Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   FP432_RS07530 Genome accession   NZ_CP041982
Coordinates   1484333..1485712 (+) Length   459 a.a.
NCBI ID   WP_265488701.1    Uniprot ID   -
Organism   Lactobacillus sp. PV034     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1479333..1490712
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FP432_RS07500 (FP432_07395) - 1480023..1480214 (+) 192 WP_265488693.1 hypothetical protein -
  FP432_RS07505 (FP432_07400) - 1480230..1480439 (+) 210 WP_265488695.1 hypothetical protein -
  FP432_RS07510 (FP432_07405) - 1480449..1481126 (+) 678 WP_265488696.1 2,3-bisphosphoglycerate-dependent phosphoglycerate mutase -
  FP432_RS07515 (FP432_07410) - 1481252..1482187 (+) 936 WP_265488698.1 AAA family ATPase -
  FP432_RS07520 (FP432_07415) pepC 1482238..1483587 (-) 1350 WP_265488699.1 aminopeptidase C -
  FP432_RS07525 (FP432_07420) - 1483776..1484318 (+) 543 WP_265488700.1 dUTP diphosphatase -
  FP432_RS07530 (FP432_07425) radA 1484333..1485712 (+) 1380 WP_265488701.1 DNA repair protein RadA Machinery gene
  FP432_RS07535 (FP432_07430) gltX 1485785..1487284 (+) 1500 WP_265488702.1 glutamate--tRNA ligase -
  FP432_RS07540 (FP432_07435) cysS 1487377..1488795 (+) 1419 WP_265488703.1 cysteine--tRNA ligase -
  FP432_RS07545 (FP432_07440) - 1488788..1489231 (+) 444 WP_265488704.1 Mini-ribonuclease 3 -
  FP432_RS07550 (FP432_07445) rlmB 1489218..1489970 (+) 753 WP_265488705.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  FP432_RS07555 (FP432_07450) - 1490122..1490676 (+) 555 WP_265488706.1 sigma-70 family RNA polymerase sigma factor -

Sequence

Protein


Download         Length: 459 a.a.        Molecular weight: 50015.56 Da        Isoelectric Point: 8.3097

>NTDB_id=375166 FP432_RS07530 WP_265488701.1 1484333..1485712(+) (radA) [Lactobacillus sp. PV034]
MAKVKTRYKCRNCGYISASYLGRCPNCGAWNQFEKETEQVKTVSTKATASRLMTSTGSNEPVNLKNIKTHKEERLVTPFE
ELNRVLGGGIVPGSLVLIGGDPGIGKSTLMLQISSALAKDNKVLYVSGEESANQIKLRADRLHVGESGILLYPETNMENI
RQQIDDLKPDFLVIDSIQTMNEPSLDSMVGSASQVREVTSELMKIAKNDQITVFVIGHVTKEGSIAGPKILEHMVDTVLY
FEGDSNHSYRMLRSVKNRFGAANEIGMFEMKTEGLSEVTNPSAIFLDERLPNSTGSAVVVSLEGTRPILADIQALVTPTA
FGYAKRTTSGLDYNRVALLLAVLEKRGNLMLQNQDAFLTATGGIKLNEPAVDLAIAMAVASSYKNKEIAATDCFVGELGL
TGEVRKVNQIEARVNEAAKVGFKRIFVPKHNMTNKLKANDKIEVIGVASLPQTLKLVFG

Nucleotide


Download         Length: 1380 bp        

>NTDB_id=375166 FP432_RS07530 WP_265488701.1 1484333..1485712(+) (radA) [Lactobacillus sp. PV034]
ATGGCAAAGGTTAAAACACGCTATAAATGCCGTAATTGTGGCTATATTTCAGCGTCTTATTTAGGACGTTGTCCTAATTG
TGGTGCTTGGAATCAATTCGAAAAGGAAACTGAACAAGTAAAGACAGTTTCAACAAAAGCAACTGCTTCACGTTTAATGA
CAAGTACCGGTAGTAATGAACCAGTTAATTTAAAAAATATTAAGACCCATAAAGAAGAAAGATTGGTTACACCATTTGAA
GAACTAAATCGTGTGTTGGGTGGCGGAATAGTTCCTGGATCGTTAGTCCTAATTGGAGGAGACCCAGGTATCGGAAAATC
CACTTTAATGTTGCAGATTTCTAGTGCGCTGGCAAAGGATAATAAAGTTCTTTATGTTTCTGGGGAAGAATCGGCTAATC
AAATTAAGCTACGGGCTGATCGCTTGCATGTTGGAGAGAGTGGTATTTTACTTTATCCAGAAACAAATATGGAAAATATC
CGCCAACAAATTGACGATCTTAAACCTGATTTTTTAGTAATTGATTCAATCCAAACCATGAATGAACCATCATTAGATTC
TATGGTGGGATCAGCTTCTCAAGTACGTGAAGTAACTAGTGAACTTATGAAAATTGCCAAAAATGATCAAATTACTGTTT
TTGTGATTGGACATGTCACTAAAGAAGGTTCAATTGCAGGACCAAAGATTCTGGAACATATGGTTGACACTGTGCTTTAC
TTTGAGGGTGATAGTAATCATTCTTACCGGATGTTACGTTCCGTAAAGAATCGCTTTGGAGCGGCAAATGAAATTGGAAT
GTTTGAAATGAAAACTGAGGGCTTATCAGAAGTAACTAATCCTTCGGCGATCTTTTTAGATGAACGTTTACCTAATTCTA
CCGGATCAGCAGTCGTTGTTTCTTTAGAAGGAACACGACCAATTTTAGCGGATATTCAAGCTTTAGTAACTCCAACAGCA
TTTGGTTATGCTAAAAGAACTACTTCAGGGTTAGATTATAACCGAGTTGCACTTTTATTGGCTGTCTTAGAAAAGCGGGG
CAATTTAATGTTGCAGAATCAAGATGCTTTTTTGACTGCAACTGGTGGAATTAAATTAAATGAGCCAGCTGTTGATTTGG
CAATTGCAATGGCAGTTGCTTCAAGTTACAAGAATAAAGAAATTGCGGCTACTGATTGTTTTGTAGGAGAATTAGGATTA
ACTGGGGAAGTAAGAAAGGTTAATCAAATTGAAGCAAGAGTAAATGAAGCTGCAAAAGTTGGTTTTAAGAGAATTTTTGT
TCCAAAACATAATATGACTAATAAATTAAAAGCTAATGACAAGATTGAAGTAATTGGAGTAGCAAGTTTACCCCAAACTT
TAAAGTTAGTTTTCGGATAG

Domains

Predicted by InterproScan.

Subunit ChlI of Mg-chelatase (ChlI)

(364-431)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-34)

AAA domain (AAA_25)

(68-223)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus mitis NCTC 12261

58.079

99.782

0.58
  radA Streptococcus pneumoniae Rx1

58.079

99.782

0.58
  radA Streptococcus pneumoniae D39

58.079

99.782

0.58
  radA Streptococcus pneumoniae R6

58.079

99.782

0.58
  radA Streptococcus pneumoniae TIGR4

58.079

99.782

0.58
  radA Streptococcus mitis SK321

58.079

99.782

0.58
  radA Bacillus subtilis subsp. subtilis str. 168

54.565

100

0.547

Multiple sequence alignment