Detailed information    

insolico Bioinformatically predicted

Overview

Name   recJ   Type   Machinery gene
Locus tag   G8B43_RS05770 Genome accession   NZ_CP049689
Coordinates   1125511..1127721 (-) Length   736 a.a.
NCBI ID   WP_011528535.1    Uniprot ID   -
Organism   Streptococcus pyogenes strain ABC221     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1120511..1132721
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  G8B43_RS05740 (G8B43_05770) - 1120743..1121849 (-) 1107 WP_011528537.1 FAD-binding oxidoreductase -
  G8B43_RS05745 (G8B43_05775) - 1121889..1122677 (-) 789 WP_021299315.1 Nif3-like dinuclear metal center hexameric protein -
  G8B43_RS05750 (G8B43_05780) - 1122667..1123353 (-) 687 WP_002990104.1 tRNA (adenine(22)-N(1))-methyltransferase TrmK -
  G8B43_RS05755 (G8B43_05785) nth 1123425..1124081 (-) 657 WP_002990106.1 endonuclease III -
  G8B43_RS05760 (G8B43_05790) - 1124078..1124761 (-) 684 WP_011184446.1 DnaD domain-containing protein -
  G8B43_RS05765 (G8B43_05795) - 1124842..1125360 (-) 519 WP_002990109.1 adenine phosphoribosyltransferase -
  G8B43_RS05770 (G8B43_05800) recJ 1125511..1127721 (-) 2211 WP_011528535.1 single-stranded-DNA-specific exonuclease RecJ Machinery gene
  G8B43_RS05775 (G8B43_05805) - 1127718..1128482 (-) 765 WP_002984893.1 SDR family oxidoreductase -
  G8B43_RS05780 (G8B43_05810) rnz 1128482..1129411 (-) 930 WP_009881223.1 ribonuclease Z -
  G8B43_RS05785 (G8B43_05815) - 1129426..1130061 (-) 636 WP_002990114.1 cystathionine beta-lyase -
  G8B43_RS05790 (G8B43_05820) hflX 1130054..1131292 (-) 1239 WP_010922191.1 GTPase HflX -
  G8B43_RS05795 (G8B43_05825) miaA 1131365..1132264 (-) 900 WP_011528534.1 tRNA (adenosine(37)-N6)-dimethylallyltransferase MiaA -
  G8B43_RS05800 (G8B43_05830) - 1132398..1132574 (+) 177 WP_021299313.1 DUF3042 family protein -

Sequence

Protein


Download         Length: 736 a.a.        Molecular weight: 82633.54 Da        Isoelectric Point: 4.7553

>NTDB_id=374412 G8B43_RS05770 WP_011528535.1 1125511..1127721(-) (recJ) [Streptococcus pyogenes strain ABC221]
MIKSKYSWKIKDKKPDDGFFKLAKTKGLTQTAAQLIYERGIRTEEALDEFLTADLSQLHDPYLLHDMAKAVPRIRQAIEE
GERILIYGDYDADGMTSASIVKETLDMMGAEPLVYLPNRFTDGYGPNQSVYKYFIEQEAVSLIITVDNGVAGHEAIRYAQ
EQEVDVIVTDHHSLPEELPEAFAIIHPEHPDADYPFKHLAGCGVAFKLATALLESLPTDCLDLVAIGTIADMVSLTGENR
VLVKNGLAMLKHTERVGLQELMSLSPIDLEHFNEDAIGFQIAPQLNALGRLDDPNPAIELLTGFDDQEAQAIALMIKKKN
EERKALVQDIFDQAMAMVDPQKPVQVLAQAGWHPGVLGIVAGRIMETIGQTVVVLTIDNGFAKGSARSLEAINIFEALNG
KRELFTAFGGHAGAAGMTLPVDNLEALSDFLCQFVIERGLDQTAKNTLTIDERLSLDDLSLDILKSLDKLAPYGMDHQKP
VFYVKDIRVSQVRTIGQDQSHLKFKVSQGKASFDVLAFGQGSQLQEFRQATGLELAVTLSVNHWNGNTSLQFMLVDARVD
GVQLLDLRTKTAKVPEGIPTIEEDPNARVILINDIPEDFKTWRNQFVHKDFDAIYFKNQMKYPYYLTGFGSREQFAKLYK
TIYQFPEFDLRHKLTELSHYLNIEKLLLIKLIQIFEELSFVTIDDGLMTVNPQAQKREISESHIYQDLKELVKFQEIMAL
ASPKEMYDYLVKSNDQ

