Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   FP435_RS06615 Genome accession   NZ_CP041987
Coordinates   1224968..1226347 (-) Length   459 a.a.
NCBI ID   WP_265483440.1    Uniprot ID   -
Organism   Lactobacillus sp. PV037     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1219968..1231347
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FP435_RS06590 (FP435_06530) - 1219995..1220558 (-) 564 WP_265483430.1 sigma-70 family RNA polymerase sigma factor -
  FP435_RS06595 (FP435_06535) rlmB 1220710..1221465 (-) 756 WP_265483432.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  FP435_RS06600 (FP435_06540) - 1221458..1221889 (-) 432 WP_265483434.1 Mini-ribonuclease 3 -
  FP435_RS06605 (FP435_06545) cysS 1221882..1223297 (-) 1416 WP_265483436.1 cysteine--tRNA ligase -
  FP435_RS06610 (FP435_06550) gltX 1223387..1224886 (-) 1500 WP_265483438.1 glutamate--tRNA ligase -
  FP435_RS06615 (FP435_06555) radA 1224968..1226347 (-) 1380 WP_265483440.1 DNA repair protein RadA Machinery gene
  FP435_RS06620 (FP435_06560) - 1226347..1226898 (-) 552 WP_265483441.1 dUTP diphosphatase -
  FP435_RS06625 (FP435_06565) pepC 1227042..1228391 (+) 1350 WP_265483443.1 aminopeptidase C -
  FP435_RS06630 (FP435_06570) - 1228430..1229365 (-) 936 WP_265483445.1 AAA family ATPase -
  FP435_RS06635 (FP435_06575) - 1229459..1230136 (-) 678 WP_265483446.1 2,3-bisphosphoglycerate-dependent phosphoglycerate mutase -
  FP435_RS06640 (FP435_06580) - 1230149..1230376 (-) 228 WP_416209741.1 hypothetical protein -
  FP435_RS06645 (FP435_06585) - 1230376..1230654 (-) 279 WP_265483447.1 hypothetical protein -

Sequence

Protein


Download         Length: 459 a.a.        Molecular weight: 50168.92 Da        Isoelectric Point: 7.7214

>NTDB_id=375196 FP435_RS06615 WP_265483440.1 1224968..1226347(-) (radA) [Lactobacillus sp. PV037]
MAKVKTQYKCRSCGYISASYLGRCPNCGAWNQFEKESIQSSKTSTKATPSRLMTKVGLNEPVKLNEVKAEKETRIKTEFE
ELNRVLGGGIVPGSLVLIGGDPGIGKSTLMLQIMGAIAKKHKVLYVSGEESASQIKMRADRLNVAESGMLLYPETNMENI
RHQIDEIKPKFLVIDSIQTMNEPSLDSVVGSAAQVKEVTNELMKIAKNDEITVFVVGHVTKEGAIAGPKILEHMVDTVLY
FEGDGHHAYRMLRSVKNRFGATNEMGMFEMENEGLKEVTNPSEIFLDERLPDSTGSAIVVSLEGTRPIMADIQALVAPTA
FGYAKRTTSGLDYNRVALLLAVLEKRGNLMLQNQDAFLTATGGIKLNEPAIDLAIAMAVASSYKNEGIPATDCFVGEIGL
TGEVRRVNQIQARVNEAAKIGFKRIFIPRHNLSTDLKNRTDIEVIGISSLAQALKLVFG

Nucleotide


Download         Length: 1380 bp        

>NTDB_id=375196 FP435_RS06615 WP_265483440.1 1224968..1226347(-) (radA) [Lactobacillus sp. PV037]
ATGGCAAAAGTTAAAACTCAATATAAATGTAGAAGTTGTGGATATATTTCTGCCTCATATTTGGGGCGTTGTCCTAATTG
TGGGGCCTGGAATCAGTTTGAAAAAGAAAGTATTCAAAGCTCTAAGACTTCTACTAAAGCAACTCCCAGTCGTTTAATGA
CCAAAGTAGGATTAAATGAGCCAGTAAAACTTAATGAAGTAAAGGCAGAAAAAGAAACACGAATTAAGACAGAATTTGAA
GAGTTAAATCGGGTTTTAGGTGGAGGAATTGTGCCGGGATCACTTGTACTAATTGGTGGAGATCCAGGAATTGGTAAGTC
GACTTTAATGTTACAAATCATGGGTGCAATTGCAAAGAAACACAAAGTTTTGTATGTTTCAGGAGAAGAGTCAGCAAGTC
AAATTAAGATGAGAGCTGATCGTTTAAATGTGGCTGAGTCGGGGATGCTTTTGTACCCAGAGACAAATATGGAAAATATT
CGGCATCAGATTGATGAAATAAAGCCGAAATTTTTAGTAATTGATTCTATTCAAACGATGAACGAACCGAGTCTTGATTC
GGTGGTAGGATCAGCTGCCCAGGTCAAAGAAGTTACCAATGAATTAATGAAGATTGCAAAAAATGATGAAATTACAGTTT
TTGTAGTTGGACATGTGACCAAGGAAGGGGCAATTGCTGGGCCAAAGATTTTAGAACATATGGTAGATACTGTTTTGTAT
TTTGAAGGAGATGGGCATCATGCTTATCGAATGTTACGGTCTGTAAAAAATCGTTTTGGAGCCACTAATGAAATGGGGAT
GTTTGAAATGGAAAATGAAGGATTAAAAGAAGTAACTAATCCGTCAGAAATTTTCCTAGATGAACGTTTACCTGATTCAA
CTGGTTCAGCAATTGTAGTGTCGTTAGAAGGAACAAGGCCCATTATGGCTGATATTCAGGCATTAGTAGCGCCCACTGCT
TTTGGTTATGCCAAAAGGACTACTTCTGGACTTGATTATAATCGTGTTGCCCTTTTGTTAGCTGTGCTTGAAAAAAGAGG
TAACTTAATGTTACAGAATCAAGATGCTTTTTTAACGGCAACAGGTGGAATTAAATTAAATGAACCAGCAATTGATTTAG
CTATTGCAATGGCAGTGGCTTCTAGTTATAAAAATGAAGGAATTCCTGCAACAGATTGCTTTGTGGGAGAAATCGGCTTG
ACGGGTGAAGTTAGAAGAGTCAATCAAATTCAAGCAAGAGTTAATGAAGCCGCAAAGATAGGCTTTAAACGAATTTTTAT
TCCCCGTCATAATTTATCAACTGACCTTAAAAATCGAACCGATATTGAAGTAATAGGAATTTCAAGTTTAGCCCAAGCTT
TAAAGCTAGTTTTTGGATAA

Domains

Predicted by InterproScan.

Subunit ChlI of Mg-chelatase (ChlI)

(364-431)

AAA domain (AAA_25)

(68-223)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-34)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus mitis SK321

58.079

99.782

0.58
  radA Streptococcus mitis NCTC 12261

58.079

99.782

0.58
  radA Streptococcus pneumoniae Rx1

58.079

99.782

0.58
  radA Streptococcus pneumoniae D39

58.079

99.782

0.58
  radA Streptococcus pneumoniae R6

58.079

99.782

0.58
  radA Streptococcus pneumoniae TIGR4

58.079

99.782

0.58
  radA Bacillus subtilis subsp. subtilis str. 168

55.435

100

0.556

Multiple sequence alignment