Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   G9W35_RS08775 Genome accession   NZ_CP049845
Coordinates   1827715..1829208 (+) Length   497 a.a.
NCBI ID   WP_000249322.1    Uniprot ID   A7ZRJ4
Organism   Escherichia coli strain UPEC-245U/190328     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1822715..1834208
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  G9W35_RS08755 (G9W35_08695) pilD 1823363..1824172 (+) 810 WP_001297673.1 prepilin peptidase PppA Machinery gene
  G9W35_RS08760 (G9W35_08700) gspS2 1824238..1824648 (+) 411 WP_021564872.1 type II secretion system pilot lipoprotein GspS-beta -
  G9W35_RS08765 (G9W35_08705) gspC 1824666..1825625 (+) 960 WP_000135067.1 type II secretion system protein GspC -
  G9W35_RS08770 (G9W35_08710) gspD 1825655..1827715 (+) 2061 WP_000498824.1 type II secretion system secretin GspD -
  G9W35_RS08775 (G9W35_08715) pilB/pilB1 1827715..1829208 (+) 1494 WP_000249322.1 type II secretion system ATPase GspE Machinery gene
  G9W35_RS08780 (G9W35_08720) gspF 1829208..1830431 (+) 1224 WP_001173453.1 type II secretion system inner membrane protein GspF -
  G9W35_RS08785 (G9W35_08725) gspG 1830448..1830903 (+) 456 WP_001087296.1 type II secretion system major pseudopilin GspG -
  G9W35_RS08790 (G9W35_08730) gspH 1830907..1831470 (+) 564 WP_001115154.1 type II secretion system minor pseudopilin GspH -
  G9W35_RS08795 (G9W35_08735) gspI 1831467..1831838 (+) 372 WP_000820104.1 type II secretion system minor pseudopilin GspI -
  G9W35_RS08800 (G9W35_08740) gspJ 1831835..1832440 (+) 606 WP_021534527.1 type II secretion system minor pseudopilin GspJ -
  G9W35_RS08805 (G9W35_08745) gspK 1832437..1833414 (+) 978 WP_000633208.1 type II secretion system minor pseudopilin GspK -

Sequence

Protein


Download         Length: 497 a.a.        Molecular weight: 55216.41 Da        Isoelectric Point: 6.0390

>NTDB_id=375441 G9W35_RS08775 WP_000249322.1 1827715..1829208(+) (pilB/pilB1) [Escherichia coli strain UPEC-245U/190328]
MVPVAQETTANTVRLPYSFSRRFSLVAWCEASLEILHVHPLSLSVLQELQRGLNAPFTLRQIDEAEFEQRLNAVWQRDSS
EARQLMEDLGSAEDFFILAEELPETEDLLESDDDAPIIKLINAMLAEAIKEGASDIHIETFEKSLVIRFRVDGTLHEMLR
PGRKLASLLVSRIKVMARLDIAEKRVPQDGRIALLLGGRAIDVRVSTMPSAWGERVVLRLLDKNQARLTLERLGLSQQLT
AQLRQLLHKPHGIFLVTGPTGSGKSTTLYAGLQELNNHSRNILTVEDPIEYMIEGIGQTQVNTRVGMTFARGLRAILRQD
PDVVMVGEIRDTETAEIAVQASLTGHLVLSTLHTNTAVGAITRLQDMGVEPFLLSSSLTGVMAQRLVRTLCPDCRQPAPA
TDEEKRLLGITDARTVTLYHPQGCPACNHKGFRGRTAIHELIVVDATLRDLIHRQAGELELERYVRQHSAGIRSNGIEKV
LAGETSLDEVLRVTMEA