Nucleotide


Download         Length: 2211 bp        

>NTDB_id=374412 G8B43_RS05770 WP_011528535.1 1125511..1127721(-) (recJ) [Streptococcus pyogenes strain ABC221]
ATGATTAAATCCAAATATAGCTGGAAAATAAAAGACAAGAAGCCAGATGATGGCTTCTTTAAGCTAGCAAAGACAAAAGG
TCTAACCCAGACCGCCGCTCAGTTGATTTATGAGCGAGGGATAAGGACTGAAGAAGCCTTAGATGAGTTTTTAACAGCGG
ACTTGTCTCAACTGCATGATCCTTATTTGCTACATGACATGGCAAAAGCTGTGCCAAGAATTCGTCAAGCTATTGAAGAG
GGCGAACGAATTCTCATTTACGGCGACTATGATGCTGATGGCATGACGTCGGCTTCTATTGTCAAAGAAACCCTAGACAT
GATGGGAGCAGAGCCTTTGGTTTATCTTCCCAACCGCTTTACAGATGGCTATGGCCCCAACCAGAGCGTCTATAAATATT
TTATTGAGCAGGAAGCTGTGTCCCTAATTATCACGGTTGATAATGGCGTGGCCGGACATGAAGCGATTAGATATGCTCAA
GAACAGGAAGTTGATGTCATTGTAACTGACCATCATAGCCTGCCTGAAGAATTGCCAGAAGCCTTTGCCATCATTCATCC
TGAGCATCCTGATGCTGATTATCCCTTTAAACACTTAGCTGGCTGTGGCGTTGCCTTTAAATTGGCAACAGCTTTGCTAG
AAAGTTTACCAACAGACTGTCTAGACTTGGTAGCTATTGGAACCATTGCCGATATGGTAAGCTTGACAGGTGAAAACCGT
GTTTTAGTCAAAAATGGTCTGGCCATGCTCAAACACACCGAAAGAGTGGGCTTGCAGGAGTTGATGTCCTTGTCTCCTAT
TGATTTGGAACATTTTAATGAAGACGCCATTGGCTTTCAGATTGCTCCGCAATTAAATGCTTTAGGGCGCTTAGATGACC
CTAATCCAGCCATTGAGCTACTGACGGGCTTTGATGACCAAGAAGCTCAAGCGATTGCCTTGATGATTAAGAAAAAAAAT
GAAGAGCGTAAGGCCTTAGTCCAAGACATTTTTGATCAAGCGATGGCTATGGTTGATCCTCAAAAACCTGTTCAAGTCTT
GGCACAAGCTGGCTGGCATCCTGGTGTTCTTGGGATTGTCGCTGGTCGTATCATGGAAACTATTGGACAAACGGTCGTCG
TTTTGACCATTGATAATGGCTTTGCAAAAGGCTCGGCCAGAAGTTTAGAAGCTATTAATATTTTTGAAGCCTTGAATGGC
AAGCGTGAGTTATTTACAGCCTTTGGGGGACATGCTGGTGCAGCAGGAATGACCCTGCCCGTTGACAACTTGGAAGCTCT
CTCAGACTTTCTTTGTCAGTTTGTGATAGAACGTGGCTTAGATCAGACTGCCAAAAATACGTTAACTATTGATGAAAGAC
TGTCTTTGGACGACCTTAGCCTTGATATTTTAAAGAGTTTAGATAAATTAGCCCCTTATGGGATGGATCATCAAAAGCCG
GTCTTTTATGTCAAGGACATAAGGGTTAGTCAAGTGCGTACCATAGGGCAAGACCAGAGCCATCTTAAATTTAAAGTCAG
TCAGGGCAAAGCTTCCTTTGACGTACTGGCTTTTGGTCAAGGAAGTCAACTGCAAGAGTTCCGACAAGCTACTGGTTTAG
AATTAGCAGTTACCTTATCTGTCAACCATTGGAACGGGAACACGAGTCTCCAATTCATGTTAGTAGATGCTCGTGTTGAC
GGAGTGCAACTTCTTGACTTAAGGACAAAAACAGCCAAAGTTCCTGAAGGTATTCCGACTATTGAAGAAGATCCAAATGC
CAGAGTCATTCTGATTAATGACATCCCTGAAGATTTCAAAACCTGGCGCAACCAGTTTGTTCACAAGGATTTTGACGCCA
TCTATTTTAAAAATCAGATGAAATATCCTTATTATTTAACAGGATTTGGCAGCAGAGAGCAGTTTGCCAAGCTCTACAAA
ACCATCTATCAGTTTCCTGAATTTGATTTACGGCATAAATTGACAGAGTTGAGTCACTATTTAAACATAGAAAAACTTTT
ACTGATTAAGTTGATTCAAATTTTTGAAGAGTTGTCTTTTGTGACGATTGACGATGGGCTGATGACGGTTAACCCCCAAG
CGCAAAAACGAGAGATTTCAGAGAGCCACATTTACCAAGACTTAAAAGAATTGGTGAAATTTCAAGAAATCATGGCTTTG
GCTAGCCCGAAAGAGATGTATGATTATTTGGTGAAATCAAACGATCAATAA

Domains

Predicted by InterProScan.

Single-strand DNA-specific exonuclease, C terminal domain (ssDNA-exonuc_C)

(588-729)

RecJ OB domain (RecJ_OB)

(450-556)

DHHA1 domain (DHHA1)

(317-435)

DHH family (DHH)

(83-228)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recJ Streptococcus pneumoniae strain D39V

63.008

100

0.632
  recJ Bacillus subtilis subsp. subtilis str. 168

34.994

100

0.372