FP477_RS12610 (FP477_12610) - 2492880..2493404 (-) 525 WP_001015120.1 shikimate kinase -
  FP477_RS12615 (FP477_12615) - 2493394..2493540 (-) 147 WP_001792109.1 hypothetical protein -
  FP477_RS12620 (FP477_12620) comGF 2493637..2494134 (-) 498 WP_001788897.1 competence type IV pilus minor pilin ComGF Machinery gene
  FP477_RS12625 (FP477_12625) comGE 2494052..2494351 (-) 300 WP_000844410.1 hypothetical protein Machinery gene
  FP477_RS12630 (FP477_12630) comGD 2494338..2494784 (-) 447 WP_001788899.1 competence type IV pilus minor pilin ComGD Machinery gene
  FP477_RS12635 (FP477_12635) comGC 2494762..2495073 (-) 312 WP_000472256.1 competence type IV pilus major pilin ComGC Machinery gene
  FP477_RS12640 (FP477_12640) comGB 2495087..2496157 (-) 1071 WP_000776422.1 competence type IV pilus assembly protein ComGB Machinery gene
  FP477_RS12645 (FP477_12645) comGA 2496129..2497103 (-) 975 WP_000697217.1 competence type IV pilus ATPase ComGA Machinery gene
  FP477_RS12650 (FP477_12650) - 2497155..2497778 (-) 624 WP_001223008.1 MBL fold metallo-hydrolase -
  FP477_RS12655 (FP477_12655) - 2497775..2498104 (-) 330 WP_001018871.1 MTH1187 family thiamine-binding protein -
  FP477_RS12660 (FP477_12660) - 2498104..2499090 (-) 987 WP_000161314.1 ROK family glucokinase -
  FP477_RS12665 (FP477_12665) - 2499087..2499290 (-) 204 WP_000087561.1 YqgQ family protein -
  FP477_RS12670 (FP477_12670) - 2499271..2500734 (-) 1464 WP_001017865.1 rhomboid family protein -
  FP477_RS12675 (FP477_12675) - 2500746..2501285 (-) 540 WP_000164546.1 5-formyltetrahydrofolate cyclo-ligase -
  FP477_RS12680 (FP477_12680) rpmG 2501494..2501643 (-) 150 WP_001265709.1 50S ribosomal protein L33 -

Sequence

Protein


Download         Length: 324 a.a.        Molecular weight: 36869.90 Da        Isoelectric Point: 8.8516

>NTDB_id=375667 FP477_RS12645 WP_000697217.1 2496129..2497103(-) (comGA) [Staphylococcus aureus strain B2-7A]
MKILFQEIINKAIEMKASDVHFIPVKNEVSIKFRINDNLEQYEQIGNSIYQKLLVYMKFQAGLDVSTQQVAQSGRYSYHF
NKIYFLRISTLPLSLGQESCVIRIAPQFFQQQKSTYKFNDFKHLMNKKQGLLLFSGPTGSGKSTLMYQMVSYANKALNLN
VISIEDPVEMQIPGIVQINVNDKAGINYVNSFKAILRCDPDVILIGEIRDKDVAKCVIQASLSGHLVLTTLHATDCKGAI
LRLLEMGISVQELIQATNLIINQRLVTTIKQQRQLVCEILSQQQLRYFFSHNHSLPSSFKNLEDKLDDMTKAGVICETTM
DKYI

Nucleotide


Download         Length: 975 bp        

>NTDB_id=375667 FP477_RS12645 WP_000697217.1 2496129..2497103(-) (comGA) [Staphylococcus aureus strain B2-7A]
TTGAAGATTCTATTTCAAGAAATAATTAATAAAGCGATAGAAATGAAAGCGAGTGATGTACATTTTATTCCAGTTAAAAA
TGAAGTAAGTATTAAATTTAGAATTAATGATAACTTGGAGCAGTATGAACAAATTGGGAATAGCATTTATCAAAAGTTAT
TAGTTTATATGAAGTTTCAAGCTGGGCTTGATGTTTCTACACAGCAAGTCGCACAGAGCGGTCGATATAGTTACCATTTC
AATAAAATATATTTTTTGAGAATATCAACTTTACCATTGTCACTTGGCCAAGAAAGTTGTGTTATCAGAATTGCACCTCA
ATTTTTTCAACAACAGAAATCAACTTATAAATTCAATGATTTTAAACACCTCATGAATAAGAAACAAGGATTACTATTGT
TTAGTGGGCCAACTGGTTCAGGAAAGAGTACATTAATGTATCAAATGGTCTCATACGCGAATAAAGCCTTGAATTTAAAT
GTAATTTCTATAGAGGATCCTGTAGAGATGCAAATTCCTGGTATCGTCCAAATTAATGTGAATGATAAAGCTGGCATTAA
CTATGTAAATTCGTTTAAAGCTATTTTAAGATGTGATCCTGATGTTATTTTAATAGGTGAAATCAGAGATAAAGATGTTG
CCAAGTGTGTTATACAGGCTAGTTTAAGTGGTCACCTGGTTCTGACTACATTGCATGCAACTGATTGTAAAGGTGCTATT
TTAAGGCTATTAGAAATGGGCATTTCTGTACAAGAATTGATACAGGCAACTAATTTAATTATAAACCAACGACTTGTAAC
TACTATTAAGCAACAGCGACAATTAGTATGTGAAATTCTATCTCAGCAACAACTCCGATATTTCTTTTCCCATAATCATT
CATTACCATCATCATTTAAGAACTTAGAAGATAAACTTGATGATATGACAAAAGCAGGTGTCATTTGTGAAACTACAATG
GATAAATACATTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(7-267)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Staphylococcus aureus MW2

99.691

100

0.997
  comGA Staphylococcus aureus N315

99.691

100

0.997

Multiple sequence alignment