Nucleotide


Download         Length: 1494 bp        

>NTDB_id=375441 G9W35_RS08775 WP_000249322.1 1827715..1829208(+) (pilB/pilB1) [Escherichia coli strain UPEC-245U/190328]
ATGGTGCCTGTAGCACAGGAAACCACCGCCAACACCGTGCGTCTGCCCTACAGTTTCAGCCGTCGGTTTAGCCTGGTGGC
ATGGTGCGAAGCGTCGCTGGAGATCCTCCACGTTCATCCGCTATCGCTCTCTGTTTTGCAGGAGCTGCAGCGGGGGCTGA
ACGCGCCCTTTACGCTGCGGCAAATCGACGAGGCCGAATTTGAACAGCGGCTGAATGCGGTCTGGCAGCGGGACTCTTCC
GAGGCTCGCCAGCTGATGGAAGATCTCGGTTCTGCCGAGGACTTTTTTATCCTCGCGGAAGAACTGCCGGAAACGGAAGA
TCTGCTGGAAAGCGACGACGATGCGCCGATCATCAAACTGATCAACGCCATGCTGGCAGAGGCAATCAAAGAAGGCGCTT
CGGATATCCACATTGAGACGTTTGAAAAGAGTCTGGTGATCCGTTTTCGTGTTGACGGCACATTACATGAAATGCTGCGT
CCGGGGCGCAAACTGGCCTCGCTGCTGGTGTCGCGTATCAAGGTGATGGCGCGGCTGGACATTGCCGAAAAGCGCGTGCC
GCAGGATGGACGTATTGCGCTGTTGCTGGGCGGCCGGGCGATTGACGTGCGTGTCTCCACCATGCCTTCTGCCTGGGGCG
AGCGCGTGGTGCTGCGACTGCTGGACAAAAACCAGGCCCGCCTGACGCTGGAGCGTCTGGGGCTTAGCCAGCAACTGACC
GCGCAGTTGCGCCAGCTGTTACACAAACCGCACGGCATCTTTCTGGTGACGGGGCCGACGGGTTCCGGCAAAAGCACCAC
GCTGTACGCCGGATTGCAGGAGCTGAACAACCATTCGCGCAACATTCTCACGGTTGAAGATCCCATCGAATACATGATTG
AAGGGATCGGTCAGACGCAGGTTAACACCCGCGTCGGCATGACCTTTGCCCGTGGGCTGCGCGCGATTTTGCGTCAGGAC
CCGGATGTGGTGATGGTCGGTGAAATCCGCGATACCGAAACCGCAGAAATCGCCGTCCAGGCTTCACTTACCGGACACCT
GGTCCTTTCCACGCTGCATACCAACACAGCGGTGGGGGCGATCACACGTTTGCAGGATATGGGCGTGGAGCCTTTCCTGC
TCTCTTCCAGTCTGACGGGCGTGATGGCGCAGCGACTGGTTCGCACGCTGTGTCCCGACTGCCGCCAGCCCGCACCAGCC
ACTGACGAAGAAAAACGCCTGCTGGGAATTACCGACGCCCGTACCGTCACTCTGTACCATCCACAGGGCTGTCCCGCCTG
TAATCACAAAGGTTTTCGCGGACGGACTGCCATCCATGAGCTGATCGTGGTGGATGCCACATTGCGTGATTTGATCCACC
GTCAGGCCGGGGAGCTGGAGCTGGAACGTTATGTCCGACAACACTCTGCGGGTATCCGCAGCAACGGCATTGAGAAAGTG
CTCGCCGGAGAAACCTCTCTCGATGAAGTTCTGCGGGTAACCATGGAGGCGTAA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(123-389)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A7ZRJ4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

39.372

100

0.429
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

47.765

85.513

0.408
  pilB Vibrio parahaemolyticus RIMD 2210633

48.166

82.294

0.396
  pilB Legionella pneumophila strain ERS1305867

43.852

86.72

0.38
  pilB Vibrio campbellii strain DS40M4

47.475

79.678

0.378
  pilB Acinetobacter baumannii D1279779

46.617

80.282

0.374
  pilB Vibrio cholerae strain A1552

46.212

79.678

0.368
  pilF Thermus thermophilus HB27

45

80.483

0.362

Multiple sequence alignment